<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>The first step of the proteomic study is the extraction and the success of this technique was based mainly on the choice of the best extraction. The purpose of this study was to determine the simplest and lowest-cost method of total protein extraction. Initially, various extraction methods were performed for protein extraction from roots of <italic>Astragalus armatus</italic> Willd. The methods employed were extraction by RIPA buffer, hypotonic buffer and distilled water. After determination of protein concentration by Bradford method and SDS PAGE electrophoretic analysis, the quantity and quality of extracted proteins using different protocols from <italic>A. armatus</italic> were determined and compared. The protein yield of RIPA buffer method was higher than the yields of hypotonic buffer method and distilled water method. The best protein patterns were produced by RIPA buffer method. The extract obtained by RIPA buffer was the optimal protocol for protein extraction.</p>
</abstract>

The first step of the proteomic study is the extraction and the success of this technique was based mainly on the choice of the best extraction. The purpose of this study was to determine the simplest and lowest-cost method of total protein extraction. Initially, various extraction methods were performed for protein extraction from roots of Astragalus armatus Willd. The methods employed were extraction by RIPA buffer, hypotonic buffer and distilled water. After determination of protein concentration by Bradford method and SDS PAGE electrophoretic analysis, the quantity and quality of extracted proteins using different protocols from A. armatus were determined and compared. The protein yield of RIPA buffer method was higher than the yields of hypotonic buffer method and distilled water method. The best protein patterns were produced by RIPA buffer method. The extract obtained by RIPA buffer was the optimal protocol for protein extraction.

An Efficient Protein Extraction Method from <italic>Astragalus armatus</italic> Willd. Roots for Proteomic Analysis

Department of Molecular and Cellular Biology, Faculty of Natural and Life Sciences

Advanced Research in Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

An Efficient Protein Extraction Method from  Willd. Roots for Proteomic Analysis

The first step of the proteomic study is the extraction and the success of this technique was based mainly on the choice of the best extraction. The purpose of...

An Efficient Protein Extraction Method from Astragalus armatus Willd. Roots for Proteomic Analysis

Published Online: Mar 16, 2024

Page range: 34 - 38

Received: Nov 01, 2023

Accepted: Jan 01, 2024

DOI: https://doi.org/10.2478/arls-2024-0004

Keywords
RIPA buffer, Hypotonic buffer, distilled water, SDS PAGE

© 2024 Messai Alima et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

An Efficient Protein Extraction Method from Astragalus armatus Willd. Roots for Proteomic Analysis

Published Online: Mar 16, 2024

Page range: 34 - 38

Received: Nov 01, 2023

Accepted: Jan 01, 2024

DOI: https://doi.org/10.2478/arls-2024-0004

KeywordsRIPA buffer, Hypotonic buffer, distilled water, SDS PAGE

© 2024 Messai Alima et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
RIPA buffer, Hypotonic buffer, distilled water, SDS PAGE