<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Phytochemical investigation of medicinally important plants has gained great attention, lately. This study aims to investigate the phytochemical constituents of the partitioned extracts (ethyl acetate (AI), dichloromethane (CI) and n-butanol (BI)) derived from the 70% ethanol extract of the rhizomes (RI) of Iris unguicularis Poir. an Algerian-Tunisian endemic plant and to evaluate their biological activities. Seventy constituents of these partitioned extracts were qualitatively characterized by liquid chromatography ion trap time of flight mass spectrometry (LC-MS-IT-TOF) technique. Moreover, quantitative screening of phenolic acids and flavonoids in the studied extracts was accomplished by a previously validated LC-MS/MS method. It was determined that, AI and CI, which were the richest extracts in terms of phytochemical content, showed high antioxidant and antibacterial activities in parallel with their identified constituents, while AI extract was rich in protocatechuic (1080.4 µg analyte/g), 4-OH-benzoic (2218.2 µg/g), syringic (1218.6 µg/g) and pcoumaric (1071.2 µg/g) acids, CI extract has been found to be rich in naringenin (1453.4 µg/g) and ferulic acid (1088.4 µg/g). These results revealed and confirmed the importance of the species and especially the genus rhizomes which have largely been used in folk medicine for the treatment of many diseases. However, all extracts of this endemic plant were inactive at all concentration against acetyl-and butyrylcholinesterase enzymes.</p></abstract>

Phytochemical investigation of medicinally important plants has gained great attention, lately. This study aims to investigate the phytochemical constituents of the partitioned extracts (ethyl acetate (AI), dichloromethane (CI) and n-butanol (BI)) derived from the 70% ethanol extract of the rhizomes (RI) of Iris unguicularis Poir. an Algerian-Tunisian endemic plant and to evaluate their biological activities. Seventy constituents of these partitioned extracts were qualitatively characterized by liquid chromatography ion trap time of flight mass spectrometry (LC-MS-IT-TOF) technique. Moreover, quantitative screening of phenolic acids and flavonoids in the studied extracts was accomplished by a previously validated LC-MS/MS method. It was determined that, AI and CI, which were the richest extracts in terms of phytochemical content, showed high antioxidant and antibacterial activities in parallel with their identified constituents, while AI extract was rich in protocatechuic (1080.4 µg analyte/g), 4-OH-benzoic (2218.2 µg/g), syringic (1218.6 µg/g) and pcoumaric (1071.2 µg/g) acids, CI extract has been found to be rich in naringenin (1453.4 µg/g) and ferulic acid (1088.4 µg/g). These results revealed and confirmed the importance of the species and especially the genus rhizomes which have largely been used in folk medicine for the treatment of many diseases. However, all extracts of this endemic plant were inactive at all concentration against acetyl-and butyrylcholinesterase enzymes.

Phytochemical profiles of <italic>Iris unguicularis</italic> Poir. with antioxidant, antibacterial, and anti-Alzheimer activities

Laboratory of Genetics Biochemistry and Plant Biotechnology, Faculty of Sciences of Nature and Life

University Frères Mentouri Constantine 1

Dicle University Science and Technology Research and Application Center (DUBTAM), Dicle University

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phytochemical profiles of  Poir. with antioxidant, antibacterial, and anti-Alzheimer activities

Phytochemical investigation of medicinally important plants has gained great attention, lately. This study aims to investigate the phytochemical constituents...

Phytochemical profiles of Iris unguicularis Poir. with antioxidant, antibacterial, and anti-Alzheimer activities

Published Online: Aug 10, 2020

Page range: 74 - 87

DOI: https://doi.org/10.2478/asn-2020-0021

Keywords
Poir., rhizomes, phenolic compounds, biological activities, LC-MS/MS, LCMS-IT-TOF

© 2020 Souheir Bensari et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phytochemical profiles of Iris unguicularis Poir. with antioxidant, antibacterial, and anti-Alzheimer activities

Published Online: Aug 10, 2020

Page range: 74 - 87

DOI: https://doi.org/10.2478/asn-2020-0021

KeywordsPoir., rhizomes, phenolic compounds, biological activities, LC-MS/MS, LCMS-IT-TOF

© 2020 Souheir Bensari et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Poir., rhizomes, phenolic compounds, biological activities, LC-MS/MS, LCMS-IT-TOF