<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Most <italic>in vitro</italic> cell-based research is based on two-dimensional (2D) systems where growth and development take place on a flat surface, which does not reflect the natural environment of the cells. The imperfection and limitations of culture in 2D systems eventually led to the creation of three-dimensional (3D) culture models that more closely reproduce the actual conditions of physiological cell growth. Since the inception of 3D culture technology, many culture models have been developed, such as technologies of multicellular spheroids, organoids, and organs on chips in the technology of scaffolding, hydrogels, bio-printing and liquid media. In this review we will focus on the advantages and disadvantages of the 2D vs. 3D cell cultures technologies. We will also try to sum up available 3D culture systems and materials for building 3D scaffolds.</p></abstract>

Most in vitro cell-based research is based on two-dimensional (2D) systems where growth and development take place on a flat surface, which does not reflect the natural environment of the cells. The imperfection and limitations of culture in 2D systems eventually led to the creation of three-dimensional (3D) culture models that more closely reproduce the actual conditions of physiological cell growth. Since the inception of 3D culture technology, many culture models have been developed, such as technologies of multicellular spheroids, organoids, and organs on chips in the technology of scaffolding, hydrogels, bio-printing and liquid media. In this review we will focus on the advantages and disadvantages of the 2D vs. 3D cell cultures technologies. We will also try to sum up available 3D culture systems and materials for building 3D scaffolds.

3D Cell Culture Technology – A New Insight Into <italic>in Vitro</italic> Research – A Review

Department of Reproductive Biotechnology and Cryopreservation

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

3D Cell Culture Technology – A New Insight Into  Research – A Review

Most in vitro cell-based research is based on two-dimensional (2D) systems where growth and development take place on a flat surface, which does not reflect...

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Published Online: Oct 28, 2021

Page range: 1257 - 1273

Received: Nov 19, 2020

Accepted: May 20, 2021

DOI: https://doi.org/10.2478/aoas-2021-0039

Keywords
cell culture models, 3D culture technology, 3D cell culture vs. 2D cell culture, hydrogels, scaffolds

© 2021 Justyna Sośniak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Published Online: Oct 28, 2021

Page range: 1257 - 1273

Received: Nov 19, 2020

Accepted: May 20, 2021

DOI: https://doi.org/10.2478/aoas-2021-0039

Keywordscell culture models, 3D culture technology, 3D cell culture vs. 2D cell culture, hydrogels, scaffolds

© 2021 Justyna Sośniak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
cell culture models, 3D culture technology, 3D cell culture vs. 2D cell culture, hydrogels, scaffolds