1. bookVolume 7 (2020): Issue 2 (July 2020)
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Evaluation of phenolic contents and antioxidant activities of some medicinal plants growing in Algerian Aurès Mountains

Published Online: 10 Aug 2020
Page range: 15 - 30
Journal Details
License
Format
Journal
First Published
15 Dec 2015
Publication timeframe
1 time per year
Languages
English
Copyright
© 2020 Sciendo

The objective of this study is to evaluate the antioxidant activities of six medicinal plants growing in Algerian Aurès Mountains. Total phenolic and flavonoids contents were measured using colorimetric methods, and the antioxidant capacities were evaluated using the DPPH radical scavenging and β-carotene bleaching tests. Juniperus phoenica L. had significantly the higher total phenolic compounds (53.6±3.86 mg GAE.g−1 DM) (p<0.05); followed by Romarinus officinalis L. (26.1±3.15 mg GAE.g−1 DM) and Artemisia campestris L. (20.5±1.99 mg GAE.g−1 DM). Artemisia campestris L. had significantly the higher flavonoid contents (11.1±0.56 mg QE.g−1 DM) than other studied plants (p<0.05). The best antiradical activity was observed in Thymus algeriensis extracts (EC50=11.1±0.33 µg.ml−1) and Romarinus officinalis L. (EC50=15.3±0.9 µg.ml−1). β-carotene bleaching test showed that the herbs’ phenolic compounds Antioxidant Activity (AA%) value was found in the range of 64-84%, whereas that of the standard antioxidant ascorbic acid was 51±2.4%. The present results indicate that medicinal plants from the Algerian Aurès mountains could be explored in food and pharmaceutical industries for development of natural’s antioxidant agents.

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[1] Boutellaa, S.; Zellagui, A.; Öztürk, M.; Bensouici, C.; Ölmez, Ö. T.; Menakh, M.; Duru, M. E. HPLCDAD profiling and antioxidant activity of the n-butanol extract from aerial parts of Algerian Crithmum maritimum L. Acta Sci. Nat. 2019, 6, 8-16.Search in Google Scholar

[2] Ergül, M.; Ergül, M.; Eruygur, N.; Atas, M.; Uçar, E. In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turk J Pharm Sci. 2019, 16, 401-409.Search in Google Scholar

[3] Boulacel, I.; Harkati, B.; Ayad, R.; Demirtas, I.; Laouer, H.; Akkal, S. Phytochemical Studies Antibacterial and Antioxidant Activities of Aerial Parts of Ferula lutea (Poir.) Maire. Acta Sci. Nat. 2019, 6, 17-25.Search in Google Scholar

[4] Karatoprak, G.Ş.; Ilgün, S.; Koşar, M. Original article Antioxidant Properties and Phenolic Composition of Salvia. Turk J Pharm Sci. 2016, 13(2), 201-212.Search in Google Scholar

[5] Chohra, D.; Ferchichi, L. Ethnobotanical study of Belezma National Park (BNP) plants in Batna: East of Algeria. Acta Sci. Nat. 2019, 6, 40–54.Search in Google Scholar

[6] Tlili, N.; Elfalleh, W.; Hannachi, H.; Yahia, Y.; Khaldi, A.; Ferchichi, A.; Nasri, N. Screening of Natural Antioxidants from Selected Medicinal Plants. Int J Food Prop. 2013, 16, 1117-1126.Search in Google Scholar

[7] Ibrahim, M. A.; Ramadan, M.M. Cytotoxic effects on MCF-7 breast cancer cell lines, phenol and flavonoid contents, high performance liquid chromatography (HPLC) analysis and antioxidant activity of Maerua pseudopetalosa (Gilg and Bened) De Wolf fractions. African J Pharm Pharmacol. 2019, 13, 314-321.Search in Google Scholar

[8] Ouelbani, R.; Bensari, S.; Mouas, T. N.; Khelifi, D. Ethnobotanical investigations on plants used in folk medicine in the regions of Constantine and Mila (North-East of Algeria). J Ethnopharmacol. 2016, 194, 196-218.Search in Google Scholar

