<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to evaluate the effects of <italic>Mentha piperita</italic> methanolic extract (MPE) on <italic>Cyprinus carpio</italic> intestinal microbiota, including total microorganisms gram-negative bacteria, lactic acid bacteria, and fungi count. Liver and intestinal histopathology, and the activity of liver enzymes, were also used to evaluate the possible side effects of MPE. A total of 96 healthy <italic>C. carpio</italic> fries (76.76±20.26 g) were allocated to four treatment groups with three replications in a completely randomized design. The fries were fed with diets containing 0, 0.5, 1, and 2% extract for 56 days at the rate of 2% of body weight during the experiment. Results showed a significant decrease in total microorganisms, enteric gram-negative bacteria, and total fungi counts (p&lt;0.05). The total lactic acid bacteria count in 0.5% treatment was significantly lower than in control and 2% treated fish (P&lt;0.05). MPE did not affect AST, leading to a significant increase in ALT levels. Simultaneously, ALP represented significantly higher activity in the control group (P>0.05). Microscopic findings revealed marked lesions, including congestion and cell degeneration in the livers of the three groups of fish fed with the extract. The intestinal folds were shortened and blunted in the treatment groups. Furthermore, the intestinal mucosa was necrotic, and the lamina propria was significantly thickened with mononuclear inflammatory cells (P&lt;0.05). Although MPE significantly affects intestinal microbiota, its consumption at 2% is not recommended for <italic>C. carpio</italic> due to the lesions made in the liver and intestine.</p>
</abstract>

This study aimed to evaluate the effects of Mentha piperita methanolic extract (MPE) on Cyprinus carpio intestinal microbiota, including total microorganisms gram-negative bacteria, lactic acid bacteria, and fungi count. Liver and intestinal histopathology, and the activity of liver enzymes, were also used to evaluate the possible side effects of MPE. A total of 96 healthy C. carpio fries (76.76±20.26 g) were allocated to four treatment groups with three replications in a completely randomized design. The fries were fed with diets containing 0, 0.5, 1, and 2% extract for 56 days at the rate of 2% of body weight during the experiment. Results showed a significant decrease in total microorganisms, enteric gram-negative bacteria, and total fungi counts (p<0.05). The total lactic acid bacteria count in 0.5% treatment was significantly lower than in control and 2% treated fish (P<0.05). MPE did not affect AST, leading to a significant increase in ALT levels. Simultaneously, ALP represented significantly higher activity in the control group (P>0.05). Microscopic findings revealed marked lesions, including congestion and cell degeneration in the livers of the three groups of fish fed with the extract. The intestinal folds were shortened and blunted in the treatment groups. Furthermore, the intestinal mucosa was necrotic, and the lamina propria was significantly thickened with mononuclear inflammatory cells (P<0.05). Although MPE significantly affects intestinal microbiota, its consumption at 2% is not recommended for C. carpio due to the lesions made in the liver and intestine.

Effects of Dietary Supplementation of Peppermint Extract on Growth Performance, Intestinal Microbiota, Liver and Intestine Histopathology of <italic>Cyprinus carpio</italic>

Department of Fisheries, Faculty of Natural Resources and Environment

Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine

Department of Food Science and Technology, Faculty of Agriculture

Department of Pathobiology, Faculty of Veterinary Medicine

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of Dietary Supplementation of Peppermint Extract on Growth Performance, Intestinal Microbiota, Liver and Intestine Histopathology of 

This study aimed to evaluate the effects of Mentha piperita methanolic extract (MPE) on Cyprinus carpio intestinal microbiota, including total microorganisms gram-negative bacteria, lactic acid bacteria, and fungi count. Liver and intestinal histopathology, and the activity of liver enzymes, were also used to evaluate the possible side effects of MPE. A total of 96 healthy C. carpio fries (76.76±20.26 g) were allocated to four treatment groups with three replications in a completely randomized design. The fries were fed with diets containing 0, 0.5, 1, and 2% extract for 56 days at the rate of 2% of body weight during the experiment. Results showed a significant decrease in total microorganisms, enteric gram-negative bacteria, and total fungi counts (p0.05). Microscopic findings revealed marked lesions, including congestion and cell degeneration in the livers of the three groups of fish fed with the extract. The intestinal folds were shortened and blunted in the treatment groups. Furthermore, the intestinal mucosa was necrotic, and the lamina propria was significantly thickened with mononuclear inflammatory cells (P<0.05). Although MPE significantly affects intestinal microbiota, its consumption at 2% is not recommended for C. carpio due to the lesions made in the liver and intestine.

This study aimed to evaluate the effects of Mentha piperita methanolic extract (MPE) on Cyprinus carpio intestinal microbiota, including total microorganisms...

Effects of Dietary Supplementation of Peppermint Extract on Growth Performance, Intestinal Microbiota, Liver and Intestine Histopathology of Cyprinus carpio

Published Online: Nov 13, 2023

Page range: 1191 - 1199

Received: Nov 30, 2022

Accepted: Apr 05, 2023

DOI: https://doi.org/10.2478/aoas-2023-0042

Keywords
carp, mentha, intestinal microbiota, liver,

© 2023 Ali Baghalian et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of Dietary Supplementation of Peppermint Extract on Growth Performance, Intestinal Microbiota, Liver and Intestine Histopathology of Cyprinus carpio

Published Online: Nov 13, 2023

Page range: 1191 - 1199

Received: Nov 30, 2022

Accepted: Apr 05, 2023

DOI: https://doi.org/10.2478/aoas-2023-0042

Keywordscarp, mentha, intestinal microbiota, liver,

© 2023 Ali Baghalian et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
carp, mentha, intestinal microbiota, liver,