1. bookVolume 3 (2019): Issue 1 (April 2019)
Journal Details
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16 Jun 2017
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English
access type Open Access

Mycoflora of Maize in Niger State, Nigeria

Published Online: 25 Jun 2019
Page range: 40 - 45
Accepted: 01 Jun 2019
Journal Details
License
Format
Journal
First Published
16 Jun 2017
Publication timeframe
1 time per year
Languages
English

Fungi have been associated with contamination and spoilage of food. These organisms are known to thrive in different weather and environmental conditions. Maize kernel obtained from field, store and market from 25 Local Government Areas of Niger State within the month of August to September were analyzed for fungi occurrence using standard methods. Ten different fungus; Aspergillus niger, A. ochraceus, A. flavus, A. fumigatus, A. parasiticus, Mucor spp, Fusarium spp, Penicillium spp, Yeast and Rhizopus spp, were identified and isolated from the maize kernel. From the agro-ecological zones of the state, the most predominant fungi were Aspergillus species (241/345), Rhizopus spp (41/345) and Mucor spp (37/345) while Penicilium spp and yeast were the least identified. Within the zones, Aspergillus species have the highest occurrence; wet (65/95), driest (63/95), wettest (56/95) and dry zone (54/95) zones. The highest occurrence of fungi was found in the market sample (129) followed by store (112) and field (104). High percentage occurrence of 11.6% was recorded in Suleja, Wushishi, and Borgu Local Government Area, followed by 10.6, 10.1, 8.7, 9.7, 9.2, 8.7 and 8.2% in Bosso, Tafa, Madalla, Minna, Paiko, Bida and Sabon wuse respectively. Mold species known to be mycotoxin producers were reported in this work. This poses as a risk factor to food safety.

Keywords

1. CAST (Council for Agricultural Science and Technology) (2003). Mycotoxins - risks in plant, animal and human systems, Task Force Report, No. 139, Ames, Iowa. pp1-191.Search in Google Scholar

2. Bhat RV, Vasanthi S. (2003). Food safety in food security and food trade. Mycotoxin Food Safety Risk in Developing Countries IFPRI. Brief.Search in Google Scholar

3. FAOSTAT: FAO Statistical Database, Food and Agricultural Organization of the United Nations, Rome, Italy, available at: http://faostat.fao.org/, last access: 18 December 2016.Search in Google Scholar

4. Makun HA, Apeh DO, Rinde Y, Nagogo T, Okeke JO, Mustapha AS, et al.,. (2014). Determination of Aflatoxins in Sesame, Rice, Millet and Acha from Nigeria Using HPLC. Chemical Science Transactions; 3: 1516-1524.Search in Google Scholar

5. Coulibaly O, Kerstin H, Bandyopadhyay R, Hounkponou S, Leslie JF, (2008). Economic impact of aflatoxin contamination in sub-Saharan Africa. In: Leslie, J.F., Bandyopadyay, R., Visconti, A. (Eds.), Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade. CAB International, pp. 67-76.Search in Google Scholar

6. Dawlal P, Barros E, Marais GJ, (2012). Evaluation of maize cultivars for their susceptibility towards mycotoxigenic fungi under storage conditions. Journal of stored products research, 48, 114-119. https://doi.org/10.1016/j.jspr.2011.10.00610.1016/j.jspr.2011.10.006Open DOISearch in Google Scholar

7. Wilson DM, Abramson D, (1992). Mycotoxins. In: Sauer, D.B. (Ed.), Storage of Cereal Grains and their Products, fourth ed. American Association of Cereal Chemists, pp. 341-391 (chapter 10), 615.Search in Google Scholar

8. Bailey JE. (1992). Whole grain storage. In: Sauer, D.B. (Ed.), Storage of Cereals Grains and their Products, fourth ed. American Association of Cereal Chemists, pp. 157-187 (chapter 5), 615.Search in Google Scholar

9. Adebajo LO, Idowu AA, Adesanya OO. (1994). Mycoflora and mycotoxins production in Nigerian corn and corn-based snacks. Mycopathologia 126: 183–192.Search in Google Scholar

