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<p>In this paper, the synthesis of ginsenoside Rh2 was catalyzed by using heterologous expression of Bacillus subtilis SL-44 glycosyltransferase. The synthesis parameters of ginsenoside Rh2 were optimized by the selection of strains and chemical supplies, the establishment of kinetic equations for the respiration rate of UGT enzyme, the effect of storage temperature on the model, and the glycosylation reaction of ginsenoside PPD with UGT. The effect of Rh2 saturation on the thermal denaturation temperature of the protein was analyzed along with the kinetic properties of the enzyme GE02773 (GE03484) while varying the saturation of Rh2. The results showed that the conversion of ginsenoside Rh2 reached 84% at a temperature of 35℃, pH 8, 5% DMSO, 0.4 of M-UDPG, and 1M-PPD in reaction with GE02773. In this paper, we successfully achieved the efficient synthesis of ginsenoside Rh2, which provides new ways and ideas for the application and synthesis of ginsenoside Rh2, with important practical significance and scientific value.</p>
</abstract>

In this paper, the synthesis of ginsenoside Rh2 was catalyzed by using heterologous expression of Bacillus subtilis SL-44 glycosyltransferase. The synthesis parameters of ginsenoside Rh2 were optimized by the selection of strains and chemical supplies, the establishment of kinetic equations for the respiration rate of UGT enzyme, the effect of storage temperature on the model, and the glycosylation reaction of ginsenoside PPD with UGT. The effect of Rh2 saturation on the thermal denaturation temperature of the protein was analyzed along with the kinetic properties of the enzyme GE02773 (GE03484) while varying the saturation of Rh2. The results showed that the conversion of ginsenoside Rh2 reached 84% at a temperature of 35℃, pH 8, 5% DMSO, 0.4 of M-UDPG, and 1M-PPD in reaction with GE02773. In this paper, we successfully achieved the efficient synthesis of ginsenoside Rh2, which provides new ways and ideas for the application and synthesis of ginsenoside Rh2, with important practical significance and scientific value.

Heterologous expression of Bacillus subtilis SL-44 glycosyltransferase catalyzed synthesis of ginsenoside Rh2

Shaanxi Key Laboratory of Degradable Biomedical Materials

School of Chemical Engineering, Northwest University

Applied Mathematics and Nonlinear Sciences

This work is licensed under the Creative Commons Attribution 4.0 International License.

In this paper, the synthesis of ginsenoside Rh2 was catalyzed by using heterologous expression of Bacillus subtilis SL-44 glycosyltransferase. The synthesis...

Heterologous expression of Bacillus subtilis SL-44 glycosyltransferase catalyzed synthesis of ginsenoside Rh2

Published Online: Oct 11, 2023

Page range: -

Received: Dec 25, 2022

Accepted: Apr 21, 2023

DOI: https://doi.org/10.2478/amns.2023.2.00595

Keywords
Bacillus subtilis, Glycosyltransferase, Kinetic equation, Ginsenoside Rh2

© 2023 Yufei Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Heterologous expression of Bacillus subtilis SL-44 glycosyltransferase catalyzed synthesis of ginsenoside Rh2

Published Online: Oct 11, 2023

Page range: -

Received: Dec 25, 2022

Accepted: Apr 21, 2023

DOI: https://doi.org/10.2478/amns.2023.2.00595

KeywordsBacillus subtilis, Glycosyltransferase, Kinetic equation, Ginsenoside Rh2

© 2023 Yufei Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Bacillus subtilis, Glycosyltransferase, Kinetic equation, Ginsenoside Rh2