<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates was investigated <italic>in vitro</italic> on cell cultures and <italic>in vivo</italic> on rainbow trout, <italic>Oncorhynchus mykiss</italic> (Walbaum), fingerlings. Experimental indications were that the Ukrainian IPNV isolates have affinity with reference European strains. During the reproduction of these isolates in cell cultures of FHM (fat head minnow), RTG-2 (rainbow trout gonads), and BF-2 (bluegill caudal peduncle), complicated degenerative changes were visible that finally led to the full destruction of cell monolayers. The experimental infection of rainbow trout fingerlings resulted in typical disease symptoms that were systemic. However, obvious evidence of viral infection was noted in single individuals only, and the majority of experimental fish died without visible disease symptoms. During the study of physicochemical properties, it was noted that Ukrainian isolates completely lost their infectivity with chloroform treatment and heating to 60°C. This proved that IPNV isolates are resistant to Ion concentrations in the range of pH 3.0 to 12.0.</p></abstract>

The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates was investigated in vitro on cell cultures and in vivo on rainbow trout, Oncorhynchus mykiss (Walbaum), fingerlings. Experimental indications were that the Ukrainian IPNV isolates have affinity with reference European strains. During the reproduction of these isolates in cell cultures of FHM (fat head minnow), RTG-2 (rainbow trout gonads), and BF-2 (bluegill caudal peduncle), complicated degenerative changes were visible that finally led to the full destruction of cell monolayers. The experimental infection of rainbow trout fingerlings resulted in typical disease symptoms that were systemic. However, obvious evidence of viral infection was noted in single individuals only, and the majority of experimental fish died without visible disease symptoms. During the study of physicochemical properties, it was noted that Ukrainian isolates completely lost their infectivity with chloroform treatment and heating to 60°C. This proved that IPNV isolates are resistant to Ion concentrations in the range of pH 3.0 to 12.0.

Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout <italic>(Oncorhynchus Mykiss)</italic> Fingerlings

Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, 135 Obukhivska Street, 03164 Kyiv, Ukraine

Fisheries & Aquatic Life

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout  Fingerlings

The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates...

Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout (Oncorhynchus Mykiss) Fingerlings

Article Category: Research Paper

Published Online: Jul 15, 2014

Page range: 127 - 133

Received: Jun 25, 2013

Accepted: Feb 27, 2014

DOI: https://doi.org/10.2478/aopf-2014-0012

Keywords
cell culture, cytopathogenic effect, Infectious Pancreatic Necrosis Virus (IPNV), rainbow trout

© 2014 Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout (Oncorhynchus Mykiss) Fingerlings

Article Category: Research Paper

Published Online: Jul 15, 2014

Page range: 127 - 133

Received: Jun 25, 2013

Accepted: Feb 27, 2014

DOI: https://doi.org/10.2478/aopf-2014-0012

Keywordscell culture, cytopathogenic effect, Infectious Pancreatic Necrosis Virus (IPNV), rainbow trout

© 2014 Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cell culture, cytopathogenic effect, Infectious Pancreatic Necrosis Virus (IPNV), rainbow trout