10. Makun HA, Gbodi TA, Akanya OH, Salako AE, Ogbadu GH (2009). Health implications of toxigenic fungi found in two Nigerian staples: guinea corn and rice. African Journal of Food and Science 3: 250-256.Search in Google Scholar

11. Opadokun JS, Ikeorah JN, Afolabi E, (1979). The aflatoxin contents of locally consumed foodstuffs, Part 3: Maize, Nigerian Stored Product Research Institute Report; NSPRI, Ilorin, Nigeria, pp. 105-108.Search in Google Scholar

12. Lillehoj EB (1991). Aflatoxin: an ecologically elicited activation signal. In: Smith, J.E., Anderson, R.A. (Eds.), Mycotoxins and Animal Foods. CRC Press, Boca Raton, FL, pp. 119–139.Search in Google Scholar

13. Adesuyi SA, (1972). The problems of storage of local foodstuffs in southern Nigeria. In: Annual Conference of the Nigerian Society of Plant Protection.Search in Google Scholar

14. Okereke GU, Nwosu VC. (1987). Crop storage losses in southern Nigeria caused by the activities of microorganisms. MIRCEN. Journal of Microbiology and Biotechnology 3: 201-210.Search in Google Scholar

15. Okoye ZS, (1993). Fusarium mycotoxins nivalenol and 4-acetyl-nivalenol (fusarenon-X) in mouldy maize harvested from farms in Jos district, Nigeria. Food Additives and Contaminants 10: 375–379.Search in Google Scholar

16. Hell K, Udoh J, Setamou M, Cardwell, KF, Visconti A (1996). Fungal Infection and Mycotoxins in Maize in the Different Agro-ecological Zones of Benin and Nigeria. Food and Chemical Toxicology 4: 11-16.Search in Google Scholar

17. Udoh JM, Cardwell KF, Ikotun T, (2000). Storage structures and aflatoxin content of maize in five agroecological zones of Nigeria. Journal of Stored Products Research 36: 187–201.Search in Google Scholar

18. Bankole SA, Mabekoje OO, Enikuomehin OA. (2003). Fusarium spp. and fumonisin B1 in stored maize from Ogun State, Nigeria. Tropical Science 2003; 43: 76–79.Search in Google Scholar

19. Bankole SA, Mabekoje OO. (2004). Occurrence of aflatoxins and fumonisin pre-harvest maize from southwestern Nigeria. Food Additives and Contaminants 21: 251–255.Search in Google Scholar

20. Adejumo TO, Hettwer U, Karlovsky P. (2007). Occurrence of Fusarium species and trichothecenes in Nigerian maize International Journal of Food Microbiology, 116: 350–357Search in Google Scholar

21. Atehnkeng J, Ojiambo PS, Donner M, Ikotun T, Sikora RA, Cotty, PJ. (2008). Distribution and toxigenicity of Aspergillus species isolated from maize kernels from three agro-ecological zones in Nigeria, International Journal of Food Microbiology; 122: 74–84.Search in Google Scholar

22. Makun HA, Anjorin ST, Moronfoye B, Adejo FO, Afolabi,OA, Fagbayibo G, et al., (2010).Fungal and aflatoxin contamination of some human food commodities in Nigeria. African Journal of Food Science 4: 127-135.Search in Google Scholar

23. Merem EC, Twumasi Y, Wesley J, Isokpehi P, Shenge M, Fageir S. & Ochai S. (2017). Analyzing Rice Production Issues in the Niger State Area of Nigeria’s Middle Belt. Food and Public Health, 7(1), 7- 22. DOI: 10.5923/j.fph.20170701.0210.5923/j.fph.20170701.02Open DOISearch in Google Scholar

24. Kpodo K, Thrane U, Hald B. (2000). Fusaria and fumonisins in maize from Ghana and their co-occurrence with aflatoxins. International Journal of Food Microbiology, 61:147–157.Search in Google Scholar

25. Gonza´ slez HHL, Resnik SL, Pacin, AM. (2003). Mycoflora of freshly harvested flint corn from Northwestern Provinces in Argentina. Mycopathologia; 155: 207–211.Search in Google Scholar

