<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_ahem-2021-0026_s_006"><h3>Objectives</h3>
<p>The article presents the results of an <italic>in vitro</italic> study aimed at identifying changes in parameters of oxidative stress – concentration of malondialdehyde (MDA), enzymatic activity of superoxide dismutase (SOD-1) and protective antioxidant role of melatonin (MLT) during the exposure of blood platelets to electromagnetic radiation (EMR) emitted by monitors.</p></sec>
<sec id="j_ahem-2021-0026_s_007"><h3>Methods</h3>
<p>Platelets were exposed to an EMR for 30- and 60 min. generated by monitors (1 kHz frequency, 220 V/m intensity). In each sample the level of SOD-1 activity and concentration of MDA were determined.</p></sec>
<sec id="j_ahem-2021-0026_s_008"><h3>Results</h3>
<p>The MDA concentration increased significantly after 30-and 60-min. irradiation, as compared to control values (2.53 vs 1.36; 3.64 vs 1.36 nmol/10<sup>9</sup> blood platelets) and after the addition of MLT it decreased (2.53 vs 1.55; 3.64 vs 1.12 nmol/10<sup>9</sup> blood platelets). The activity of SOD-1 increased significantly compared to control values after 30 min. and 60 min. of exposure to EMR (1.97vs 0.75; 2.08 vs 0.75 U/g of protein), and significantly decreased after the addition of MLT only in samples exposed for 60 min. (2.08 vs 0.95 U/g of protein).</p></sec>
<sec id="j_ahem-2021-0026_s_009"><h3>Discussion</h3>
<p>The results demonstrated the possibly negative effect of EMR on oxygen metabolism of blood platelets and indicated a possible protective role of melatonin in this process.</p></sec></abstract>

Objectives
The article presents the results of an in vitro study aimed at identifying changes in parameters of oxidative stress – concentration of malondialdehyde (MDA), enzymatic activity of superoxide dismutase (SOD-1) and protective antioxidant role of melatonin (MLT) during the exposure of blood platelets to electromagnetic radiation (EMR) emitted by monitors.
Methods
Platelets were exposed to an EMR for 30- and 60 min. generated by monitors (1 kHz frequency, 220 V/m intensity). In each sample the level of SOD-1 activity and concentration of MDA were determined.
Results
The MDA concentration increased significantly after 30-and 60-min. irradiation, as compared to control values (2.53 vs 1.36; 3.64 vs 1.36 nmol/109 blood platelets) and after the addition of MLT it decreased (2.53 vs 1.55; 3.64 vs 1.12 nmol/109 blood platelets). The activity of SOD-1 increased significantly compared to control values after 30 min. and 60 min. of exposure to EMR (1.97vs 0.75; 2.08 vs 0.75 U/g of protein), and significantly decreased after the addition of MLT only in samples exposed for 60 min. (2.08 vs 0.95 U/g of protein).
Discussion
The results demonstrated the possibly negative effect of EMR on oxygen metabolism of blood platelets and indicated a possible protective role of melatonin in this process.

The antioxidant effects of melatonin in blood platelets during exposure to electromagnetic radiation – an <italic>in vitro</italic> study

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The antioxidant effects of melatonin in blood platelets during exposure to electromagnetic radiation – an  study

Objectives
The article presents the results of an in vitro study aimed at identifying changes in parameters of oxidative stress – concentration of...

The antioxidant effects of melatonin in blood platelets during exposure to electromagnetic radiation – an in vitro study

Article Category: Review

Published Online: Dec 13, 2021

Page range: 889 - 895

Received: Jul 08, 2020

Accepted: Apr 01, 2021

DOI: https://doi.org/10.2478/ahem-2021-0026

Keywords
electromagnetic radiation, melatonin, malondialdehyde, superoxide dismutase, monitors

© 2021 Lewicka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

The values (median ± standard deviation) of MDA concentration in blood platelets treated with electromagnetic radiation dependent on exposure time and application of melatonin (n=30)

The values (median ± standard deviation) of enzymatic activity of SOD-1 in blood platelets treated with electromagnetic radiation dependent on exposure time and application of melatonin (n=30)

The antioxidant effects of melatonin in blood platelets during exposure to electromagnetic radiation – an in vitro study

Article Category: Review

Published Online: Dec 13, 2021

Page range: 889 - 895

Received: Jul 08, 2020

Accepted: Apr 01, 2021

DOI: https://doi.org/10.2478/ahem-2021-0026

Keywordselectromagnetic radiation, melatonin, malondialdehyde, superoxide dismutase, monitors

© 2021 Lewicka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

The values (median ± standard deviation) of MDA concentration in blood platelets treated with electromagnetic radiation dependent on exposure time and application of melatonin (n=30)

The values (median ± standard deviation) of enzymatic activity of SOD-1 in blood platelets treated with electromagnetic radiation dependent on exposure time and application of melatonin (n=30)

Keywords
electromagnetic radiation, melatonin, malondialdehyde, superoxide dismutase, monitors