<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Rheum</italic> species are important medicinal herbs, often used in pharmacological research, due to the presence of anthracene derivatives in the subterranean parts of the plant. In this study, we intended to assess its antioxidant capacity, in correlation with the method of extraction. For this purpose, <italic>Rheum ribes</italic> extraction was realized with four solvents of different polarities (50% methanol, 70% ethanol, 80% acetonitrile, and petroleum ether). We used different extraction techniques, such as orbital shaker, ultrasonic stirrer, microwave, and Soxhlet extraction, and the total phenolic content of the <italic>Rheum ribes</italic> extracts was determined by modified Folin–Ciocalteu method. The reducing power and radical scavenging activity of the extracts were also evaluated. The results shown that the antioxidant activity of the extracts depends on the extraction methods especially through the used solvent and decreases in the order: ethanol > methanol > acetonitrile > petroleum ether.</p></abstract>

Rheum species are important medicinal herbs, often used in pharmacological research, due to the presence of anthracene derivatives in the subterranean parts of the plant. In this study, we intended to assess its antioxidant capacity, in correlation with the method of extraction. For this purpose, Rheum ribes extraction was realized with four solvents of different polarities (50% methanol, 70% ethanol, 80% acetonitrile, and petroleum ether). We used different extraction techniques, such as orbital shaker, ultrasonic stirrer, microwave, and Soxhlet extraction, and the total phenolic content of the Rheum ribes extracts was determined by modified Folin–Ciocalteu method. The reducing power and radical scavenging activity of the extracts were also evaluated. The results shown that the antioxidant activity of the extracts depends on the extraction methods especially through the used solvent and decreases in the order: ethanol > methanol > acetonitrile > petroleum ether.

Influence of extraction method on antioxidant properties of <italic>Rheum ribes</italic> root extract

Faculty of Pharmacy, Department of Analytical Chemistry

Ovidius University Annals of Chemistry

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of extraction method on antioxidant properties of  root extract

Rheum species are important medicinal herbs, often used in pharmacological research, due to the presence of anthracene derivatives in the subterranean parts of...

Influence of extraction method on antioxidant properties of Rheum ribes root extract

Published Online: May 11, 2019

Page range: 44 - 47

Received: Feb 06, 2019

Accepted: Apr 25, 2019

DOI: https://doi.org/10.2478/auoc-2019-0008

Keywords
antioxidant activity, Folin–Ciocalteu assay, DPPH, FRAP

© 2019 Dilek Bilgic Alkaya et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of extraction method on antioxidant properties of Rheum ribes root extract

Published Online: May 11, 2019

Page range: 44 - 47

Received: Feb 06, 2019

Accepted: Apr 25, 2019

DOI: https://doi.org/10.2478/auoc-2019-0008

Keywordsantioxidant activity, Folin–Ciocalteu assay, DPPH, FRAP

© 2019 Dilek Bilgic Alkaya et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
antioxidant activity, Folin–Ciocalteu assay, DPPH, FRAP