<abstract xmlns="http://www.w3.org/1999/xhtml">

The effects of a functional additive (Sanacore® GM; SAN) on immune and antioxidant indices, and the resistance of gilthead seabream (<italic>Sparus aurata</italic>) against <italic>Vibrio alginolyticus</italic> infection were investigated. For this, four diets containing 0% (the control), 0.1%, 0.2%, and 0.4% SAN were offered to triplicated groups of fish (20–23 g) for ten weeks. Subsequently, fish were injected intraperitoneally with <italic>V. alginolyticus</italic> and monitored for further ten days. Feeding the fish on SAN-supplemented diets showed positive effects on leukocyte counts and its differential percentages. Serum lysozyme activity and total immunoglobulin values, as well as blood phagocytic activity and indices, were linearly and quadratically higher in SAN-fed fish, especially at the 0.4% SAN diet. Similarly, linear and quadratic increases in catalase, superoxide dismutase, and total antioxidant capacity were observed in SAN-fed fish, particularly at the 0.4% SAN diet. Conversely, malondialdehyde values decreased in SAN-fed fish compared with the control group, which showed its highest value. The highest expression of the <italic>IL-1β</italic> gene coupled with the lowest <italic>TNF-α</italic> and <italic>HSP70</italic> genes expressions was found in the fish fed with the 0.4% SAN. On the other hand, fish fed on the control diet showed the lowest <italic>IL-1β</italic> gene coupled with the highest <italic>TNF-α</italic> and <italic>HSP70</italic> genes expressions. After bacterial infection, most of the control fish died with a relative percent of survival of 5.0%; meanwhile feeding gilthead seabream on SAN-enriched diets significantly enhanced their protection against <italic>V. alginolyticus</italic> infection fed on the 0.4% SAN diet showed 100% survival. The SAN administration to gilthead seabream especially at the 0.4% level led to significant promotions in antioxidative and immune responses and augmented the fish resistance to <italic>V. alginolyticus</italic> infection.
</abstract>

The effects of a functional additive (Sanacore® GM; SAN) on immune and antioxidant indices, and the resistance of gilthead seabream (Sparus aurata) against Vibrio alginolyticus infection were investigated. For this, four diets containing 0% (the control), 0.1%, 0.2%, and 0.4% SAN were offered to triplicated groups of fish (20–23 g) for ten weeks. Subsequently, fish were injected intraperitoneally with V. alginolyticus and monitored for further ten days. Feeding the fish on SAN-supplemented diets showed positive effects on leukocyte counts and its differential percentages. Serum lysozyme activity and total immunoglobulin values, as well as blood phagocytic activity and indices, were linearly and quadratically higher in SAN-fed fish, especially at the 0.4% SAN diet. Similarly, linear and quadratic increases in catalase, superoxide dismutase, and total antioxidant capacity were observed in SAN-fed fish, particularly at the 0.4% SAN diet. Conversely, malondialdehyde values decreased in SAN-fed fish compared with the control group, which showed its highest value. The highest expression of the IL-1β gene coupled with the lowest TNF-α and HSP70 genes expressions was found in the fish fed with the 0.4% SAN. On the other hand, fish fed on the control diet showed the lowest IL-1β gene coupled with the highest TNF-α and HSP70 genes expressions. After bacterial infection, most of the control fish died with a relative percent of survival of 5.0%; meanwhile feeding gilthead seabream on SAN-enriched diets significantly enhanced their protection against V. alginolyticus infection fed on the 0.4% SAN diet showed 100% survival. The SAN administration to gilthead seabream especially at the 0.4% level led to significant promotions in antioxidative and immune responses and augmented the fish resistance to V. alginolyticus infection.

Effects of a Commercial Feed Additive (Sanacore® GM) on Immune-Antioxidant Profile and Resistance of Gilthead Seabream (<italic>Sparus aurata</italic>) Against <italic>Vibrio alginolyticus</italic> Infection

Central Laboratory for Aquaculture Research, Agriculture Research Center

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine

Department of Fish Health and Disease, Faculty of Fish Resources

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of a Commercial Feed Additive (Sanacore GM) on Immune-Antioxidant Profile and Resistance of Gilthead Seabream () Against  Infection

The effects of a functional additive (Sanacore® GM; SAN) on immune and antioxidant indices, and the resistance of gilthead seabream (Sparus aurata) against...

Effects of a Commercial Feed Additive (Sanacore^® GM) on Immune-Antioxidant Profile and Resistance of Gilthead Seabream (Sparus aurata) Against Vibrio alginolyticus Infection

Published Online: Jan 27, 2023

Page range: 185 - 193

Received: Apr 01, 2022

Accepted: Jun 24, 2022

DOI: https://doi.org/10.2478/aoas-2022-0053

Keywords
feed supplement, gilthead seabream, antioxidative status, immune response, infection

© 2022 Mohsen Abdel-Tawwab et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of a Commercial Feed Additive (Sanacore® GM) on Immune-Antioxidant Profile and Resistance of Gilthead Seabream (Sparus aurata) Against Vibrio alginolyticus Infection

Published Online: Jan 27, 2023

Page range: 185 - 193

Received: Apr 01, 2022

Accepted: Jun 24, 2022

DOI: https://doi.org/10.2478/aoas-2022-0053

Keywordsfeed supplement, gilthead seabream, antioxidative status, immune response, infection

© 2022 Mohsen Abdel-Tawwab et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of a Commercial Feed Additive (Sanacore^® GM) on Immune-Antioxidant Profile and Resistance of Gilthead Seabream (Sparus aurata) Against Vibrio alginolyticus Infection

Keywords
feed supplement, gilthead seabream, antioxidative status, immune response, infection