<abstract xmlns="http://www.w3.org/1999/xhtml"> Oxidative stress, biochemical and enzymological biomarkers were compared among rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.) to determine the mechanisms of hepatotoxicity caused by chloramine-T bath treatment. The fish were exposed to chloramine-T at a concentration of 9 g m-3 for 20 min three times daily every three days. The control groups of fish, which were not exposed, were handled in the same way as the chloramine-T treatment groups. Chloramine-T treatment varies among fish species; for example, such treatment baths markedly decrease the carbonyl derivate levels of oxidative protein and aminotransferase activities, the elevation of which is a compensatory mechanism for impaired metabolism, but only in rainbow trout livers. No significant changes were noted in oxidative stress biomarkers between the control and chloramine-treated brown trout groups. Grayling chloramine-T exposure resulted in significant elevation in severe oxidative stress biomarker levels. Increased carbonyl derivates of oxidative protein can modify aminotransferase and lactate dehydrogenase activity and lactate and pyruvate levels, all of which cause primarily increased enzyme activity because of oxidative stress in the livers of the chloramine-exposed fish. The present study indicates that chloramine-T at a dose of 9 g m-3 can partially attenuate oxidative stress, and can be used for the prophylactic treatment of rainbow and brown trout.</abstract>

Oxidative stress, biochemical and enzymological biomarkers were compared among rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.) to determine the mechanisms of hepatotoxicity caused by chloramine-T bath treatment. The fish were exposed to chloramine-T at a concentration of 9 g m-3 for 20 min three times daily every three days. The control groups of fish, which were not exposed, were handled in the same way as the chloramine-T treatment groups. Chloramine-T treatment varies among fish species; for example, such treatment baths markedly decrease the carbonyl derivate levels of oxidative protein and aminotransferase activities, the elevation of which is a compensatory mechanism for impaired metabolism, but only in rainbow trout livers. No significant changes were noted in oxidative stress biomarkers between the control and chloramine-treated brown trout groups. Grayling chloramine-T exposure resulted in significant elevation in severe oxidative stress biomarker levels. Increased carbonyl derivates of oxidative protein can modify aminotransferase and lactate dehydrogenase activity and lactate and pyruvate levels, all of which cause primarily increased enzyme activity because of oxidative stress in the livers of the chloramine-exposed fish. The present study indicates that chloramine-T at a dose of 9 g m-3 can partially attenuate oxidative stress, and can be used for the prophylactic treatment of rainbow and brown trout.

Effects of chloramine-T exposure on oxidative stress biomarkers and liver biochemistry of rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.)

Department of Zoology, Institute of Biology and Environmental Protection, Pomeranian University, Słupsk, Poland

Department of Animal Physiology, Institute of Biology and Environmental Protection, Pomeranian University, Słupsk, Poland

Department of Salmonid Research in Rutki Inland Fisheries Institute in Olsztyn, Poland

Fisheries & Aquatic Life

Oxidative stress, biochemical and enzymological biomarkers were compared among rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.),...

Effects of chloramine-T exposure on oxidative stress biomarkers and liver biochemistry of rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.)

Published Online: May 11, 2013

Page range: 41 - 51

DOI: https://doi.org/10.2478/aopf-2013-0005

Keywords
chloramine-T, oxidative stress, aminotransferases, lactate dehydrogenase, liver

This content is open access.

Effects of chloramine-T exposure on oxidative stress biomarkers and liver biochemistry of rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.)

Published Online: May 11, 2013

Page range: 41 - 51

DOI: https://doi.org/10.2478/aopf-2013-0005

Keywordschloramine-T, oxidative stress, aminotransferases, lactate dehydrogenase, liver

This content is open access.

Keywords
chloramine-T, oxidative stress, aminotransferases, lactate dehydrogenase, liver