1. bookVolume 25 (2022): Issue 1 (June 2022)
Journal Details
License
Format
Journal
eISSN
2344-4592
First Published
20 Dec 2020
Publication timeframe
2 times per year
Languages
English
access type Open Access

Genetic evaluation of Teleorman Black Head ewes using genes polymorphism for improving milk traits

Published Online: 04 Jul 2022
Volume & Issue: Volume 25 (2022) - Issue 1 (June 2022)
Page range: 60 - 74
Journal Details
License
Format
Journal
eISSN
2344-4592
First Published
20 Dec 2020
Publication timeframe
2 times per year
Languages
English
Abstract

The purpose of the present study was to asses specific gene markers associated with the sheep milk production in Teleorman Black Head ewes. For the proper selection of the best candidates for milk production, the genetic markers such as CSN3, BLG and PRL were selected. To detect CSN3, PRL and BLG polymorphisms, genomic DNA was isolated from blood samples collected from 50 ewes. ASA-PCR and PCR-RFLP methods were used for genotyping of animals; the genotypes frequencies and Hardy-Weinberg equilibrium were estimated using R software. In our samples, for CSN3 gene were identified two genotypes (CC and CT), the most frequent being CT genotype (94%). For PRL gene, were identified three genotypes (AA, AB and BB), AA and BB genotypes being the most frequent (36% and 40% respectively). Also, three genotypes were identified for BLG gene (AA, AB and BB), with an increased frequency of AB genotype (44%). These preliminary data will be correlated in the next experiments with phenotypic data, generating important tools for genetic selection of the Teleorman Black Head sheep population.

Keywords

Ahmad H Al-Amareen, Khaleel J., 2010. Single and combined effects of CSN1S1 and CSN2-casein genes on Awassi sheep milk quantity and quality. Vet. World 15, 2, 10.14202/vetworld.2022.435-441. Search in Google Scholar

Arora, R., Bhatia, S., Mishra, B. P., Sharma, R., Pandey, A. K., Prakash, B. and Jain, A. 2010. Genetic Polymorphism of the b-Lactoglobulin Gene in Native Sheep from India. Biochem. Genet. 48, 304–311. Search in Google Scholar

Barillet, F., Arranz, J. J. and Carta, A., 2005. Mapping quantitative trait loci for milk production and genetic polymorphism of milk proteins in dairy sheep. Genet. Sel. Evol. 37, 1, 09-123.10.1186/1297-9686-37-S1-S109 Search in Google Scholar

Clement, P., Agboola, S., Bencini, R., 2006. A study of polymorphism in milk proteins from local and imported dairy sheep in Australia by capillary electrophoresis, LWT 39, 1, 63-69.10.1016/j.lwt.2004.11.004 Search in Google Scholar

Ceriotti G, Chessa S, Bolla P, Budelli E, Bianchi L, Duranti E, Caroli A. 2004. Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism. J Dairy Sci 87:2606–261310.3168/jds.S0022-0302(04)73386-X Search in Google Scholar

Ceriotti, G., Chiatti, F., Prasad B., Martin M., Caroli, A., 2010. Genetic variability of the ovine αs1-casein. Italian J. Ani. Sci. 4,2s, DOI: 10.4081/ijas.2005.2s.64. Open DOISearch in Google Scholar

Dario, C.; Carnicella, D. and Bufano, G., 2005. Effect of b-lactoglobulin genotypes on ovine milk composition in Altamurana breed. Archivos de Zootecnia. 54,105–108. Search in Google Scholar

Elmaci, C., Oner, Y. and Balcioglu, M.S., 2006. Genetic Polymorphism of β-Lactoglobulin Gene in Native Turkish Sheep Breeds. Bioche. Genet., 44, 379-384.10.1007/s10528-006-9036-z16969686 Search in Google Scholar

Feligini, M., Vlaco, S., Curik, V. C, Parma, P., Greppi, G., and Enne, G., 2005. A single nucleotide polymorphism in the sheep kappa-casein coding region. J. Dairy Res. 72, 317–321.10.1017/S002202990500093216174363 Search in Google Scholar

Giambra, I.J., Erhardt, G., 2012. Molecular genetic characterization of ovine CSN1S2 variants C and D reveal further important variability within CSN1S2. Animal Genet. 43, 5, 642-5.10.1111/j.1365-2052.2011.02299.x22497628 Search in Google Scholar

Graffelman J, Moreno V. The mid p-value in exact tests for Hardy-Weinberg equilibrium. Stat Appl Genet Mol Biol. 2013 Aug;12(4):433-48. doi: 10.1515/sagmb-2012-0039. PMID: 23934608.23934608 Open DOISearch in Google Scholar

Gras, M. A., Rotar, C. M., Pelmus, R. S., Lazar, C., Ghita, E., Grosu, H. 2017. Prolactin Polymorphism Effect over Production Traits Types at Transylvanian Merino Sheep, Sci. Papers: Anim. Sci. and Biotech. 50, 1, https://web.archive.org/web/20180423081226id_/http://www.spasb.ro/index.php/spasb/article/viewFile/2298/pdf. Search in Google Scholar

Gutierrez-Gil, B., El-Zarei, M.F., Alvarez, L., 2009. Quantitative trait loci underlying milk production traits in sheep. Anim. Genet., 40, 423–434.10.1111/j.1365-2052.2009.01856.x19397522 Search in Google Scholar

