1. bookVolume 5 (2021): Issue 1 (January 2021)
Journal Details
License
Format
Journal
First Published
16 Jun 2017
Publication timeframe
1 time per year
Languages
English
access type Open Access

Splenic and Leucocytic Responses in Wistar Rats Exposed to Chronic Doses of Hydromethanol Extract of Helianthus annuus Leaf in Feed

Published Online: 12 Apr 2021
Page range: 12 - 17
Received: 01 Sep 2020
Accepted: 01 Mar 2021
Journal Details
License
Format
Journal
First Published
16 Jun 2017
Publication timeframe
1 time per year
Languages
English
Abstract

To evaluate the effects of the hydromethanol extract of Helianthus annuus on leucocyte profile and spleen histology after prolonged exposure to Wistar rats. This study involved the incorporation of varied concentrations (2.50, 5.00 and 10.00 mg/10 g) of hydromethanol extract of H. annuus in feed and feeding same to Wistar rats for 90 consecutive days. Blood samples were collected from the retro-orbital plexus of the rats on days 30, 60 and 90, for leucocyte count. Histopathological examination of the spleen was also conducted. The extract treatment did not cause a significant (p >0.05) change in the leucocyte profile and the spleen histology in the treated rats when compared to the normal control rats. On day 90, the total leucocyte counts of 15.24 ± 1.47, 12.69 ± 0.84 and 16.29 ± 3.36 for the groups that were treated with extract 2.50, 5.00 and 10.00 mg/10 g feed respectively, were not significantly (p > 0.05) different when compared with the total leucocyte count (12.01 ± 0.77) of the control group. The findings suggest that hydromethanol extract of H. annuus do not have a significant effect on the leucocyte profile and the histology of spleen.

Keywords

1. Kaid, F., Alabsi, A.M., Alafifi, N., Ali-Saeed, R., Ameen, A.M., Ramanathan, A. & Ali, A.M. (2019). Histological, biochemical, and hematological effects of Goniothalamin on selective internal organs of male Sprague-Dawley rats. Journal of Toxicology. https://doi.org/10.1155/2019/6493286 Search in Google Scholar

2. World Health Organization (2000). General guidelines for methodologies on research and evaluation of traditional medicine. World Health Organization. Retrieved July, 20, 2020, from https://apps.who.int/iris/bitstream/handle/10665/66783/WHO_EDM_TRM_2000.1.pdf Search in Google Scholar

3. World Health Organization. (2002). Traditional medicine strategy 2002-2005. Geneva: World Health Organization. Search in Google Scholar

4. Habtom, G.K. (2018). Perceptions and attitudes of modern and traditional medical practitioners about traditional medical practice in Eritrea. International Journal of Complementary and Alternative Medicine, 11 (1), 6-19. DOI: 10.15406/ijcam.2018.11.00340 Search in Google Scholar

5. Sen, S. & Chakraborty, R. (2017). Revival, modernization and integration of Indian traditional herbal medicine in clinical practice: Importance, challenges and future. Journal of Traditional and Complementary Medicine, 7 (2), 234-44. DOI: 10.1016/j.jtcme.2016.05.006 Search in Google Scholar

6. Onoja, S.O., Udem, S.C., Anaga, A.O. & Asuzu, I.U. (2018). Acute and chronic toxicity studies of hydromethanol leaf extract of Helianthus annuus Linn. in rats. Asian Pacific Journal of Tropical Medicine, 11(9), 534-539. https://www.apjtm.org/text.asp?2018/11/9/534/242311 Search in Google Scholar

7. Mensah, M.L., Komlaga, G., Forkuo, A.D., Firempong, C., Anning, A.K. & Dickson, R.A. (2019). Toxicity and safety implications of herbal medicines used in Africa, Herbal Medicine, Philip F. Builders, IntechOpen. Search in Google Scholar

