<abstract xmlns="http://www.w3.org/1999/xhtml">Co-contamination with mycotoxin represents a serious concern for human and animal health. In this <italic>in vitro</italic> study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed.</abstract>

Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed.

Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver

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Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA...

Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver

Published Online: Oct 02, 2020

Page range: 73 - 80

DOI: https://doi.org/10.2478/azibna-2020-0007

Keywords
zearalenone, ochratoxin, liver, co-contamination, HepG2

© 2020 D.E. Marin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver

Published Online: Oct 02, 2020

Page range: 73 - 80

DOI: https://doi.org/10.2478/azibna-2020-0007

Keywordszearalenone, ochratoxin, liver, co-contamination, HepG2

© 2020 D.E. Marin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
zearalenone, ochratoxin, liver, co-contamination, HepG2