1. bookVolume 27 (2019): Issue 4 (December 2019)
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Characterization, screening, and application of bacteria with probiotic properties isolated from the gut of Labeo calbasu (Hamilton)

Published Online: 27 Feb 2020
Page range: 178 - 189
Received: 27 Mar 2018
Accepted: 20 Nov 2019
Journal Details
License
Format
Journal
First Published
30 Dec 2008
Publication timeframe
4 times per year
Languages
English
Copyright
© 2020 Sciendo

Labeo calbasu is not reared in aquaculture because of the unavailability of proper feed and the risk of infection. The ability of probiotics to promote growth and immunological defense barriers in the gut has led to the use of autochthonous probiotic bacteria. Hence, the present study was conducted to screen potential probiotic bacteria from the gut of L. calbasu and to supplement the same to study the effect on growth and immunity. Strains with probiotic properties were assayed for exogenous enzyme production and identified based on phylogenetic analysis and nucleotide homology. Four of nine isolates showed promising antibacterial activity against Aeromonas hydrophila. The strains survived in relatively low pH and high bile concentrations and were investigated for hydrophobicity, autoaggregation, and coaggregation. Based on the positive results of this study, the LC1 and LC4 strains (analysed with 16S rRNA gene sequencing and showing similarity with Aneurinibacillus aneurinilyticus and Stenotrophomonas sp.) were further incorporated into diets to explore their probiotic effects on the growth performance and immunity of the fish. Administering the probiotics elevated growth parameters and benefitted the immune system by increasing the phagocytic index and decreasing the mortality rate with exposure to A. hydrophila.

Keywords

American Public Health Association 1998 – Standard methods for the examination of water and waste water – 20th edn. American Public Health Association, New York.Search in Google Scholar

Bairagi A., Sarkarghosh K., Sen S.K., Ray A.K. 2002 – Duck-weed (Lemna polyrrhiza) leaf meal as source of feedstuff in formulated diets for rohu (Labeo rohita) fingerlings after formulation with a fish intestinal bacterium – Bioresour. Technol. 85: 17-23.Search in Google Scholar

Balcazar J.L., de Blas I., Ruiz-Zarzuela I., Cunningham D., Vendrell D., Muzquiz J.L. 2006 – The role of probiotics in aquaculture – Vet. Microbiol. 114: 173-186.Search in Google Scholar

Balcazar J.L., Blas D., Zarzuela I.R., Vendrell D., Girones O., Muzquiz J.L. 2007a – Sequencing of variable regions of the 16S rRNA gene for identification of lactic acid bacteria isolated from the intestinal microbiota of healthy salmonids – Comp. Immunol. Microbiol. Infect. Dis. 30: 111-118.Search in Google Scholar

Balcazar J.L., de Blas I., Ruiz-Zarzuela I., Vendrell D., Girones O., Muzquiz J.L. 2007b – Enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (Oncorhynchus mykiss) – FEMS Immunol. Med. Mic. 51: 185-193.Search in Google Scholar

Bandyopadhyay P. 2004 – Improvement of growth, nutritional quality and immunity of Indian major carps through probiotic supplement – Ph.D. thesis, Vidyasagar University, Midnapore-721102, West Bengal. India.Search in Google Scholar

Barry T., Powell R., Gannon F.A. 1990 – A general method for the development of DNA probes for microorganisms – Nat. Biotechnol. 8: 233-236Search in Google Scholar

Bergey’s Manual of Systematics of Archaea and Bacteria 2015 – Bergey’s Manual Trust – John Wiley and Sons.Search in Google Scholar

Berwick V., Cheek L., Ball J. 2004 – Statistics review 12: Survival analysis – Crit. Care 8: 389-394.Search in Google Scholar

Bhatnagar A., Dhillon O. 2017 – Evaluation of optimum protein requirement and cost effective eco-friendly source for Labeo calbasu (Hamilton, 1822) – J. Fish. Aquat. Sci. 12: 273-283.Search in Google Scholar

