<abstract xmlns="http://www.w3.org/1999/xhtml">

This study evaluated the individual and combined effects of <italic>L. plantarum</italic> and <italic>L. reuteri</italic> on the growth performance, digestive enzymes, antioxidant, and immunity-related genes in zebrafish. Six hundred zebrafish (38.19±1.4 mg) were randomized into twelve tanks (50 fish per), and fed prepared diets comprising un-supplemented (T0), 1 ×108 CFU/g <italic>L. plantarum</italic> (T1), 1 ×1011 CFU/kg <italic>L. reuteri</italic> (T2), and 1 ×108 CFU/g <italic>L. plantarum</italic> + 1 ×1011 CFU/kg <italic>L. reuteri</italic> (T3) for 8 weeks. Experimental diets had meaningful effects on weight gain (WG), final weight (FW), and the highest WG and FW were seen in T3. Fish-fed supplemented diets had higher amylase and lipase activities. No significant differences were seen in protease, pepsin, chymotrypsin, and trypsin activities between treatments. The supplemented groups showed a significant increase in lysozyme activity and total immunoglobulin levels. Meaningful differences were noticed in ACH50, lysozyme activity, total immunoglobulin, and IgM levels. ALT levels were markedly higher in T2 and T3. Catalase (CAT), total antioxidant, and glutathione peroxidase (GPX) activity were significantly impacted by the experimental diets. The experimental diets showed no marked impact on superoxide dismutase (SOD) activity, and malondialdehyde (MDA) values. Relative expressions of <italic>CAT</italic>, <italic>GPX</italic>, and <italic>SOD</italic> genes were notably upper in T3. Moreover, <italic>IL1, LYZ,</italic> and <italic>TNFα</italic> gene expressions were significantly enhanced in T3 diets. The challenge test <italic>with A. hydrophila</italic> showed that zebrafish-fed L. <italic>plantarum</italic> and <italic>L. reuteri</italic> had a lower mortality rate than the control. Consequently, the combination of <italic>L. reuteri</italic> and <italic>L. plantarum</italic> is proposed to improve growth efficiency, immunity, and reduce the negative effects of <italic>A. hydrophila</italic> infection in zebrafish.
</abstract>

This study evaluated the individual and combined effects of L. plantarum and L. reuteri on the growth performance, digestive enzymes, antioxidant, and immunity-related genes in zebrafish. Six hundred zebrafish (38.19±1.4 mg) were randomized into twelve tanks (50 fish per), and fed prepared diets comprising un-supplemented (T0), 1 ×108 CFU/g L. plantarum (T1), 1 ×1011 CFU/kg L. reuteri (T2), and 1 ×108 CFU/g L. plantarum + 1 ×1011 CFU/kg L. reuteri (T3) for 8 weeks. Experimental diets had meaningful effects on weight gain (WG), final weight (FW), and the highest WG and FW were seen in T3. Fish-fed supplemented diets had higher amylase and lipase activities. No significant differences were seen in protease, pepsin, chymotrypsin, and trypsin activities between treatments. The supplemented groups showed a significant increase in lysozyme activity and total immunoglobulin levels. Meaningful differences were noticed in ACH50, lysozyme activity, total immunoglobulin, and IgM levels. ALT levels were markedly higher in T2 and T3. Catalase (CAT), total antioxidant, and glutathione peroxidase (GPX) activity were significantly impacted by the experimental diets. The experimental diets showed no marked impact on superoxide dismutase (SOD) activity, and malondialdehyde (MDA) values. Relative expressions of CAT, GPX, and SOD genes were notably upper in T3. Moreover, IL1, LYZ, and TNFα gene expressions were significantly enhanced in T3 diets. The challenge test with A. hydrophila showed that zebrafish-fed L. plantarum and L. reuteri had a lower mortality rate than the control. Consequently, the combination of L. reuteri and L. plantarum is proposed to improve growth efficiency, immunity, and reduce the negative effects of A. hydrophila infection in zebrafish.

Dietary administration of <italic>Lactobacillus reuteri</italic> and <italic>Lactobacillus plantarum</italic> reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish (<italic>Danio rerio</italic>) against <italic>Aeromonas hydrophila</italic> infection

Faculty of Biological Science, Kharazmi University

Faculty of Natural Resources, University of Zabol

Faculty of Veterinary Medicine, Amol University of Special Modern Technologies

Faculty of Agriculture and Natural Resources, Gonbad Kavous University

Çanakkale Onsekiz Mart University, Department of Aquaculture

Department of Biology, collage of science

University of science and culture, ACECR, Tehran branch

Unit of Bee Research and Honey Production

Research Center for Advanced Materials Science (RCAMS), King Khalid University

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Dietary administration of  and  reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish () against  infection

This study evaluated the individual and combined effects of L. plantarum and L. reuteri on the growth performance, digestive enzymes, antioxidant, and...

Dietary administration of Lactobacillus reuteri and Lactobacillus plantarum reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila infection

Published Online: Jul 19, 2024

Page range: -

Received: Feb 22, 2024

Accepted: Jun 03, 2024

DOI: https://doi.org/10.2478/aoas-2024-0066

Keywords
aquaculture, probiotics

© 2024 Delaram Eslimi Esfahani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Dietary administration of Lactobacillus reuteri and Lactobacillus plantarum reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila infection

Published Online: Jul 19, 2024

Page range: -

Received: Feb 22, 2024

Accepted: Jun 03, 2024

DOI: https://doi.org/10.2478/aoas-2024-0066

Keywordsaquaculture, probiotics

© 2024 Delaram Eslimi Esfahani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
aquaculture, probiotics