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Figure 1

Light microscopy of testicular tissue in the experimental groups (H&E, 20x). (A) normal seminiferous tubular morphology in control rats; (B) damaged testicular structure in dimethoate-treated rats (arrow head shows the lumens of seminiferous tubules containing fewer elongated spermatids; arrows show haemorrhage; asterisks show desquamation in germinal cells); (C) normal testis structure in rats treated with cherry laurel extract (CLE); (D) histology of the testicular tissue of rats pre-treated with CLE before receiving dimethoate; (E) normal seminiferous tubule morphology in the vitamin C-treated rats receiving dimethoate; (F) rare degenerated tubules and histopathological changes in rats receiving post-treatment with CLE
Light microscopy of testicular tissue in the experimental groups (H&E, 20x). (A) normal seminiferous tubular morphology in control rats; (B) damaged testicular structure in dimethoate-treated rats (arrow head shows the lumens of seminiferous tubules containing fewer elongated spermatids; arrows show haemorrhage; asterisks show desquamation in germinal cells); (C) normal testis structure in rats treated with cherry laurel extract (CLE); (D) histology of the testicular tissue of rats pre-treated with CLE before receiving dimethoate; (E) normal seminiferous tubule morphology in the vitamin C-treated rats receiving dimethoate; (F) rare degenerated tubules and histopathological changes in rats receiving post-treatment with CLE

Figure 2

Apoptotic germ cells (arrows) in testicular tissues (H&E, 400x). (A) the control group; (B) in the dimethoate alone group, many TUNEL-positive apoptotic cells were found; (C) cherry laurel extract (CLE) alone group; (D) CLE pre-treatment decreased the number of TUNEL-positive apoptotic cells in rats receiving dimethoate; (E) vitamin C-treated group receiving dimethoate; (F) CLE post-treatment group receiving dimethoate
Apoptotic germ cells (arrows) in testicular tissues (H&E, 400x). (A) the control group; (B) in the dimethoate alone group, many TUNEL-positive apoptotic cells were found; (C) cherry laurel extract (CLE) alone group; (D) CLE pre-treatment decreased the number of TUNEL-positive apoptotic cells in rats receiving dimethoate; (E) vitamin C-treated group receiving dimethoate; (F) CLE post-treatment group receiving dimethoate

Effects of different treatments on relative reproductive organ weights in rats

Groups Relative reproductive organ weights*
Testis (Right+left/2) Whole epididymis (Right+left/2) Right cauda epididymis Seminal vesicle Ventral prostate
Control 0.433±0.009bc 0.167±0.005ab 0.065±0.001ab 0.369±0.017b 0.176±0.007a
Dimethoate 0.379±0.014d 0.113±0.007c 0.047±0.003c 0.328±0.012c 0.129±0.005b
CLE 0.456±0.013abc 0.171±0.006a 0.069±0.002a 0.408±0.014ab 0.184±0.010a
CLEpre + dimethoate 0.474±0.041ab 0.171±0.005a 0.064±0.001ab 0.448±0.014a 0.166±0.003a
Vitamin C + dimethoate 0.477±0.016a 0.172±0.004a 0.066±0.001ab 0.451±0.007a 0.175±0.003a
Dimethoate + CLEpost 0.430±0.014c 0.146±0.002b 0.060±0.001b 0.388±0.004b 0.166±0.003a
Median 0.442 0.161 0.063 0.390 0.165
First quartile 0.417 0.146 0.060 0.375 0.156
Third quartile 0.469 0.174 0.068 0.443 0.183
Interquartile range 0.060 0.028 0.008 0.063 0.025

Effects of different treatments on whole body and absolute reproductive organ weights in rats