8. Debelo, N., Afework, M., Debella, A., Makonnen, E., Ergete, W. & Geleta, B. (2016). Assessment of hematological, biochemical and histopathological effects of acute and sub-chronic administration of the aqueous leaves extract of Thymus schimperi in rats. Journal of Clinical Toxicology, 6 (286), 2161-0495. Search in Google Scholar

9. Kale, O.E., Awodele, O. & Akindele, A.J. (2019). Subacute and subchronic oral toxicity assessments of Acridocarpus smeathmannii (DC.) Guill. & Perr. root in Wistar rats. Toxicology Reports, 6, 161-75. Search in Google Scholar

10. Olayode, O.A., Daniyan, M.O. & Olayiwola, G. (2019). Biochemical, hematological and histopathological evaluation of the toxicity potential of the leaf extract of Stachytarpheta cayennensis in rats. Journal of Traditional and Complementary Medicine, https://doi.org/10.1016/j.jtcme.2019.05.001 Search in Google Scholar

11. Banerjee, B.D. (1999). The influence of various factors on immune toxicity assessment of pesticide chemicals. Toxicology Letters, 107 (1-3), 21-31. Search in Google Scholar

12. Gessner, D.K., Ringseis, R. & Eder, K. (2017). Potential of plant polyphenols to combat oxidative stress and inflammatory processes in farm animals. Journal of Animal Physiology and Animal Nutrition, 101 (4), 605-628. Search in Google Scholar

13. Hopkins, A., Rowland, A., Kichenadasse, G., Wiese, M.D., Gurney, H., McKinnon, R.A., Karapetis, C.S. & Sorich, M.J. (2017). Predicting response and toxicity to immune checkpoint inhibitors using routinely available blood and clinical markers. British Journal of Cancer, 117, 913–920. doi: 10.1038/bjc.2017.274 Search in Google Scholar

14. Lewis, E.D., Goruk, S., Richard, C., Dellschaft, N.S., Curtis, J.M., Jacobs, R.L. & Field, C.J. (2016). Feeding a diet devoid of choline to lactating rodents restricts growth and lymphocyte development in offspring. Bristish Journal of Nutrition, 116 (6), 1001-1012. DOI: 10.1017/S0007114516002919 Search in Google Scholar

15. Onoja, S.O., Udem, S.C. & Anaga, A.O. (2017). Effect of chronic administration of hydromethanol leaf extract of Helianthus annuus on erythrocytic profile in normal rats. Journal of Nutritional Therapy, 6 (2), 59–63. Search in Google Scholar

16. Ejebe, D.E., Emudainohwo, J.O., Akonoghrere, R., Olise, C.C., Amadi, C.N. & Siminialayi, I.M. (2008). Effects of ethanol extract of leaves of Helianthus annuus on the reproductive system of male wistar rats: Testicular histology, epididymal sperm properties and blood levels of reproductive hormones. Biomedical and Pharmacology Journal, 1 (1), 65-78. Search in Google Scholar

17. Onoja, S.O. & Anaga, A.O. (2014). Evaluation of the antidiabetic and antioxidant potentials of methanolic leaf extract of Helianthus annuus L. on alloxan-induced hyperglycemic rats. Comparative Clinical Pathology, 23, 1565-1573. Search in Google Scholar

18. Brar, R.S., Sandhu, H.S. & Singh, A. (2000). Veterinary clinical diagnosis by laboratory methods. New Delhi: Kalyani Publishers. Search in Google Scholar

19. Ezeja, M.I., Anaga, A.O. & Asuzu, I.U. (2014). Acute and sub-chronic toxicity profile of methanol leaf extract of Gouania longipetala in rats. Journal of Ethnopharmacology, 151 (3), 1155–1164 Search in Google Scholar

20. Craig, M.E., Jefferies, C., Dabelea, D., Balde, N., Seth, A. & Donaghue, K.C. (2014) Definition, epidemiology, and classification of diabetes in children and adolescents. Pediatrics Diabetes, 15(Suppl 20), 4–17. Search in Google Scholar

