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Introduction. Extended-spectrum β-lactamase (ESβL)-producing <italic>Klebsiella pneumoniae</italic> is currently one of the most common causes of nosocomial infections worldwide. The study aimed to characterize antibiotic resistance profile, the prevalence of selected genes encoding ESβLs, virulence, and the genetic relationship in 139 <italic>K. pneumoniae</italic> isolates identified in John Paul II Specialist Hospital in Southern Poland, collected in 2016.
Materials/Methods. Bacterial identification and the preliminary antibiotic susceptibilities was performed using the VI-TEK® 2 Compact automated system. Genes encoding ESβLs were amplified by CTX-Mplex PCR and PCR reactions. The presence of nine genes encoding virulence factors was studied by multiplex PCR. Clonality was investigated by PFGE after digestion with <italic>Spe</italic>I endonuclease.
Results. <italic>K. pneumoniae</italic> were mostly recovered from the respiratory tract (40.3%), urine (32.4%), wound swabs (19.4%) and blood (5%). In summary, 82.7% of strains were classified as multidrug resistant (MDR). All isolates were confirmed as ESβL producers and carried <italic>bla</italic>CTX-M-type (85.6%), <italic>bla</italic>SHV (82%), <italic>bla</italic>TEM (77.7%), <italic>bla</italic>CTX-M-9 (75.6%) and <italic>bla</italic>CTX-M-1 (1.4%) in various combinations. Moreover, triple <italic>bla</italic> genes were observed in 72% of isolates. The most common virulence-as-sociated genes found among the isolates were <italic>ent</italic>B (91.4%), <italic>ybt</italic>S (55.4%), <italic>iut</italic>A (55.4%), <italic>mag</italic>A (53.2%), <italic>kfu</italic> (14.4%), K2 (11.5%), <italic>mrk</italic>D (10.1%), <italic>rmp</italic>A (7.9%) and <italic>all</italic>S (5%). The PFGE analysis identified 4 major clusters (A–D) comprising 61% of the entire collection.
Conclusions. Our results indicate that the presence of a wide variety of MDR <italic>K. pneumoniae</italic> harbor ESβLs and virulence genes. Studies on molecular epidemiology of ESβL-producing <italic>K. pneumoniae</italic> isolates are needed, particularly for epidemiological surveillance in the hospital environment.
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Introduction. Extended-spectrum β-lactamase (ESβL)-producing Klebsiella pneumoniae is currently one of the most common causes of nosocomial infections worldwide. The study aimed to characterize antibiotic resistance profile, the prevalence of selected genes encoding ESβLs, virulence, and the genetic relationship in 139 K. pneumoniae isolates identified in John Paul II Specialist Hospital in Southern Poland, collected in 2016.
Materials/Methods. Bacterial identification and the preliminary antibiotic susceptibilities was performed using the VI-TEK® 2 Compact automated system. Genes encoding ESβLs were amplified by CTX-Mplex PCR and PCR reactions. The presence of nine genes encoding virulence factors was studied by multiplex PCR. Clonality was investigated by PFGE after digestion with SpeI endonuclease.
Results. K. pneumoniae were mostly recovered from the respiratory tract (40.3%), urine (32.4%), wound swabs (19.4%) and blood (5%). In summary, 82.7% of strains were classified as multidrug resistant (MDR). All isolates were confirmed as ESβL producers and carried blaCTX-M-type (85.6%), blaSHV (82%), blaTEM (77.7%), blaCTX-M-9 (75.6%) and blaCTX-M-1 (1.4%) in various combinations. Moreover, triple bla genes were observed in 72% of isolates. The most common virulence-as-sociated genes found among the isolates were entB (91.4%), ybtS (55.4%), iutA (55.4%), magA (53.2%), kfu (14.4%), K2 (11.5%), mrkD (10.1%), rmpA (7.9%) and allS (5%). The PFGE analysis identified 4 major clusters (A–D) comprising 61% of the entire collection.
Conclusions. Our results indicate that the presence of a wide variety of MDR K. pneumoniae harbor ESβLs and virulence genes. Studies on molecular epidemiology of ESβL-producing K. pneumoniae isolates are needed, particularly for epidemiological surveillance in the hospital environment.

Studies on molecular epidemiology of ESβL-producing <italic>Klebsiella pneumoniae</italic> isolated from patients hospitalized in a specialist hospital in southern Poland

Department of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine

Center for Innovative Laboratory Diagnostics, John Paul II Hospital

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Studies on molecular epidemiology of ESβL-producing  isolated from patients hospitalized in a specialist hospital in southern Poland

Introduction. Extended-spectrum β-lactamase (ESβL)-producing Klebsiella pneumoniae is currently one of the most common causes of nosocomial infections...

Studies on molecular epidemiology of ESβL-producing Klebsiella pneumoniae isolated from patients hospitalized in a specialist hospital in southern Poland

Article Category: Review

Published Online: Dec 30, 2021

Page range: 970 - 979

Received: Dec 02, 2020

Accepted: Aug 09, 2021

DOI: https://doi.org/10.2478/ahem-2021-0039

Keywords
ESβL-producing, MDR, molecular epidemiology, PFGE, virulence-associated genes

© 2021 Brzychczy-Włoch et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Studies on molecular epidemiology of ESβL-producing Klebsiella pneumoniae isolated from patients hospitalized in a specialist hospital in southern Poland

Article Category: Review

Published Online: Dec 30, 2021

Page range: 970 - 979

Received: Dec 02, 2020

Accepted: Aug 09, 2021

DOI: https://doi.org/10.2478/ahem-2021-0039

KeywordsESβL-producing, MDR, molecular epidemiology, PFGE, virulence-associated genes

© 2021 Brzychczy-Włoch et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
ESβL-producing, MDR, molecular epidemiology, PFGE, virulence-associated genes