<abstract xmlns="http://www.w3.org/1999/xhtml"><p> In the present study, the pedigree and genotype data of 94 individuals were examined for accuracy of parentage allocations and identity test using 12 fluorescent-labelled microsatellite markers in a highly sensitive and accurate ABI system. The panel of 12 markers showed mean PIC value of 0.71 and Shannon index of 1.65 and 7.58 alleles per locus, which suggests that these markers are highly polymorphic and could be useful for parentage control. Based on scoring allele sizes, a total of 91 alleles were observed within the studied population. The highest and lowest number of alleles was observed for TGLA227 locus and TGLA126 and BM1818 loci, respectively. The pedigree was considered incorrect in seven (35%) out of all the evaluated progeny, as their genotype did not match their parents. Combined EP value obtained for all loci in both parentage and identification analysis was 0.99, which indicates the high efficiency of the studied marker set and the accuracy of genotyping in ABI systems. Finally, the present findings confirmed the importance of surveying the pedigree structure and efficiency of 12 fluorescent-labelled microsatellite markers in a single multiplex PCR for parentage testing in the sampled Holstein cattle population.</p></abstract>

In the present study, the pedigree and genotype data of 94 individuals were examined for accuracy of parentage allocations and identity test using 12 fluorescent-labelled microsatellite markers in a highly sensitive and accurate ABI system. The panel of 12 markers showed mean PIC value of 0.71 and Shannon index of 1.65 and 7.58 alleles per locus, which suggests that these markers are highly polymorphic and could be useful for parentage control. Based on scoring allele sizes, a total of 91 alleles were observed within the studied population. The highest and lowest number of alleles was observed for TGLA227 locus and TGLA126 and BM1818 loci, respectively. The pedigree was considered incorrect in seven (35%) out of all the evaluated progeny, as their genotype did not match their parents. Combined EP value obtained for all loci in both parentage and identification analysis was 0.99, which indicates the high efficiency of the studied marker set and the accuracy of genotyping in ABI systems. Finally, the present findings confirmed the importance of surveying the pedigree structure and efficiency of 12 fluorescent-labelled microsatellite markers in a single multiplex PCR for parentage testing in the sampled Holstein cattle population.

Validation of Pedigree Relationships Using a Multiplex Microsatellite Marker Assay in Iranian Holstein Cattle / Walidacja rodowodów irańskiego bydła holsztyńskiego przy użyciu analizy multipleks markerów mikrosatelitarnych

Department of Animal Science, Faculty of Agriculture, University of Zanjan, 45371-38791, Zanjan, Iran

Department of Biotechnology, Animal Science Research Institute of Iran, 1483-31585, Karaj, Iran

Department of Animal Science, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, 4111, Karaj, Iran

Department of Genomic, Agricultural Biotechnology Research Institute of Iran, 31535-1897, Karaj, Iran

Annals of Animal Science

In the present study, the pedigree and genotype data of 94 individuals were examined for accuracy of parentage allocations and identity test using 12...

Validation of Pedigree Relationships Using a Multiplex Microsatellite Marker Assay in Iranian Holstein Cattle / Walidacja rodowodów irańskiego bydła holsztyńskiego przy użyciu analizy multipleks markerów mikrosatelitarnych

Published Online: Aug 01, 2013

Page range: 481 - 493

DOI: https://doi.org/10.2478/aoas-2013-0042

This content is open access.