Groups Body (weight g) Absolute reproductive organ weights (g)
Testis (Right+left/2) Whole epididymis (Right+left/2) Right cauda epididymis Seminal vesicle Ventral prostate
Control 310.30±6.19a 1.339±0.003a 0.516±0.035a 0.202±0.001a 1.137±0.039a 0.544±0.019a
Dimethoate 233.75±7.64b 0.878±0.012d 0.260±0.010d 0.111±0.007d 0.761±0.007d 0.298±0.003d
CLE 308.00±9.22a 1.394±0.021a 0.521±0.002a 0.210±0.001a 1.245±0.002b 0.562±0.025a
CLEpre + dimethoate 243.67±4.79b 1.151±0.027b 0.416±0.008b 0.157±0.001b 1.090±0.025a 0.402±0.002bc
Vitamin C + dimethoate 253.70±2.51b 1.209±0.003b 0.437±0.008b 0.168±0.002b 1.142±0.009a 0.442±0.007b
Dimethoate + CLEpost 235.44±2.27b 1.012±0.030c 0.344±0.005c 0.139±0.001c 0.913±0.010c 0.374±0.002c
Median 254.50 1.205 0.430 0.163 1.115 0.415
First quartile 235.22 1.015 0.343 0.139 0.917 0.375
Third quartile 303.00 1.340 0.520 0.203 1.240 0.540
Interquartile range 67.89 0.33 0.18 0.06 0.29 0.16

Effects of different treatments on malondialdehyde (MDA) levels and antioxidant enzyme activities in testicular tissue of rats

Groups Parameters
MDA (nmol/mg protein) SOD (U/mg protein) GPx (U/mg protein) CAT (U/mg protein)
Control 0.341±0.021x 3.676±0.105a 0.331±0.009A 5.257±0.250
Dimethoate 0.486±0.021y 3.174±0.090b 0.265±0.012B 4.291±0.244
CLE 0.386±0.017x 3.635±0.078a 0.335±0.017A 4.068±0.282
CLEpre + dimethoate 0.377±0.015x 3.603±0.066a 0.326±0.014A 4.681±0.310
Vitamin C + dimethoate 0.362±0.012x 3.662±0.079a 0.327±0.014A 4.828±0.257
Dimethoate + CLEpost 0.367±0.028x 3.614±0.114a 0.329±0.017A 5.114±0.294
Median 0.380 3.650 0.320 4.700
First quartile 0.330 3.320 0.290 3.945
Third quartile 0.430 3.805 0.355 5.360
Interquartile range 0.10 0.49 0.07 1.42

Effects of different treatments on semen parameters, DNA damage, and testicular apoptosis in rats

Semen parameters
Groups Motility (%) Concentration (million/right cauda epididymis) Abnormal sperm rate (%) Sperm DNA damage (%) Testicular apoptosis (%)
Head Tail Total
Control 74.34±1.09b 110.40±2.04b 3.60±0.40d 6.10±0.43c 9.70±0.52d 8.47±0.10c 1.57±0.28c
Dimethoate 31.25±2.52e 46.25±1.59e 22.63±0.42a 20.13±0.72a 42.76±0.96a 11.36±0.18a 6.07±0.42a
CLE 83.17±1.28a 121.40±1.08a 3.30±0.30d 5.20±0.39c 8.50±0.37d 8.98±0.13c 1.86±0.30c
CLEpre + dimethoate 54.44±1.39cd 91.78±1.35c 7.67±0.44c 8.78±0.40b 16.45±0.56c 10.12±0.14b 2.70±0.36b
Vitamin C + dimethoate 56.67±1.31c 94.11±1.95c 8.33±0.58c 9.22±0.52b 17.55±0.90bc 10.82±0.16a 2.33±0.40bc
Dimethoate + CLEpost 50.19±1.07d 77.11±1.14d 11.33±0.54b 8.89±0.43b 20.22±0.63b 11.23±0.14a 2.17±0.53bc
Median 55.00 94.00 8.00 8.00 16.00 10.00 1.00
First quartile 50.00 76.28 4.00 6.00 10.00 7.00 0.00
Third quartile 75.84 112.00 11.00 10.00 20.16 13.00 5.00
Interquartile range 26.25 35.72 7.00 4.00 10.16 6.00 5.00
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Journal Subjects:
Medicine, Basic Medical Science, other