Bhatnagar A., Lamba R. 2015 – Antimicrobial ability and growth promoting effects of feed supplemented with probiotic bacterium isolated from gut microflora of Cirrhinus mrigala – J. Integr. Agric. 14: 583-592.Search in Google Scholar

Bhatnagar A., Lamba R. 2017 – Molecular characterization and dosage application of autochthonous potential probiotic bacteria in Cirrhinus mrigala – J Fisheries Sci.com 11(2): 46-56.Search in Google Scholar

Bhatnagar A., Raparia S. 2014 – Optimum dietary inclusion level of Bacillus coagulans for growth and digestibility improvement for Catla catla (Hamilton) – Int. J. Curr. Res. Rev. 6(7): 1–10.Search in Google Scholar

Bhatnagar A., Raparia S., Kumari S. 2012 – Isolation and Influence of Bacillus coagulans CC1 on growth performance and digestive enzyme activities of Catla catla –J. Nat. Sci. Sustainable Technol. 6: 237-253Search in Google Scholar

Carnevali O., de Vivo L., Sulpizio R., Gioacchini G., Olivotto I., Silvi S., Cresci A. 2006 – Growth improvement by probiotic in European sea brass juveniles (Dicentrachus labrax, L.), with particular attention to IGF-1, myostatin and cortiosol gene expression – Aquaculture 258: 430-438Search in Google Scholar

Conway P.L., Gorbach S.L., Goldin B.R. 1987 – Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells – J. Dairy Sci. 70: 1-12.Search in Google Scholar

Das D., Chatterjee S.N., Dangar T.K. 2016 – Characterization and mosquitocidal potential of the soil bacteria Aneurinibacillus aneurinilyticus isolated from Burdwan, West Bengal, India – Proc. Natl. Acad. Sci. India Sect. B Biol. Sci. 86: 707-713.Search in Google Scholar

Del Re B., Sgorbati D., Miglioli M., Palenzona D. 2000 – Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum – Lett. Appl. Microbiol. 31: 438-442.Search in Google Scholar

Duncan D.B. 1955 – Multiple range and Multiple F Tests – Biometrics 11: 1-42.Search in Google Scholar

Ellis A.E. 1988 – Fish Vaccination – Academic Press, London, 84 p.Search in Google Scholar

Garg S.K., Bhatnagar A., Kalla A., Johal M.S. 2002 – Experimental Ichthyology – CBS Publications and Distributors, New Delhi.Search in Google Scholar

Ghosh K., Sen S.K., Ray A.K. 2002 – Characterization of Bacilli isolated from gut of rohu, Labeo rohita, fingerlings and its significance in digestion – J. Appl. Aquacult. 12: 33-42.Search in Google Scholar

Ghosh S., Sinha A., Sahu C. 2007 – Isolation of putative probionts from the intestines of Indian major carps – Isr. J. Aquacult. – Bamidgeh 59: 127-132.Search in Google Scholar

Gilliland S.E., Staley T.E., Bush L.J. 1984 – Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct – J. Dairy Sci. 67: 3045-3051.Search in Google Scholar

Giri S.S., Sukumaran V., Oviya M. 2013 – Potential probiotic Lactobacillus plantarum VSG-3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita – Fish Shellfish Immunol. 34: 660-666.Search in Google Scholar

Handley P.S., Harty D.W.S., Wyatt J.E., Brown C.R., Doran J.P., Gibbs A.C.C. 1987 – A comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius – J Gen. Microbiol. 133: 3207-3217.Search in Google Scholar

Holt G.J., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. 1994 – Bergey’s manual of determinative bacteriology – 9th edn. Philadelphia, PA: Lippincott Williams and Wilkins.Search in Google Scholar

Hossain M.M., Uddin M.N., Islam M.N., Chakraborty S.C., Kamal M. 1999 – Study on the intestinal bacteria of Labeo rohita (Ham.) – Bangladesh J. Fish. Res. 3: 63-66.Search in Google Scholar

Kos B., Šuškoviæ J., Vukoviæ S., Šimpraga M., Frece J., Matošiæ S. 2003 – Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92 – J. Appl. Microbiol. 94: 981-987Search in Google Scholar

