<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Interactions between pentacyclic triterpenes isolated from white birch (Betula pendula Roth.) bark samples from Northeast Bulgaria and bovine serum albumin or human serum albumin were investigated using fluorescence techniques. The experimental results show the formation of complexes between the isolated triterpenes with serum albumins. Quenching of the intrinsic fluorescence of human serum albumins was monitored by emission spectra of varied quencher concentration solutions. By analysing the fluorescence spectra and fluorescence intensity, some parameters of the serum albumins - quencher interaction were determined to evaluate the type of quenching. An extract containing the isolated triterpenes formed complexes with both bovine serum albumin and human serum albumin, leading to quenching the fluorescence of both albumins by a combined quenching mechanism.</p>
</abstract>

Interactions between pentacyclic triterpenes isolated from white birch (Betula pendula Roth.) bark samples from Northeast Bulgaria and bovine serum albumin or human serum albumin were investigated using fluorescence techniques. The experimental results show the formation of complexes between the isolated triterpenes with serum albumins. Quenching of the intrinsic fluorescence of human serum albumins was monitored by emission spectra of varied quencher concentration solutions. By analysing the fluorescence spectra and fluorescence intensity, some parameters of the serum albumins - quencher interaction were determined to evaluate the type of quenching. An extract containing the isolated triterpenes formed complexes with both bovine serum albumin and human serum albumin, leading to quenching the fluorescence of both albumins by a combined quenching mechanism.

Interaction of the birch-bark terpenoids with human and bovine serum albumins

University of Shumen, Faculty of Natural Sciences

Vasil Levski National Military University

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution 3.0 Public License.

{"article-title":"Interaction of the birch-bark terpenoids with human and bovine serum albumins"}

Interactions between pentacyclic triterpenes isolated from white birch (Betula pendula Roth.) bark samples from Northeast Bulgaria and bovine serum albumin or...

Interaction of the birch-bark terpenoids with human and bovine serum albumins

Published Online: Nov 01, 2022

Page range: 25 - 35

DOI: https://doi.org/10.2478/asn-2022-0019

Keywords
Fluorescence quenching, Bovine serum albumin, Human serum albumin, triterpenoids

© 2022 Karamfil Kalchev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 3.0 Public License.

Interaction of the birch-bark terpenoids with human and bovine serum albumins

Published Online: Nov 01, 2022

Page range: 25 - 35

DOI: https://doi.org/10.2478/asn-2022-0019

KeywordsFluorescence quenching, Bovine serum albumin, Human serum albumin, triterpenoids

© 2022 Karamfil Kalchev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 3.0 Public License.

Keywords
Fluorescence quenching, Bovine serum albumin, Human serum albumin, triterpenoids