<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>DNA barcoding and mini-barcoding involve Cytochrome Oxidase Subunit I (<italic>COI</italic>) gene in mitochondrial genome and are used for accurate identification of species and biodiversity. The basic goal of the current study is to develop a complete reference database of fishes. It also evaluates the applicability of <italic>COI</italic> gene to identify fish at the species level with other aspects i.e., as Kimura 2 parameter (K2P) distance. The mean observed length of the sequence was ranging from 500 to 700 base pairs for fish species in DNA barcoding and from 80 to 650 base pairs for DNA mini-barcoding. This method describes the status of known to unknown samples but it also facilitates the detection of previously un-sampled species at distinct level. So, mini-barcoding is a method which focuses on the analysis of short-length DNA markers and has been demonstrated to be effective for species identification of processed food containing degraded DNA. While DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample. They may contain entire organisms or a single environmental sample containing degraded DNA. Despite DNA barcoding, mini-barcoding and metabarcoding are efficient methods for species identification which are helpful in conservation and proper management of biodiversity. It aids researchers to take an account of genetic as well as evolutionary relationships by collecting their morphological, distributional and molecular data. Overall, this paper discusses DNA barcoding technology and how it has been used to various fish species, as well as its universality, adaptability, and novel approach to DNA-based species identification.</p>
</abstract>

DNA barcoding and mini-barcoding involve Cytochrome Oxidase Subunit I (COI) gene in mitochondrial genome and are used for accurate identification of species and biodiversity. The basic goal of the current study is to develop a complete reference database of fishes. It also evaluates the applicability of COI gene to identify fish at the species level with other aspects i.e., as Kimura 2 parameter (K2P) distance. The mean observed length of the sequence was ranging from 500 to 700 base pairs for fish species in DNA barcoding and from 80 to 650 base pairs for DNA mini-barcoding. This method describes the status of known to unknown samples but it also facilitates the detection of previously un-sampled species at distinct level. So, mini-barcoding is a method which focuses on the analysis of short-length DNA markers and has been demonstrated to be effective for species identification of processed food containing degraded DNA. While DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample. They may contain entire organisms or a single environmental sample containing degraded DNA. Despite DNA barcoding, mini-barcoding and metabarcoding are efficient methods for species identification which are helpful in conservation and proper management of biodiversity. It aids researchers to take an account of genetic as well as evolutionary relationships by collecting their morphological, distributional and molecular data. Overall, this paper discusses DNA barcoding technology and how it has been used to various fish species, as well as its universality, adaptability, and novel approach to DNA-based species identification.

Pragmatic applications of DNA barcoding markers in identification of fish species – A review

Government Sadiq College Women University

Department of Entomology, Faculty of Agriculture and Environmental

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

DNA barcoding and mini-barcoding involve Cytochrome Oxidase Subunit I (COI) gene in mitochondrial genome and are used for accurate identification of species...

Pragmatic applications of DNA barcoding markers in identification of fish species – A review

Published Online: May 03, 2023

Page range: 363 - 389

Received: Mar 11, 2022

Accepted: Sep 27, 2022

DOI: https://doi.org/10.2478/aoas-2022-0073

Keywords
DNA barcoding, DNA mini-barcoding, metabarcoding, cytochrome c oxidase I, authentication

© 2023 Saima Naz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Pragmatic applications of DNA barcoding markers in identification of fish species – A review

Published Online: May 03, 2023

Page range: 363 - 389

Received: Mar 11, 2022

Accepted: Sep 27, 2022

DOI: https://doi.org/10.2478/aoas-2022-0073

KeywordsDNA barcoding, DNA mini-barcoding, metabarcoding, cytochrome c oxidase I, authentication

© 2023 Saima Naz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
DNA barcoding, DNA mini-barcoding, metabarcoding, cytochrome c oxidase I, authentication