<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> Morphological lesions in hepatic stellate cells caused by the immunosuppressive doses of dexamethasone were investigated in dogs. The archival samples of liver collected during a surgical biopsy were examined. The samples were fixed in 10% buffered formalin or Carnoy’s solution and then stained with routine histochemical methods. The lesions were also investigated under electron microscope. It was demonstrated that the number of stellate cells significantly increased (P = 0.0277), yet the size of cytoplasmic lipid droplets significantly decreased (P = 0.0001). Even though steroid-induced hepatopathy is considered to be a reversible pathology, and the lesions in hepatocytes under the influence of dexamethasone occur in a short period, it was found that hepatic stellate cells proliferated and underwent activation. This resulted in collagen accumulation in the hepatic sinuses. The functional and morphological disturbances in the canine liver in the course of steroid-induced hepatopathy are initially subclinical, but the changes in the structure and function of hepatic stellate cells may become a cause of lesions in the wall of hepatic sinusoidal vessels, which may induce additional functional pathologies unrelated to the damage to hepatocytes.</p>
</abstract>

Morphological lesions in hepatic stellate cells caused by the immunosuppressive doses of dexamethasone were investigated in dogs. The archival samples of liver collected during a surgical biopsy were examined. The samples were fixed in 10% buffered formalin or Carnoy’s solution and then stained with routine histochemical methods. The lesions were also investigated under electron microscope. It was demonstrated that the number of stellate cells significantly increased (P = 0.0277), yet the size of cytoplasmic lipid droplets significantly decreased (P = 0.0001). Even though steroid-induced hepatopathy is considered to be a reversible pathology, and the lesions in hepatocytes under the influence of dexamethasone occur in a short period, it was found that hepatic stellate cells proliferated and underwent activation. This resulted in collagen accumulation in the hepatic sinuses. The functional and morphological disturbances in the canine liver in the course of steroid-induced hepatopathy are initially subclinical, but the changes in the structure and function of hepatic stellate cells may become a cause of lesions in the wall of hepatic sinusoidal vessels, which may induce additional functional pathologies unrelated to the damage to hepatocytes.

Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy

Department of Pathology and Veterinary Diagnostics

Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Laboratory of Veterinary Epidemiology and Economics

Division of Small Animal Internal Medicine, Department of Small Animal Diseases with Clinic; Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy"}

Morphological lesions in hepatic stellate cells caused by the immunosuppressive doses of dexamethasone were investigated in dogs. The archival samples of liver...

Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy

Published Online: Jun 17, 2014

Page range: 273 - 276

Received: Jul 20, 2013

Accepted: Jun 05, 2014

DOI: https://doi.org/10.2478/bvip-2014-0041

Keywords
dog, hepatic stellate cells, hepatopathy, dexamethasone.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy

Published Online: Jun 17, 2014

Page range: 273 - 276

Received: Jul 20, 2013

Accepted: Jun 05, 2014

DOI: https://doi.org/10.2478/bvip-2014-0041

Keywordsdog, hepatic stellate cells, hepatopathy, dexamethasone.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, hepatic stellate cells, hepatopathy, dexamethasone.