21. Ferreira, I.C.F.R., Martins, N. & Barros, L. (2017). Phenolic compounds and its bioavailability: In vitro bioactive compounds or health promoters? Advances in Food and Nutrition Research, 82, 1–44. Search in Google Scholar

22. Campbell, T.W. (2012) Mammalian Hematology: Laboratory Animals and Miscellaneous Species. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds) Veterinary hematology and clinical chemistry, 2nd edn. Wiley-Blackwell, Hoboken, pp 225–237. Search in Google Scholar

23. Das, A. & Mohanty, P.K. (2018). Comparative hemocytobiochemical profile of Rattus rattus Linnaeus, 1758 and Rattus norvegicus Berkenhout, 1769. Comparative Clinical Pathology, 27, 1509–1514. Search in Google Scholar

24. Ajao, M.S., Sansa, A.B., Imam, A., Ibrahim, A., Adana, M.Y., Alli-Oluwafuyi, A. & Kareem, S.B. (2017). Protective Effect of Nigella Sativa (Black Caraway (Oil on Oral Dichlorvos Induced Hematological, Renal and Nonspecific Immune System Toxicity in Wistar Rats. Iranian Journal of Toxicology, 11 (6), 1-5. Search in Google Scholar

25. Widiastuti, E.L., Arifianti, R., Khairani, I.A., Christianto, Y., Ara, N.F. & Maharani. H.W. (2019). Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo (α) pyrene. IOP Conference Series: Earth and Environmental Science, 305, 012011. doi:10.1088/1755-1315/305/1/012011 Search in Google Scholar

26. Akpovona, A. & Onoagbe, I. (2018). Investigation of haematological effects of Terminalia macroptera stem bark via platelet-to-lymphocyte ratio among other blood parameters in Wistar rats. Biokemistri, 30 (1), 32-41. Search in Google Scholar

27. Drummond, G.B. (2009). Reporting ethical matters in The Journal of Physiology: standards and advice. Journal of Physiology, 587 (4), 713-719. Search in Google Scholar

28. Richter, F., Fehske, A.J. & Fettweis, G.P. (2009) Energy efficiency aspects of base station deployment strategies for cellular networks. In Vehicular Technology Conference Fall (VTC 2009-Fall), IEEE 70th (pp. 1-5). IEEE. Search in Google Scholar

29. Davis, A.K., Maney, D.L. & Maerz, J.C. (2008). The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology, 22 (5), 760-772. Search in Google Scholar

30. Emamuzo, E.D., Miniakiri, S.I., Tedwin, E.J., Ufouma, O. & Lucky, M. (2010). Analgesic and anti— inflammatory activities of the ethanol extract of the leaves of Helianthus Annus in Wistar rats. Asian Pacific Journal of Tropical Medicine, 3 (5), 341-347. Search in Google Scholar

31. He, S.H., Zhang, H.Y., Zeng, X.N., Chen, D. & Yang, P.C. (2013). Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis. Acta Pharmacologica Sinica, 34 (10): 1270-1283. Search in Google Scholar

32. Cesta, M.F. (2006). Normal structure, function, and histology of the spleen. Toxicologic Pathology, 34 (5), 455-465. Search in Google Scholar

33. Mebius, R.E. & Kraal, G. (2005). Structure and function of the spleen. Nature Reviews Immunology, 5 (8), 606-616. Search in Google Scholar

34. Lewis, S.M., Williams, A. & Eisenbarth, S.C. (2019). Structure and function of the immune system in the spleen. Science Immunology, 4 (33), eaau6085. doi: 10.1126/sciimmunol.aau6085. Search in Google Scholar

35. Seifert, H.A. & Offner, H. (2018). The splenic response to stroke: from rodents to stroke subjects. Journal of Neuroinflammation, 15 (1), 195. https://doi.org/10.1186/s12974-018-1239-9 Search in Google Scholar

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