1. bookVolume 6 (2019): Issue 2 (December 2019)
Journal Details
First Published
15 Dec 2015
Publication timeframe
1 time per year
access type Open Access

Green-extraction of essential oil of the species Ruta chalepensis L.: gas chromatography-mass spectroscopy-infra red analysis and response surface methodology optimization

Published Online: 08 Nov 2019
Page range: 88 - 109
Journal Details
First Published
15 Dec 2015
Publication timeframe
1 time per year

The objective of the present study was the optimization of the parameters affecting the hydrodistillation of Ruta chalepensis L. essential oil using response surface design type Box-Behnken. After an appropriate choice of three parameters, 15 experiments were performed leading to a mathematical second-degree model relating the response function (yield of essential oil) to parameters and allowing a good control of the extraction process. The realization of the experiments and data analysis was carried out by response surface methodology (RSM). A deduced second-order polynomial expression was used to determine the optimal conditions necessary to obtain a better essential oil yield. These optimized operating conditions were: a granulometry of 2 mm, a condensation-water flow rate of 3.4 mL/min and an extraction time of 204 min. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2=0.9589 and P<0.006. The gas chromatography-mass spectrometry analysis of essential oil extracted from the Ruta chalepensis L. aerial parts revealed the presence of 2-undecanone, 2-nonanone and 2-decanone as major components.


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