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Association of genetic polymorphisms in the Matrix Gla Protein (MGP) gene with coronary artery disease and serum mgp levels

S Karsli-Ceppioglu
S Karsli-Ceppioglu
, 
S Yazar
S Yazar
, 
Y Keskin
Y Keskin
, 
M Karaca
M Karaca
, 
NE Luleci
NE Luleci
 and 
T Yurdun
T Yurdun
   | Dec 21, 2019
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Article Category: Original article
Published Online: Dec 21, 2019
Page range: 43 - 50
DOI: https://doi.org/10.2478/bjmg-2019-0020
Keywords
© 2019 Karsli-Ceppioglu S, Yazar S, Keskin Y, Karaca M, Luleci NE, Yurdun T, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The structure of the human MGP gene showing the location of the rs4236, rs12304 and rs1800802 polymorphisms.
The structure of the human MGP gene showing the location of the rs4236, rs12304 and rs1800802 polymorphisms.

Figure 2

Histograms of the serum MGP concentrations (μg/L) relative to MGP genotypes. The statistical significance between MGP concentrations were indicated by letters ‘a’ and ‘b.’
Histograms of the serum MGP concentrations (μg/L) relative to MGP genotypes. The statistical significance between MGP concentrations were indicated by letters ‘a’ and ‘b.’

Figure 3

Linkage disequilibrium block structures of MGP gene polymorphisms was performed by Haploview (version 4.2) [12]. Numbers in squares (D’ values) indicate the pairwise correlation between polymorphisms. Color scheme from red to white represent higher to lower LD values.
Linkage disequilibrium block structures of MGP gene polymorphisms was performed by Haploview (version 4.2) [12]. Numbers in squares (D’ values) indicate the pairwise correlation between polymorphisms. Color scheme from red to white represent higher to lower LD values.

The baseline distribution characteristics of MGP alleles and their correlation with serum MGP concentrations. (Values are presented as mean ± SD.)

Parameters rs1800802 rs4236 rs12304 sMGP (μg/L)
TT+TT vs. CC p Valuea Thr/Thr/Ala+Thr Ala/vs. Ala p Valuea Glu/Glu/Glu X+X/vs. X p Valuea Correlation
Gender (F; M) F:22; M: 30; F: 42; M: 74 0.452 F: 9; M: 43; F: 40; M: 76 0.024 F: 39; M: 12; F: 94; M:22 0.500 0.043
LDL-cholesterol (mg/dL) 106.81±38.62; 113.93±41.19 0.701 117.61±42.17; 108.47±39.26 0.394 112.15±37.40; 112.21±53.30 0.878 0.222
HDL-cholesterol (mg/dL) 44.69±16.78; 43.13±11.31 0.556 46.87±14.79; 41.72±11.76 0.049 44.29±8.53; 41.25±8.53 0.361 0.145
Total cholesterol (mg/dL) 188.28±46.01; 185.56±51.14 0.344 195.10±45.91; 181.37±51.04 0.245 187.45±48.46; 185.10±53.94 0.939 0.085
Triglycerides (mg/dL) 170.81±83.15; 166.67±102.21 0.769 177.05±119.29; 162.67±81.21 0.528 162.09±84.13; 199.42±139.30 0.115 0.903
Fasting glucose (mg/dL) 121.31±42.67; 117.13±42.46 0.544 125.54±51.81; 114.28±35.63 0.539 117.80±39.23; 121.63±56.52 0.653 0.588
eGFR (mL/min./1.73m3) 85.5786.75±±23.6220.99 ; 0.835 88.8085.81±±22.0121.97 ; 0.405 85.9888.70±±21.3724.28 ; 0.377 0.015

Odds ratios and their 95% confidence intervals for alleles of matrix Gla protein genotypes for coronary artery disease.

Genotypes CAD Patients (n = 112) Single-Vessel Disease (n = 62) Two-Vessel Disease (n = 30) Three-Vessel Disease (n = 20)
OR (95% CI) p Valuea OR (95% CI) p Valuea OR (95% CI) p Valuea OR (95% CI) p Valuea
rs1800802: TT vs.
TT+CC 0.927 (0.480-1.792) 0.822 0.736 (0.346-1.567) 0.426 1.182 (0.481-2.905) 0.716 1.264 (0.451-3.457) 0.655
rs4236: Thr/Thr vs.
Thr/Ala+Ala/Ala 1.809 (0.854-3.829) 0.121 1.878 (0.821-4.294) 0.135 1.833 (0.680-4.943) 0.231 1.571 (0.498-4.962) 0.441
rs12304: Glu/Glu vs.
Glu/X+X/X 0.967 (0.433-2.162) 0.936 0.857 (0.352-2.086) 0.734 1.625 (0.469-5.631) 0.444 0.750 (0.224-2.512) 0.641

Genotype and allele distributions of matrix Gla protein gene rs1800802, rs4236, and rs12304 polymorphisms.

Genotype/Allele CAD Patients (n = 112) Controls (n =56) p Value
rs1800802
Genotype: TT 42 (37.5%) 22 (39.3%)
TC 55 (49.1%) 29 (51.8%) 0.701
CC 15 (13.4%) 5 (8.9%)
Allele: T 139 (62.0%) 73 (65.2%)
C 85 (38.0%) 39 (34.8%) 0.822
rs4236
Genotype: Thr/Thr 37 (33.0%) 12 (21.4%) 0.267
Thr/Ala 57 (50.9%) 35 (62.5%)
Ala/Ala 18 (16.1%) 9 (16.1%)
Allele: Thr 131 (58.5%) 59 (52.7%)
Ala 93 (41.5%) 53 (47.3%) 0.119
rs12304
Genotype: Glu/Glu 89 (79.5%) 44 (80.0%) 0.936
Glu/X 23 (20.5%) 11 (20.0%)
X/X 0 (0.0%) 0 (0.0%)
Allele: Glu 201 (89.7%) 99 (90.0%) 0.936
X 23 (10.3%) 11 (10.0%)

The main characteristics of the coronary artery disease patients and controls. (Values are presented as mean ± SD and percent.)

Parameters CAD Patients (n = 112) Controls (n = 56) p Valuea
Gender (F; M) F: 22; M: 90 F: 30; M: 26 <0.001
Age (years) 62.63 ± 9.16 56.40 ± 9.40 <0.001
Hypertension 76 (67.9%) 34 (60.7%) 0.384
DMT2 52 (46.2%) 20 (35.7%) 0.244
Current smoker 66 (58.9%) 29 (51.8%) 0.406
LDL-cholesterol (mg/dL) 114.99 ± 43.28 104.67 ± 32.47 0.463
HDL-cholesterol (mg/dL) 44.61 ± 13.49 41.33 ± 12.14 0.228
Total cholesterol (mg/dL) 190.81 ± 52.96 176.42 ± 39.45 0.310
Triglycerides (mg/dL) 176.22 ± 106.12 149.26 ± 68.27 0.234
Fasting glucose (mg/dL) 122.21 ± 47.81 109.79 ± 24.89 0.215
eGFR (mL/min./1.73m3) 90.37 ± 19.83 84.83 ± 22.81 0.051
sMGP (μg/L) 0.82 ± 0.37 0.87 ± 0.42 0.426
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