Lyon W.J., Glatz B.A. 1993 – Isolation and purification of propionicin PLG-1, a bacteriocin produced by a strain of Propiobacterium theonii – Appl. Environ. Microbiol. 59: 83-87.Search in Google Scholar

Mohapatra S., Chakraborty T., Prusty A.K., Das P., Paniprasad K., Mohanta K.N. 2012 – Use of different microbial probiotics in the diet of rohu, Labeo rohita fingerlings: effects on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal microflora – Aquac. Nutr. 18: 1-11.Search in Google Scholar

Nandi A., Dan S.K., Banerjee G., Ghosh P., Ghosh K., Ringo O., Ray A.K. 2017 – Probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts – Probiotics Antimicro Prot. 9: 12-21.Search in Google Scholar

Nayak S.K. 2010 – Role of gastrointestinal microbiota in fish – Aquacult. Res. 41: 1553-1726.Search in Google Scholar

Nayak S.K., Mukherjee S.C. 2011 – Screening of gastrointestinal bacteria of Indian major carps – Aquacult. Res. 42: 1034-1041.Search in Google Scholar

O’Sullivan D.J. 2001 – Screening of intestinal microflora for effective probiotic bacteria – J. Agric. Food Chem. 49: 1751-1760.Search in Google Scholar

Park K.H., Jeong H.D. 1996 – Enhanced resistance against Edwardsiella tarda infection in tilapia (Oreochromis niloticus) by administration of protein-bound polysaccharide – Aquaculture 141: 135-143.Search in Google Scholar

Pennacchia C., Ercolini D., Blaiotta G., Pepe O., Mauriello G., Villani F. 2004 – Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics – Meat Sci. 67: 309-317.Search in Google Scholar

Rani S., Garg S.K., Sabhlok V.P., Bhatnagar A. 2004 – Intestinal enzyme activity and enzyme producing microbial flora in relation to feeding behavior in some brackish water teleosts – J. Aquacult. 12: 55-68.Search in Google Scholar

Ray A.K., Ghosh K., Ringo E. 2012 – Enzyme-producing bacteria isolated from fish gut – A review – Aquac. Nutr. 18: 465-492.Search in Google Scholar

Rojas M., Conway P.L. 1996 – Colonization by lactobacilli of piglet small intestinal mucus – J. Appl. Bacteriol. 81: 474-480.Search in Google Scholar

Sahoo P.K., Mukherjee S.C., Sahoo S.K. 1998 – Aeromonas hydrophila versus Edwardsiella tarda: a pathoanatomical study in Clarias batrachus – J. Aquacult. 6: 57-66.Search in Google Scholar

Salvadori L., Gioia D.D., Fava F., Barberio C. 2006 – Degradation of low-ethoxylated nonylphenols by a Stenotrophomonas strain and development of new phylogenetic probes for Stenotrophomonas spp detection – Curr. Microbiol. 52: 13-20.Search in Google Scholar

Sansawat A., Thirabunyanon M. 2009 – Anti-Aeromonas hydrophila activity and characterisation of novel probiotic strains of Bacillus subtilis isolated from the gastrointestinal tract of giant freshwater prawns – Maejo. Int. J. Sci. Technol. 3: 77-87.Search in Google Scholar

Savage D.C. 1992 – Growth phase cellular hydrophobicity and adhesion in vitro of Lactobacilli colonizing the keratinizing gastric epithelium in the mouse – Appl. Environ. Microbiol. 58: 1992-1995.Search in Google Scholar

Siwicki A.K., Anderson D.P., Rumsey G.L. 1994 – Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furuncuclosis – Vet. Immunol. Immunopathol. 41: 125-139.Search in Google Scholar

Vine N.G., Leukes W.D., Kaiser H., Daya S., Baxter J., Hecht T. 2004 – Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus – J. Fish. Dis. 27: 319-326.Search in Google Scholar

Zhu H., Qu F., Zhu L.H. 1993 – Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride – Nucleic Acids Res. 21: 5279-5280.Search in Google Scholar

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