26. Perrone G, Haidukowski M, Stea G, Epifani F, Bandyopadhyay R, Leslie JF, Logrieco A, (2014). Population structure and Aflatoxin production by Aspergillus Sect. Flavi from maize in Nigeria and Ghana. Food Microbiology, 41, 52-59. http://dx.doi.org/10.1016/j.fm.2013.12.00510.1016/j.fm.2013.12.005Open DOISearch in Google Scholar

27. Alborch, L, Bragulat, MR, Castellá, G, Abarca, ML, Cabañes, FJ (2012). Mycobiota and mycotoxin contamination of maize flours and popcorn kernels for human consumption commercialized in Spain. Food microbiology, 32(1), 97-103. doi:10.1016/j.fm.2012.04.01410.1016/j.fm.2012.04.014Open DOISearch in Google Scholar

28. Ehrlich KC, Cotty PJ, (2004). An isolate of A. flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene. Appl. Microbiol. Biotechnol. 65, 473-478.Search in Google Scholar

29. Donner M, Atehnkeng J, Sikora RA, Bandyopadhyay R, Cotty PJ, (2009). Distribution of Aspergillus section Flavi in soils of maize fields in three agroecological zones of Nigeria. Soil. Biol. Biochem. 41, 37-44.Search in Google Scholar

30. Piacentini, K.C.; Rocha, L.O.; Savi, G.D.; Carnielli-Queiroz, L.; De Carvalho Fontes, L.; Correa, B. (2019). Assessment of Toxigenic Fusarium Species and Their Mycotoxins in Brewing Barley Grains. Toxins , 11, 31. https://doi.org/10.3390/toxins1101003110.3390/toxins11010031Open DOISearch in Google Scholar

31. Xing F, Liu X, Wang L, Selvaraj JN, Jin N, Wang Y. & Liu Y. (2017). Distribution and variation of fungi and major mycotoxins in pre-and post-nature drying maize in North China Plain. Food control, 80, 244-251. http://dx.doi.org/10.1016/j.foodcont.2017.03.05510.1016/j.foodcont.2017.03.055Open DOISearch in Google Scholar

32. Streit E, Schatzmayr G, Tassis P, Tzika E, Marin D, Taranu I, et al. (2012). Current situation of mycotoxin contamination and co-occurrence in animal feed-focus on Europe. Toxins, 4(10), 788-809.Search in Google Scholar

33. Hassan ZU, Al Thani R, Balmas V, Migheli Q, Jaoua S (2019). Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control 104, 224–230. https://doi.org/10.1016/j.foodcont.2019.04.04510.1016/j.foodcont.2019.04.045Open DOISearch in Google Scholar

34. Hirst Sole LA, (1994). Algunos aspectos físicos y de procesamiento del maíz en grano y mazorca, con y sin tusa, almacenado en dos tipos de estructuras, con y sin tratamiento químico. Universidad del Valle de Guatemala. Ingeniería en Ciencias de Alimentos.Search in Google Scholar

35. Mendoza JR, Kok CR, Stratton J, Bianchini A, Hallen-Adams HE, (2017). Understanding the mycobiota of maize from the highlands of Guatemala, and implications for maize quality and safety. Crop Protection, 101, 5-11. http://dx.doi.org/10.1016/j.cropro.2017.07.00910.1016/j.cropro.2017.07.009Open DOISearch in Google Scholar

36. Marais GJ. (2000). Natural occurrence of mycotoxins in foods and feeds in South Africa. Submission to AFMA Technical Committee, Pp 88-92Search in Google Scholar

37. Peter J, Cotty PJ (1994). Comparison of four media for the isolation of Aspergillus flavus group fungi. Mycopathologia 125: 157-162.Search in Google Scholar

38. Klich MA (2002). Identification of common Aspergillus species. Utrecht: Central Bureau voor Schimmel cultures.Search in Google Scholar

39. Ehrlich KC, Kobbeman K, Montalbano BG, Cotty PJ. (2007). Aflatoxin producing Aspergillus species from Thailand. Int J Food Microbiol. 114:153–159.Search in Google Scholar

40. Pitt JI, Hocking AD. (2009). Fungi and food spoilage. London: Springer.Search in Google Scholar

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