<abstract xmlns="http://www.w3.org/1999/xhtml">Microbes play significant roles in remediation of heavy metal polluted industrial effluent using the mechanisms of biosorption and bioaccumulation. In the present study, six heavy metal resistant autochthonous bacteria species namely <italic>Bacillus cereus</italic>, <italic>B. megaterium</italic>, <italic>B. subtilis</italic>, <italic>Flavobacterium aquatile, Pseudomonas flourescens and Pseudomonas putida</italic> were isolated from effluent samples collected from Paper-mill industry (PMI), Paints and Chemicals Industry (PCI), and Steel-rolling Industry (SRI). The isolates were studied for their heavy metal tolerant capacities at different aqueous salt concentrations. Elemental analysis of the industrial effluent samples collected indicated the presence of heavy metals such as Copper (Cu2+), Manganese (Mn2+), Iron (Fe2+) and Lead (Pb2+) at varying concentrations in μg/ml. Generally, there were variations in the minimum inhibitory concentrations (MIC) of the heavy metal salt to each of the bacteria understudy. The MIC value of each of the bacterial isolates to aqueous solution of Cu2SO4 showed that <italic>B. megaterium</italic>, <italic>B. subtilis, Pseudomonas flourescens</italic> and <italic>Pseudomonas putida</italic> had the same MIC value of 20 ± 1.5 μg/mL while <italic>Bacillus cereus</italic> and <italic>Flavobacterium aquatile</italic> had MIC values of 13 ± 1.3 μg/mL and 25 ± 2.1 μg/mL respectively. This variation was also noticeable in aqueous salts of Mn2SO4, Fe2SO4 and Pb2SO4.The bacteria isolates showed sensitivity to heavy metals with increasing zone of inhibition as concentration increased with each isolate showing varying degree of metalotolerance. The effectiveness of the autochthonous bacteria as a means to bio-augment the remediation of heavy metal polluted industrial effluent was further proven and recommended.</abstract>

Microbes play significant roles in remediation of heavy metal polluted industrial effluent using the mechanisms of biosorption and bioaccumulation. In the present study, six heavy metal resistant autochthonous bacteria species namely Bacillus cereus, B. megaterium, B. subtilis, Flavobacterium aquatile, Pseudomonas flourescens and Pseudomonas putida were isolated from effluent samples collected from Paper-mill industry (PMI), Paints and Chemicals Industry (PCI), and Steel-rolling Industry (SRI). The isolates were studied for their heavy metal tolerant capacities at different aqueous salt concentrations. Elemental analysis of the industrial effluent samples collected indicated the presence of heavy metals such as Copper (Cu2+), Manganese (Mn2+), Iron (Fe2+) and Lead (Pb2+) at varying concentrations in μg/ml. Generally, there were variations in the minimum inhibitory concentrations (MIC) of the heavy metal salt to each of the bacteria understudy. The MIC value of each of the bacterial isolates to aqueous solution of Cu2SO4 showed that B. megaterium, B. subtilis, Pseudomonas flourescens and Pseudomonas putida had the same MIC value of 20 ± 1.5 μg/mL while Bacillus cereus and Flavobacterium aquatile had MIC values of 13 ± 1.3 μg/mL and 25 ± 2.1 μg/mL respectively. This variation was also noticeable in aqueous salts of Mn2SO4, Fe2SO4 and Pb2SO4.The bacteria isolates showed sensitivity to heavy metals with increasing zone of inhibition as concentration increased with each isolate showing varying degree of metalotolerance. The effectiveness of the autochthonous bacteria as a means to bio-augment the remediation of heavy metal polluted industrial effluent was further proven and recommended.

Metalotolerance Capacity of Autochthonous Bacteria Isolated From Industrial Waste Effluent

Microbiology Unit, Department of Pure and Applied Biology

Annals of Science and Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Microbes play significant roles in remediation of heavy metal polluted industrial effluent using the mechanisms of biosorption and bioaccumulation. In the...

Metalotolerance Capacity of Autochthonous Bacteria Isolated From Industrial Waste Effluent

Published Online: Apr 16, 2018

Page range: 13 - 17

Received: Feb 05, 2017

Accepted: Dec 29, 2017

DOI: https://doi.org/10.2478/ast-2018-0003

Keywords
Industrial effluent, Autochthonous bacteria, Metalotolerance, Waste

© 2018 Olusola N. Majolagbe et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Metalotolerance Capacity of Autochthonous Bacteria Isolated From Industrial Waste Effluent

Published Online: Apr 16, 2018

Page range: 13 - 17

Received: Feb 05, 2017

Accepted: Dec 29, 2017

DOI: https://doi.org/10.2478/ast-2018-0003

KeywordsIndustrial effluent, Autochthonous bacteria, Metalotolerance, Waste

© 2018 Olusola N. Majolagbe et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Industrial effluent, Autochthonous bacteria, Metalotolerance, Waste