<abstract xmlns="http://www.w3.org/1999/xhtml">The effect of dietary resveratrol (40 µg g−1 BW day−1) on cell-mediated immunity (the activity of spleen phagocytes and the proliferative response of lymphocytes) and liver histology (hepatocyte morphometry, lipid vacuoles, and glycogen granules) in adult medaka (<italic>Oryzias latipes</italic>) (aged two years, body weight BW = 0.49 g) were tested after ten days of feeding with experimental diets. The fish were fed either a commercial diet (control group C) or this same diet supplemented with resveratrol (group R) three times daily at 3% of body weight (BW). Fish fed feed with resveratrol had significantly higher intracellular phagocyte killing activity than did those in the control group (P &lt; 0.05). No differences in the sizes of hepatocytes or their nuclei were observed in the fish from groups C and R. The nucleo-cytoplasmic ratio ranged from 0.39 (± 0.03; group R) to 0.42 (± 0.03, group C) in adult medaka exposed to the two dietary treatments (P > 0.05). The results indicated that the diet supplemented with resveratrol at a dose of 40 µg g−1 BW day−1 had no impact on the liver tissues. Typical hepatocytes with lipids and glycogen were observed to the same extent in the vacuoles. Moreover, no mortality or pathological changes were noted in the fish studied. The diet containing resveratrol helped to maintain disease resistance without affecting the liver tissue after ten days of feeding.</abstract>

The effect of dietary resveratrol (40 µg g−1 BW day−1) on cell-mediated immunity (the activity of spleen phagocytes and the proliferative response of lymphocytes) and liver histology (hepatocyte morphometry, lipid vacuoles, and glycogen granules) in adult medaka (Oryzias latipes) (aged two years, body weight BW = 0.49 g) were tested after ten days of feeding with experimental diets. The fish were fed either a commercial diet (control group C) or this same diet supplemented with resveratrol (group R) three times daily at 3% of body weight (BW). Fish fed feed with resveratrol had significantly higher intracellular phagocyte killing activity than did those in the control group (P < 0.05). No differences in the sizes of hepatocytes or their nuclei were observed in the fish from groups C and R. The nucleo-cytoplasmic ratio ranged from 0.39 (± 0.03; group R) to 0.42 (± 0.03, group C) in adult medaka exposed to the two dietary treatments (P > 0.05). The results indicated that the diet supplemented with resveratrol at a dose of 40 µg g−1 BW day−1 had no impact on the liver tissues. Typical hepatocytes with lipids and glycogen were observed to the same extent in the vacuoles. Moreover, no mortality or pathological changes were noted in the fish studied. The diet containing resveratrol helped to maintain disease resistance without affecting the liver tissue after ten days of feeding.

Effect of dietary resveratrol on cell-mediated immunity and hepatocyte morphometry in the model organism medaka (<italic>Oryzias latipes</italic> Temminck &amp; Schlegel)

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

Fisheries & Aquatic Life

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of dietary resveratrol on cell-mediated immunity and hepatocyte morphometry in the model organism medaka ( Temminck & Schlegel)

The effect of dietary resveratrol (40 µg g−1 BW day−1) on cell-mediated immunity (the activity of spleen phagocytes and the proliferative response of lymphocytes) and liver histology (hepatocyte morphometry, lipid vacuoles, and glycogen granules) in adult medaka (Oryzias latipes) (aged two years, body weight BW = 0.49 g) were tested after ten days of feeding with experimental diets. The fish were fed either a commercial diet (control group C) or this same diet supplemented with resveratrol (group R) three times daily at 3% of body weight (BW). Fish fed feed with resveratrol had significantly higher intracellular phagocyte killing activity than did those in the control group (P  0.05). The results indicated that the diet supplemented with resveratrol at a dose of 40 µg g−1 BW day−1 had no impact on the liver tissues. Typical hepatocytes with lipids and glycogen were observed to the same extent in the vacuoles. Moreover, no mortality or pathological changes were noted in the fish studied. The diet containing resveratrol helped to maintain disease resistance without affecting the liver tissue after ten days of feeding.

The effect of dietary resveratrol (40 µg g−1 BW day−1) on cell-mediated immunity (the activity of spleen phagocytes and the proliferative response of...

Effect of dietary resveratrol on cell-mediated immunity and hepatocyte morphometry in the model organism medaka (Oryzias latipes Temminck & Schlegel)

Published Online: May 11, 2020

Page range: 33 - 38

Received: Dec 02, 2019

Accepted: Feb 17, 2020

DOI: https://doi.org/10.2478/aopf-2020-0004

Keywords
histology, immunity, medaka, Oryzias latipes, resveratrol

© 2020 Agata Kowalska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of dietary resveratrol on cell-mediated immunity and hepatocyte morphometry in the model organism medaka (Oryzias latipes Temminck & Schlegel)

Published Online: May 11, 2020

Page range: 33 - 38

Received: Dec 02, 2019

Accepted: Feb 17, 2020

DOI: https://doi.org/10.2478/aopf-2020-0004

Keywordshistology, immunity, medaka, Oryzias latipes, resveratrol

© 2020 Agata Kowalska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
histology, immunity, medaka, Oryzias latipes, resveratrol