<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Aim</h3><p>evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (<italic>Beta vulgaris</italic> (L.) subsp. <italic>vulgaris</italic>) on phenylhydrazine-induced rats.</p></sec>
<sec><h3>Methods</h3><p>Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg<sup>−1</sup> BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg<sup>−1</sup> BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.</p></sec>
<sec><h3>Results</h3><p>The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).</p></sec>
<sec><h3>Conclusion</h3><p>The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.</p></sec>
</abstract>

Aimevaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats.
MethodsMale rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.
ResultsThe results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).
ConclusionThe beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Aimevaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris...

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Article Category: Original Paper

Published Online: Apr 02, 2021

Page range: 1 - 7

Received: Jun 19, 2020

Accepted: Feb 01, 2021

DOI: https://doi.org/10.2478/afpuc-2020-0014

Keywords
Anti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine

© 2021 Ni Putu Ermi Hikmawanti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Article Category: Original Paper

Published Online: Apr 02, 2021

Page range: 1 - 7

Received: Jun 19, 2020

Accepted: Feb 01, 2021

DOI: https://doi.org/10.2478/afpuc-2020-0014

KeywordsAnti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine

© 2021 Ni Putu Ermi Hikmawanti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Anti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine