<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to evaluate the phytochemical properties, antibacterial and anti free-radical activities of <italic>Retama raetam</italic> extracts which is growing in the South-East of the Algeria Desert. The chemical screening showed the presence of many secondary metabolites such as tannins catechin, sterols and terpenes, and the absence of gallic tannins compounds. The obtained results demonstrated that the methanolic extract has shown moderate total phenolic and flavonoids contents (31.59 ± 2.82 mg AG E/g extract and 14.35 ±1.02 mg Qu E/g extract respectively). In the free radical DPPH test, the values of IC<sub>50</sub> were converging in all extracts of <italic>R. raetam</italic>. The antibacterial activity of extracts has been tested against ten bacterial strains, were registered as the best inhibition zones with <italic>Vibrio cholera</italic>, <italic>Micrococcus luteus</italic> and <italic>Serratia marcescen</italic>. <italic>Pseudomonas aeruginosa</italic> ATCC 27853 and <italic>Staphylococcus aureus</italic> ATCC 25923 strains showed high resistance against most of all concentrations of extracts, and we noted the Gram-negative bacteria strains are the most sensitive to the different extracts of the plant. The qualitative analysis of extracts by using HPLC showed the contrast in presence of the phenolic compounds, such as in ethyl acetate extract registered absence of chlorogenic acid, also the absence of caffeic acid in 1-butanol extract. These results confirmed of the phenolic extracts of this plant are a source natural alternative to antibiotics and antioxidants.</p>
</abstract>

This study aimed to evaluate the phytochemical properties, antibacterial and anti free-radical activities of Retama raetam extracts which is growing in the South-East of the Algeria Desert. The chemical screening showed the presence of many secondary metabolites such as tannins catechin, sterols and terpenes, and the absence of gallic tannins compounds. The obtained results demonstrated that the methanolic extract has shown moderate total phenolic and flavonoids contents (31.59 ± 2.82 mg AG E/g extract and 14.35 ±1.02 mg Qu E/g extract respectively). In the free radical DPPH test, the values of IC50 were converging in all extracts of R. raetam. The antibacterial activity of extracts has been tested against ten bacterial strains, were registered as the best inhibition zones with Vibrio cholera, Micrococcus luteus and Serratia marcescen. Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923 strains showed high resistance against most of all concentrations of extracts, and we noted the Gram-negative bacteria strains are the most sensitive to the different extracts of the plant. The qualitative analysis of extracts by using HPLC showed the contrast in presence of the phenolic compounds, such as in ethyl acetate extract registered absence of chlorogenic acid, also the absence of caffeic acid in 1-butanol extract. These results confirmed of the phenolic extracts of this plant are a source natural alternative to antibiotics and antioxidants.

Phytochemical properties, antibacterial and anti-free radical activities of the phenolic extracts of <italic>Retama raetam</italic> (Forssk) Webb. &amp; Berthel. collected from Algeria Desert

Biology Department, Faculty of Natural Science and Life

Laboratory Biology, Environment and Health (LBEH)

Ovidius University Annals of Chemistry

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phytochemical properties, antibacterial and anti-free radical activities of the phenolic extracts of  (Forssk) Webb. & Berthel. collected from Algeria Desert

This study aimed to evaluate the phytochemical properties, antibacterial and anti free-radical activities of Retama raetam extracts which is growing in the...

Phytochemical properties, antibacterial and anti-free radical activities of the phenolic extracts of Retama raetam (Forssk) Webb. & Berthel. collected from Algeria Desert

Published Online: Apr 03, 2021

Page range: 33 - 39

Received: Nov 11, 2020

Accepted: Feb 22, 2021

DOI: https://doi.org/10.2478/auoc-2021-0005

Keywords
anti-free radical activity, antibacterial activity, HPLC

© 2021 Atef Chouikh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phytochemical properties, antibacterial and anti-free radical activities of the phenolic extracts of Retama raetam (Forssk) Webb. & Berthel. collected from Algeria Desert

Published Online: Apr 03, 2021

Page range: 33 - 39

Received: Nov 11, 2020

Accepted: Feb 22, 2021

DOI: https://doi.org/10.2478/auoc-2021-0005

Keywordsanti-free radical activity, antibacterial activity, HPLC

© 2021 Atef Chouikh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
anti-free radical activity, antibacterial activity, HPLC