[9] Boudjelal, A.; Henchiri, C.; Sari, M.; Sarri, D.; Hendel, N.; Benkhaled, A.; Ruberto, G. Herbalists and wild medicinal plants in M’Sila (North Algeria): An ethnopharmacology survey. J Ethnopharmacol. 2013, 148, 395-402.Search in Google Scholar

[10] Bouasla, A.; Bouasla, I. Ethnobotanical survey of medicinal plants in northeastern of Algeria. Phytomedicine. 2017, 36, 68-81.Search in Google Scholar

[11] Benarba, B. Medicinal plants used by traditional healers from South-West Algeria: An ethnobotanical study. J Intercult Ethnopharmacol. 2016, 5, 320-330.Search in Google Scholar

[12] Chermat, S.; Gharzouli, R. Ethnobotanical Study of Medicinal Flora in the North East of Algeria - An Empirical Knowledge in Djebel Zdimm (Setif). J Mater Sci Eng A. 2015, 5, 50-59.Search in Google Scholar

[13] Ramdane, F.; Mahfoud, H. M.; Mohamed, D. O. H.; Roukia, H.; Naima, H.; Houria, M.; Bouafia, I.; Bahaz, C. Ethnobotanical study of some medicinal plants from Hoggar, Algeria. J Med Plants Res. 2015, 9, 820-827.Search in Google Scholar

[14] Falleh, H.; Ksouri, R.; Chaieb, K.; Karray-Bouraoui, N.; Trabelsi, N.; Boulaaba, M.; Abdelly, C. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. C R Biol. 2008, 331, 372-379.Search in Google Scholar

[15] Singleton, V. L.; Orthofer, R.; Lamuela-Raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in Enzymology, 1999, 299, 152-178.Search in Google Scholar

[16] Woisky, R. G.; Salatino, A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res. 1998, 37, 99-105.Search in Google Scholar

[17] Dung, N. T.; Kim, J. M.; Kang, S. C. Chemical composition, antimicrobial and antioxidant activities of the essential oil and the ethanol extract of Cleistocalyx operculatus (Roxb.) Merr and Perry buds. Food Chem Toxicol. 2008, 46, 3632-3639.Search in Google Scholar

[18] Rashid, A.; Qureshi, M. Z.; Raza, S. A.; William, J.; Arshad, M. Quantitative determination of antioxidant potential of Artemisia persica. Analele Univ din Bucureşti. 2010, 19, 23-30.Search in Google Scholar

[19] Gramza, A.; Pawlak-Lemańska, K.; Korczak, J.; Wąsowicz, E.; Rudzinska, M. Tea Extracts as Free Radical Scavengers. Polish J Environ Stud. 2005, 14, 861–867.Search in Google Scholar

[20] Kulisic, T.; Radonic, A.; Katalinic, V.; Milos, M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem. 2004, 85, 633-640.Search in Google Scholar

[21] Ismail, A.; Marjan, Z.; Foong, C. Total antioxidant activity and phenolic content in selected vegetables. Food Chem. 2004, 87, 581-586.Search in Google Scholar

[22] Djeridane, A.; Yousfi, M.; Nadjemi, B.; Boutassouna, D.; Stocker, P.; Vidal, N. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006, 97, 654-660.Search in Google Scholar

[23] Nikolić, M.; Glamočlija, J.; Ferreira, I.C.F.R.; Calhelha, R. C.; Fernandes, Â.; Marković, T.; Marković, D.; Giweli, A.; Soković, M. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Ind Crops Prod. 2014, 52, 183-190.Search in Google Scholar

[24] Katalinic, V.; Milos, M.; Kulisic, T.; Jukic, M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem. 2006, 94, 550-557.Search in Google Scholar

[25] Hayouni, E. A.; Abedrabba, M.; Bouix, M.; Hamdi, M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem. 2007, 105, 1126-1134.Search in Google Scholar

[26] Fadili, K.; Amalich, S.; N’dedianhoua, S. K.; Bouachrine, M.; Mahjoubi, M.; El Hilali, F.; Zair, T. Teneurs en polyphénols et évaluation de l’activité antioxydante des extraits de deux espèces du Haut Atlas du Maroc: Rosmarinus Officinalis et Thymus Satureioides. Int J Innov Sci Res ISSN. 2015, 17, 2351-8014.Search in Google Scholar

