Component | Full name | Function | References | |
---|---|---|---|---|
1 | ERAP1 and ERAP2 |
Endoplasmic Reticulum Aminopeptidase 1 and 2 |
Trimming the antigenic peptides in the endoplasmic reticulum | |
2 | LMP2, LMP7 and LMP10 | Immunoproteasome Low Molecular Weight Proteins 2, 7, and 10 | Processing of proteins to peptides in the cytosol | |
3 | LNPEP | Leucyl and Cystinyl Aminopeptidase | Trimming peptides in the cytosol | |
4 | MHC I | Major Histocompatibility Complex I | Presenting antigens to immune cells on the cell surface | |
5 | Tsn | Tapasin | Bridging the MHC I complex to transporters associated with antigen processing (TAP) | |
6 | TAPBPR | TAP-Binding Protein-Related | Facilitating high-affinity epitope selection and peptide loading in the antigen presentation pathway | |
7 | TAP1 and TAP2 | Transporter associated with Antigen Processing 1 and 2 | Transporting peptide precursors into the endoplasmic reticulum |
Objective | Methods | Results | References | |
---|---|---|---|---|
1 | To investigate whether HLA-G polymorphisms may influence susceptibility to endometriosis and its progression |
- PCR-RFLP - Allelic discrimination methods with TaqMan SNP Genotyping Assays |
- The HLA-G rs1632947:GG genotype was associated with protection against the disease and its severe stages - HLA-G rs1233334:CT protected against progression |
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2 | To investigate an association between HLA-C genotype and the occurrence of endometriosis | - Sequence-based typing method | - The occurrence of HLA-C*03:03*01 was increased in endometriosis than in control groups | |
3 | To study the association between HLA genotypes and endometriosis | - PCR-MPH method | - Significant positive association with endometriosis was observed for HLA-B7 | |
4 | To evaluate the sHLA-G levels in the blood sera of women with deep endometriosis and ovarian endometrioma throughout the menstrual cycle and to compare with the levels of sHLA-G in the blood sera of women with ovarian cancer | - ELISA test | - The level of sHLA-G concentration in the blood serum of patients with deep endometriosis fluctuates throughout the menstrual cycle, and during the proliferative and secretory phases, it remains at a high level comparable to that found in patients with ovarian cancer | |
5 | To assess whether the HLA-G is involved in the pathophysiology of endometriosis or disease progression |
- ELISA test - Immunohistochemistry assays |
- Higher concentrations of sHLA-G in the serum but not in the peritoneal fluid were observed in women with advanced endometriosis compared to the control group - |
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6 | To assess whether HLA class I expression on eutopic and ectopic endometrial cells modifies susceptibility to lymphocyte-mediated lysis |
- Immunofluorescence - Flow cytometry |
- The HLA-B7 allele inhibits the cytotoxic activity, suggesting that the growth of ectopic endometrial cells might be under a genetic control | |
7 | To compare the expression of HLA class I in endometrial samples from patients with and without endometriosis | - Immunohistochemical assays | - A significantly higher expression of HLA I in the endometriosis group than in controls, both in the glandular cells and in the stromal cells, was observed | |
8 | To explore the role of DNA methylation in endometriosis |
- Direct bisulfite sequencing - qRT-PCR |
- DNA hypermethylation in the intron VII of the HLA-C*07 gene appears to regulate the expression of HLA-C*07 - The aberrant DNA methylation in this region was positively correlated with the occurrence of endometriosis |