<abstract xmlns="http://www.w3.org/1999/xhtml">This study aimed to determine the effect of dietary oregano (<italic>Origanum vulgare</italic>) essential oil (EO) on the growth performance, digestive enzyme activity, intestinal histomorphology, and intestinal microbiota of the Black Sea salmon juvenile (<italic>Salmo labrax)</italic>. Fish were fed diets different levels of oregano EO such as 50, 100, 200, and 400 mg kg−1. For this purpose, a total of 675 fish were distributed randomly in triplicate into 5 experiment groups in 15 experiment tanks. Fish with average initial weights of 3.52±0.01 g were fed by hand at 3% of live weight for 90 days. At the end of the study, feeding with oregano EO supplementation did not significantly affect the growth performance of fish. Except for pepsin, there were no significant differences in the activity of digestive enzymes among the control and oregano EO groups. Besides, oregano EO at the doses of 50 or 400 mg kg−1 may have the potential to increase the surface area required for digestion by increasing intestinal villi length. Moreover, all doses of oregano EO showed antimicrobial properties by decreasing the count of lactic acid bacteria in the intestine. Supplementation with 100 and 200 mg kg−1 oregano EO in diets decreased the total coliform, <italic>Escherichia coli</italic> and lactic acid bacteria counts when compared to the control group. The results showed that oregano EO may positively affect digestion and absorption without adverse effects on the growth performance of Black Sea salmon juvenile.</abstract>

This study aimed to determine the effect of dietary oregano (Origanum vulgare) essential oil (EO) on the growth performance, digestive enzyme activity, intestinal histomorphology, and intestinal microbiota of the Black Sea salmon juvenile (Salmo labrax). Fish were fed diets different levels of oregano EO such as 50, 100, 200, and 400 mg kg−1. For this purpose, a total of 675 fish were distributed randomly in triplicate into 5 experiment groups in 15 experiment tanks. Fish with average initial weights of 3.52±0.01 g were fed by hand at 3% of live weight for 90 days. At the end of the study, feeding with oregano EO supplementation did not significantly affect the growth performance of fish. Except for pepsin, there were no significant differences in the activity of digestive enzymes among the control and oregano EO groups. Besides, oregano EO at the doses of 50 or 400 mg kg−1 may have the potential to increase the surface area required for digestion by increasing intestinal villi length. Moreover, all doses of oregano EO showed antimicrobial properties by decreasing the count of lactic acid bacteria in the intestine. Supplementation with 100 and 200 mg kg−1 oregano EO in diets decreased the total coliform, Escherichia coli and lactic acid bacteria counts when compared to the control group. The results showed that oregano EO may positively affect digestion and absorption without adverse effects on the growth performance of Black Sea salmon juvenile.

Evaluation of oregano (<italic>Origanum vulgare</italic>) essential oil supplementation on growth performance, digestive enzymes, intestinal histomorphology and gut microbiota of Black Sea salmon, <italic>Salmo labrax</italic>

Department of Biology, Faculty of Art and Science

Department of Animal Science, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of oregano () essential oil supplementation on growth performance, digestive enzymes, intestinal histomorphology and gut microbiota of Black Sea salmon, 

This study aimed to determine the effect of dietary oregano (Origanum vulgare) essential oil (EO) on the growth performance, digestive enzyme activity,...

Evaluation of oregano (Origanum vulgare) essential oil supplementation on growth performance, digestive enzymes, intestinal histomorphology and gut microbiota of Black Sea salmon, Salmo labrax

Published Online: May 12, 2022

Page range: 763 - 772

Received: May 07, 2021

Accepted: Aug 26, 2021

DOI: https://doi.org/10.2478/aoas-2021-0068

Keywords
aquaculture, phytobiotic, villi, enzyme, bacteria

© 2022 Osman Tolga Özel et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of oregano (Origanum vulgare) essential oil supplementation on growth performance, digestive enzymes, intestinal histomorphology and gut microbiota of Black Sea salmon, Salmo labrax

Published Online: May 12, 2022

Page range: 763 - 772

Received: May 07, 2021

Accepted: Aug 26, 2021

DOI: https://doi.org/10.2478/aoas-2021-0068

Keywordsaquaculture, phytobiotic, villi, enzyme, bacteria

© 2022 Osman Tolga Özel et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
aquaculture, phytobiotic, villi, enzyme, bacteria