<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The development of effective approaches for not only the <italic>in vitro</italic> maturation (IVM) of heifer/cow oocytes and their extracorporeal fertilization (IVF) but also the non-surgical collection and transfer of bovine embryos has given rise to optimizing comprehensive <italic>in vitro</italic> embryo production (IVP) technology and improving other assisted reproductive technologies (ART s), such as cattle cloning by embryo bisection, embryonic cell nuclear transfer (ECNT) and somatic cell nuclear transfer (SCNT). The primary goal of the present paper is to demonstrate the progress and achievements in the strategies utilized for embryonic cell cloning and somatic cell cloning in cattle. Moreover, the current article is focused on recognizing and identifying the suitability and reliability of bovine cloning techniques for nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research and for the genetic rescue of endangered or extinct breeds and species of domesticated or wild-living artiodactyl mammals (even-toed ungulates) originating from the family <italic>Bovidae</italic>.</p>
</abstract>

The development of effective approaches for not only the in vitro maturation (IVM) of heifer/cow oocytes and their extracorporeal fertilization (IVF) but also the non-surgical collection and transfer of bovine embryos has given rise to optimizing comprehensive in vitro embryo production (IVP) technology and improving other assisted reproductive technologies (ART s), such as cattle cloning by embryo bisection, embryonic cell nuclear transfer (ECNT) and somatic cell nuclear transfer (SCNT). The primary goal of the present paper is to demonstrate the progress and achievements in the strategies utilized for embryonic cell cloning and somatic cell cloning in cattle. Moreover, the current article is focused on recognizing and identifying the suitability and reliability of bovine cloning techniques for nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research and for the genetic rescue of endangered or extinct breeds and species of domesticated or wild-living artiodactyl mammals (even-toed ungulates) originating from the family Bovidae.

Generation of Monogenetic Cattle by Different Techniques of Embryonic Cell and Somatic Cell Cloning – Their Application to Biotechnological, Agricultural, Nutritional, Biomedical and Transgenic Research – A Review

Department of Reproductive Biotechnology and Cryoconservation

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The development of effective approaches for not only the in vitro maturation (IVM) of heifer/cow oocytes and their extracorporeal fertilization (IVF) but also...

Generation of Monogenetic Cattle by Different Techniques of Embryonic Cell and Somatic Cell Cloning – Their Application to Biotechnological, Agricultural, Nutritional, Biomedical and Transgenic Research – A Review

Published Online: Aug 05, 2021

Page range: 741 - 755

Received: May 05, 2020

Accepted: Sep 28, 2020

DOI: https://doi.org/10.2478/aoas-2020-0096

Keywords
cattle, cloning, embryo bisection, embryonic cell nuclear transfer, somatic cell nuclear transfer, nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research, endangered and extinct breed or species

© 2021 Maria Skrzyszowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Generation of Monogenetic Cattle by Different Techniques of Embryonic Cell and Somatic Cell Cloning – Their Application to Biotechnological, Agricultural, Nutritional, Biomedical and Transgenic Research – A Review

Published Online: Aug 05, 2021

Page range: 741 - 755

Received: May 05, 2020

Accepted: Sep 28, 2020

DOI: https://doi.org/10.2478/aoas-2020-0096

Keywordscattle, cloning, embryo bisection, embryonic cell nuclear transfer, somatic cell nuclear transfer, nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research, endangered and extinct breed or species

© 2021 Maria Skrzyszowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
cattle, cloning, embryo bisection, embryonic cell nuclear transfer, somatic cell nuclear transfer, nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research, endangered and extinct breed or species