<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley’s medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.</p>
</abstract>

In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley’s medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Department of Agriculture Resources Utilization, College of Resources and Environments

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A...

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Published Online: Mar 25, 2014

Page range: 11 - 15

DOI: https://doi.org/10.2478/bvip-2014-0002

Keywords
goat, Mycoplasma mycoides cluster, isolation, biochemical characterisation, antimicrobial resistance.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Published Online: Mar 25, 2014

Page range: 11 - 15

DOI: https://doi.org/10.2478/bvip-2014-0002

Keywordsgoat, Mycoplasma mycoides cluster, isolation, biochemical characterisation, antimicrobial resistance.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
goat, Mycoplasma mycoides cluster, isolation, biochemical characterisation, antimicrobial resistance.