[27] Celiktas, O.Y.; Kocabas, E. E. H.; Sukan, F.V.; Bedir, E.; Ozek, T.; Baser, K. H. C. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem. 2005, 100, 553-559.Search in Google Scholar

[28] Khlifi, D.; Sghaier, R. M.; Amouri, S.; Laouini, D.; Hamdi, M.; Bouajila, J. Composition and anti-oxidant, anti-cancer and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. Food Chem Toxicol. 2013, 55, 202-208.Search in Google Scholar

[29] Zouaoui, N.; Chenchouni, H.; Bouguerra, A.; Massouras, T.; Barkat M. Characterization of volatile organic compounds from six aromatic and medicinal plant species growing wild in North African drylands. NFS J. 2020, 18, 19-28.Search in Google Scholar

[30] Megdiche-Ksouri, W.; Bourgou, S.; Snoussi, M. Artemisia campestris phenolic compounds have antioxidant and antimicrobial activity. Ind Crops Prod. 2014, 63, 104-113.Search in Google Scholar

[31] Ghlissi, Z.; Sayari, N.; Kallel, R.; Bougatef, A.; Sahnoun, Z. Antioxidant, antibacterial, anti-inflammatory and wound healing effects of Artemisia campestris aqueous extract in rat. Biomed Pharmacother. 2016, 84, 115-122.Search in Google Scholar

[32] Da Silva Port’s, P.; Chisté, R.C.; Godoy, H. T.; Prado, M.A. The phenolic compounds and the antioxidant potential of infusion of herbs from the Brazilian Amazonian region. Food Res Int. 2013, 53, 875-881.Search in Google Scholar

[33] Pukalskas, A.; Venskutonis, P.R.; Salido, S.; Waard, P. De.; Van Beek, T.A. Isolation, identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania. Food Chem. 2012, 130, 695-701.Search in Google Scholar

[34] Sbayou, H.; Boumaza, A.; Hilali, A.; Amghar, S. Antioxidant properties of Artemisia herba-alba Asso., Mentha pulegium L. and Origanum compactum Benth. essential oils. J Mater Environ Sci. 2016, 7, 2908-2912.Search in Google Scholar

[35] Cai, Y. Z.; Luo, Q.; Sun, M.; Corke, H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004, 74, 2157-2184.Search in Google Scholar

[36] Li, N.; Mao, Y.; Deng, C.; Zhang, X. Separation and identification of volatile constituents in Artemisia argyi flowers by GC-MS with SPME and steam distillation. J Chromatogr Sci.2008, 46, 401-405.Search in Google Scholar

[37] Jordán, M.J.; Moñino, M.I.; Martínez, C.; Lafuente, A.; Sotomayor, J.A. Introduction of distillate rosemary leaves into the diet of the Murciano-Granadina goat: Transfer of polyphenolic compounds to goats milk and the plasma of suckling goat kids. J Agric Food Chem. 2010, 58, 8265-8270.Search in Google Scholar

[38] Alu’datt, M.H.; Rababah, T.; Alhamad, M. N.; Al-Mahasneh, M. A.; Almajwal, A.; Gammoh, S.; Ereifej, K.; Johargy, A.; Alli, I. A review of phenolic compounds in oil-bearing plants: Distribution, identification and occurrence of phenolic compounds. Food Chem. 2017, 218, 99-106.Search in Google Scholar

[39] Djidel, S.; Khennouf, S. Radical scavenging, reducing power, lipid peroxidation inhibition and chelating properties of extracts from Artemisia campestris L. aerial parts. Annu Res Rev Biol. 2014, 4, 1691-1702.Search in Google Scholar

[40] Dib, I.; Angenot, L.; Mihamou, A.; Ziyyat, A.; Tits, M. Artemisia campestris L.: Ethnomedicinal, phytochemical and pharmacological review. J Herb Med. 2017, 7, 1-10.Search in Google Scholar

[41] Fukumoto, L.R.; Mazza, G. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem. 2000, 48, 3597-3604.Search in Google Scholar

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