<abstract xmlns="http://www.w3.org/1999/xhtml">This experiment was conducted to establish the effects of two types of microalgae [<italic>Chlorella vulgaris</italic> (AI), <italic>C. variabilis</italic> (AII) and their combination (AI+AII)] with two substrates (wheat and corn silages) on rumen fermentation, gas and methane production. To each substrate, one of 3 algae treatment was supplemented at 0% and 25% of the total incubated dry matter. A series of 5 measurement points (3, 6, 12, 24 and 48 h) were completed and the gas production was monitored. The proximate and mineral composition of microalgae and substrates were examined. At 48 h incubation rumen fermentation variables and CH4 production were also assessed. When compared with wheat silage, corn silage caused an increase in gas production (P&lt;0.05). Ruminal gas production decreased in the algae groups when compared to the controls (0% algae, wheat and corn silages, P&lt;0.05). Among algae, <italic>C. vulgaris</italic> had the strongest effect, decreasing gas production by 34%. Among algae, the total volatile fatty acids (VFA) and CH4 production were found to be lower in <italic>C. variabilis</italic> (P&lt;0.001). Ammonia-N increased with the algae inclusion (P&lt;0.05). But, the ruminal gas production, pH, acetate, the total VFA, CH4 and rumen fermentation efficiency were not affected by the substrate and algae interaction (P>0.05). The propionate was the highest (P&lt;0.05) for corn silage when incubated with <italic>C. vulgaris</italic>. Ruminal butyrate was the lowest for the wheat silage when incubated with the mixture of algae (P&lt;0.05). The NH3-N was the highest in corn silage when incubated with all algae types (P&lt;0.05). Careful selection and combination of substrate and algae may positively manipulate rumen fermentation and may inhibit CH4 production. Further research is needed to validate these results <italic>in vivo</italic>.</abstract>

This experiment was conducted to establish the effects of two types of microalgae [Chlorella vulgaris (AI), C. variabilis (AII) and their combination (AI+AII)] with two substrates (wheat and corn silages) on rumen fermentation, gas and methane production. To each substrate, one of 3 algae treatment was supplemented at 0% and 25% of the total incubated dry matter. A series of 5 measurement points (3, 6, 12, 24 and 48 h) were completed and the gas production was monitored. The proximate and mineral composition of microalgae and substrates were examined. At 48 h incubation rumen fermentation variables and CH4 production were also assessed. When compared with wheat silage, corn silage caused an increase in gas production (P<0.05). Ruminal gas production decreased in the algae groups when compared to the controls (0% algae, wheat and corn silages, P<0.05). Among algae, C. vulgaris had the strongest effect, decreasing gas production by 34%. Among algae, the total volatile fatty acids (VFA) and CH4 production were found to be lower in C. variabilis (P<0.001). Ammonia-N increased with the algae inclusion (P<0.05). But, the ruminal gas production, pH, acetate, the total VFA, CH4 and rumen fermentation efficiency were not affected by the substrate and algae interaction (P>0.05). The propionate was the highest (P<0.05) for corn silage when incubated with C. vulgaris. Ruminal butyrate was the lowest for the wheat silage when incubated with the mixture of algae (P<0.05). The NH3-N was the highest in corn silage when incubated with all algae types (P<0.05). Careful selection and combination of substrate and algae may positively manipulate rumen fermentation and may inhibit CH4 production. Further research is needed to validate these results in vivo.

Effects of Microalgae Species on <italic>In Vitro</italic> Rumen Fermentation Pattern and Methane Production

Department of Animal Science, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of Microalgae Species on  Rumen Fermentation Pattern and Methane Production

This experiment was conducted to establish the effects of two types of microalgae [Chlorella vulgaris (AI), C. variabilis (AII) and their combination (AI+AII)] with two substrates (wheat and corn silages) on rumen fermentation, gas and methane production. To each substrate, one of 3 algae treatment was supplemented at 0% and 25% of the total incubated dry matter. A series of 5 measurement points (3, 6, 12, 24 and 48 h) were completed and the gas production was monitored. The proximate and mineral composition of microalgae and substrates were examined. At 48 h incubation rumen fermentation variables and CH4 production were also assessed. When compared with wheat silage, corn silage caused an increase in gas production (P0.05). The propionate was the highest (P<0.05) for corn silage when incubated with C. vulgaris. Ruminal butyrate was the lowest for the wheat silage when incubated with the mixture of algae (P<0.05). The NH3-N was the highest in corn silage when incubated with all algae types (P<0.05). Careful selection and combination of substrate and algae may positively manipulate rumen fermentation and may inhibit CH4 production. Further research is needed to validate these results in vivo.

This experiment was conducted to establish the effects of two types of microalgae [Chlorella vulgaris (AI), C. variabilis (AII) and their combination (AI+AII)]...

Effects of Microalgae Species on In Vitro Rumen Fermentation Pattern and Methane Production

Published Online: Jan 28, 2020

Page range: 207 - 218

Received: Dec 13, 2018

Accepted: Sep 12, 2019

DOI: https://doi.org/10.2478/aoas-2019-0061

Keywords
microalgae, methane production, rumen fermentation pattern

© 2020 Ekin Sucu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of Microalgae Species on In Vitro Rumen Fermentation Pattern and Methane Production

Published Online: Jan 28, 2020

Page range: 207 - 218

Received: Dec 13, 2018

Accepted: Sep 12, 2019

DOI: https://doi.org/10.2478/aoas-2019-0061

Keywordsmicroalgae, methane production, rumen fermentation pattern

© 2020 Ekin Sucu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
microalgae, methane production, rumen fermentation pattern