Hristov, P., Mitkov, I., Sirakova, D., Mehandgiiski, I., Radoslavov, G. 2016. Measurement of Casein Micelle Size in Raw Dairy Cattle Milk by Dynamic Light Scattering. Ed. InTech. Milk Proteins - From Structure to Biological Properties and Health Aspects. Chapt. 2, DOI: 10.5772/62779. Open DOISearch in Google Scholar

Lazăr, C., Pelmuş, R., Marin, D., Ghiţă, E., 2010. Preliminary investigations on milk proteins polymorphism in Carpathian goats and in F1 Carpathian Saanen hybrids, Arch. Zootech. 13,4, 45-57. Search in Google Scholar

Moatsou, G.; Hatzinaki, A.; Samolada, M. and Anifantakis, E., 2005. Major whey proteins in ovine and caprine acid wheys from indigenous Greek breeds. International Dairy, 15, 123–131.10.1016/j.idairyj.2004.06.005 Search in Google Scholar

Othman, O., Ahmed S., 2006. Genetic polymorphism detection of two alfa caseins genes in three Egyptian sheep breeds, J. Biol. Sci. 6, 2, 238-241. Search in Google Scholar

Othman, O., El-Fiky, S., Hassan, N., Mahfouz, E., Balabel, E. A., 2013. Genetic variations of β- and K-casein genes in Egyptian sheep breeds. J. Genet. Engin. Biol. 11, 129-134.10.1016/j.jgeb.2013.05.001 Search in Google Scholar

Orford, M., Tzamaloukas, O., Papachristoforou, C., and Miltiadou, D., 2010. Technical note: a simplified PCR based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds. J. Dairy. Sci., 93, 5996–5999.10.3168/jds.2010-356921094774 Search in Google Scholar

Pelmus, R.S., Pistol, G.C., Lazar, C., Gras M.A., Ghita E. 2012. Preliminary study on milk composition and milk protein polymorphism in the Romanian local sheep breed Teleorman Black Head Tsigai. Roman. Biotech. Lett., 17, 5, http://www.rombio.eu/rbl5vol17/5%20Rodica%20Pelmus.pdf Search in Google Scholar

Pietrolà E, Carta A, Fraghì A, Piredda G, Pilla, F. 2000. Effect of β-lactoglobulin locus on milk yield in Sarda ewes. Zoot Nutr Anim 26:129–133 Search in Google Scholar

Pauciullo, A., Erhardt, G. and Uversky, V. N. 2015. Molecular Characterization of the Llamas (Lama glama) Casein Cluster Genes Transcripts (CSN1S1, CSN2, CSN1S2, CSN3) and Regulatory Regions. PLOS ONE, 10, 4, e0124963 DOI: 10.1371/journal.pone.0124963441441125923814 Open DOISearch in Google Scholar

Ramos, A.M., Matos, C.A.P., Russo-Almeida, P.A., Bettencourt, C.M.V., Matos, J., Martins, A., Pinheiro, C., and Rangel-Figueiredo, T., 2009. Candidate genes for milk production traits in Portuguese dairy sheep, Small Rum. Res., 82, 117–121.10.1016/j.smallrumres.2009.02.007 Search in Google Scholar

Rampilli M, Cecchi F, Giuliotti L, Cattaneo, TMP. 1997. The influence of β-lactoglobulin genetic polymorphism on protein distribution and coagulation properties in milk of Massese breed ewes. Milk Protein Polymorphism. International Dairy Federation, pp 311-315 Search in Google Scholar

Ramunno, L., Cosenza, G., Rando, A., Pauciullo, A., Illario, R., Gallo, D., Berardino, D. Di, Masina, P. 2005. Comparative analysis of gene sequence of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland. Gene. 345, 2, 289-99.doi: 10.1016/j.gene.2004.12.003.15716101 Open DOISearch in Google Scholar

Schlee P, Krause I, Rottmann, O. 1993. Genotyping of ovine beta-Lg alleles A and B using The PCR. Arch Tierz 36:519-523 Search in Google Scholar

Selvaggi M, Laudadio V, Dario C, Tufarelli V. 2014 Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production. J Sci Food Agric. 94(15):3090-9. doi: 10.1002/jsfa.6750.24862201 Open DOISearch in Google Scholar

Singh, L., Jayakumar, S., Anurodh Sharma, Shishir, Gupta K., Dixit S., Gupta N., Gupta, S. 2015. Comparative screening of single nucleotide polymorphisms in β-casein and κ-casein gene in different livestock breeds of India. Biol. Medicine, Meta Gene.10.1016/j.mgene.2015.03.005440181225905036 Search in Google Scholar

Staiger, E., Thonney, A., Buchanan, M.L., Rogers J.W., Oltenacu, E.R., Mateescu R.G., 2010. Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. J. Dairy Sci. 93, 4, 1736–1742.10.3168/jds.2009-263020338452 Search in Google Scholar

Lan, X.Y., Pan, Ch.Y., Chen, H., Lei, ChZ, Li, F.Y., Zhang, H.Y., Ni, Y.S. 2009. Novel SNP of the goat prolactin gene (PRL) associated with cashmere traits. J. Appl. Genet. 50, 1, 51-4, doi: 10.1007/BF03195652.19193983 Open DOISearch in Google Scholar

Wang, F. 2022. Regulation of milk synthesis in the bovine mammary gland: The role of MRCK-alfa in prolactin-and amino acid-evoked lactogenesis. Thesis. DOI: 10.18174/562090 Open DOISearch in Google Scholar

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