1. bookVolume 25 (2022): Issue 1 (June 2022)
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eISSN
2344-4592
First Published
20 Dec 2020
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2 times per year
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English
access type Open Access

The pig gut microbiota analysis techniques, a comparison

Published Online: 04 Jul 2022
Volume & Issue: Volume 25 (2022) - Issue 1 (June 2022)
Page range: 90 - 115
Journal Details
License
Format
Journal
eISSN
2344-4592
First Published
20 Dec 2020
Publication timeframe
2 times per year
Languages
English
Abstract

The gastrointestinal tract microbiota composition can be radically different among pigs with regards to their health, age, feed intake, breed, and local conditions making its investigation into an important and multifaceted tool for evaluating the effects of new breeding and feeding schemes and animal welfare. For a long time, scientists have relied upon classical microbiology techniques to identify the makeup of bacterial populations from the gastrointestinal tract. Since their conceptions, high throughput sequencing and other molecular techniques contributed immensely in better understanding the pig gut microbiota and its makeup. Since many techniques to identify and quantify the microbiota are based on bacterial gene targets, they can, be used to determine the relation between different bacterial taxons and developmental processes, how it influences the host metabolism or diseases impact. In our review, we offer an overview of the different methods employed in the assay of the gut microbiota of pigs along with differences and pitfalls.

Keywords

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