<abstract xmlns="http://www.w3.org/1999/xhtml">During recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause cellular death by inducing oxidative stress. Present study aimed to investigate whether diferuloylmethane could protect rats mitochondria against Fe3O4-NPs intoxication. Twenty adult male rats were randomly chosen and divided into four groups: control; treated with 10 mg/kg/d of Fe3O4-NPs; treated with diferuloylmethane at the dose 20 ml/kg/d; treated with Fe3O4-NPs (10 mg/kg/d) and diferuloylmethane (20 ml/kg/d) respectively for 28 days. The results showed that Fe3O4-NPs increased the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation, mit-GSH (Glutathione), mit-CAT (Catalase), mit-GST (Glutathione S-transferase) and decreased mit-GPx (Glutathione peroxidase), with increased in mitochondrial swelling and permeability followed by the increasing level of plasmatic Cyt-c. The addition of diferuloylmethane (DFM) to these samples reduces or corrects the amount of the most of biomarkers. These findings have demonstrated that DFM can act as an antioxidant and antiapoptotic factor against damages induced by Fe3O4-NPs.</abstract>

During recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause cellular death by inducing oxidative stress. Present study aimed to investigate whether diferuloylmethane could protect rats mitochondria against Fe3O4-NPs intoxication. Twenty adult male rats were randomly chosen and divided into four groups: control; treated with 10 mg/kg/d of Fe3O4-NPs; treated with diferuloylmethane at the dose 20 ml/kg/d; treated with Fe3O4-NPs (10 mg/kg/d) and diferuloylmethane (20 ml/kg/d) respectively for 28 days. The results showed that Fe3O4-NPs increased the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation, mit-GSH (Glutathione), mit-CAT (Catalase), mit-GST (Glutathione S-transferase) and decreased mit-GPx (Glutathione peroxidase), with increased in mitochondrial swelling and permeability followed by the increasing level of plasmatic Cyt-c. The addition of diferuloylmethane (DFM) to these samples reduces or corrects the amount of the most of biomarkers. These findings have demonstrated that DFM can act as an antioxidant and antiapoptotic factor against damages induced by Fe3O4-NPs.

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe3o4)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Applied Biology department, Faculty of Exact, Nature and life sciences

Laboratory of Toxicology and Ecosystems pathologies

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Feo)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

During recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause...

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe₃o₄)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Published Online: May 06, 2020

Page range: 140 - 154

DOI: https://doi.org/10.2478/asn-2020-0014

Keywords
mitochondrial status, oxidative stress, Cyt-c, apoptosis, diferuloylmethane

© 2020 Zina Bouteraa et al, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe3o4)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Published Online: May 06, 2020

Page range: 140 - 154

DOI: https://doi.org/10.2478/asn-2020-0014

Keywordsmitochondrial status, oxidative stress, Cyt-c, apoptosis, diferuloylmethane

© 2020 Zina Bouteraa et al, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe₃o₄)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Keywords
mitochondrial status, oxidative stress, Cyt-c, apoptosis, diferuloylmethane