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Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
Open Access
Nephrotoxicity and genotoxicity of silver nanoparticles in juvenile rats and possible mechanisms of action
Ye Liu
Ye Liu
,
Li Sun
Li Sun
,
Guili Yang
Guili Yang
and
Zhuo Yang
Zhuo Yang
| Jun 29, 2020
Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
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Article Category:
Original article
Published Online:
Jun 29, 2020
Page range:
121 - 129
Received:
Nov 01, 2019
Accepted:
May 01, 2020
DOI:
https://doi.org/10.2478/aiht-2020-71-3364
Keywords
alkaline comet assay
,
inflammation
,
oxidative stress
,
trpc6 cation channel
© 2020 Ye Liu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1
Transition electron microscopy of silver nanoparticles in suspension
Figure 2
Experimental design of AgNP administration, sampling, and tissue collection; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay
Figure 3
Mean (±SEM) body weight (A), urinary microalbumin (B), serum creatinine (C), tail intensity (comet assay) (D), the frequency of micronucleated PCEs in bone marrow (E), and the PCE to NCE ratio (F) in control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; AgNP – silver nanoparticles; NCE – normochromatic erythrocytes; PCE – polychromatic erythrocytes; SEM – standard error of the mean
Figure 4
Mean (±SEM) ROS GSH, SOD IL-6, IL-1β, and TNF-α in kidney cells and tissue of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; GSH – glutathione; IL – interleukin; ROS – reactive oxygen species; SEM – standard error of the mean; SOD – superoxide dismutase; TNF – tumour necrosis factor
Figure 5
Mean (±SEM) NOX4 and TRPC6 in rat renal cortex (normalised to β-actin expression) of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; NOX – NADPH oxidase 4; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6
Figure 6
TRPC6 immunofluorescence staining of the renal cortex (50 μm scale bar) (A) and mean (±SEM) fluorescent intensity (B) in the glomeruli of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6
Figure 7
Histological findings in the kidney of juvenile rats: (A) control group, (B) 1-wk-AgNP group (C) 2-wk- AgNP group; (D) 3-wk-AgNP group (100 μm scale bar; arrows indicate typically affected areas)
Figure 8
Diagram of the main likely mechanisms of AgNP nephrotoxicity based on oxidative stress; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay; GSH – glutathione; ROS – reactive oxygen species; SOD – superoxide dismutase; TRPC6– transient receptor potential cation channel subfamily C member 6
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