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Study of the Qualitative Composition and Quantitative Content of Parthenolide in the Feverfew (Tanacetum Parthenium) Herb Cultivated in Ukraine


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Figure 1

TLC of feverfew herb batches and parthenolide reference sample (RS).
TLC of feverfew herb batches and parthenolide reference sample (RS).

Figure 2

Chromatogram of parthenolide reference solution.
Chromatogram of parthenolide reference solution.

Figure 3

Chromatogram of the test solution.
Chromatogram of the test solution.

Figure 4

Graph of linear dependence Yi = b * Xi + a for parthenolide reference solution.
Graph of linear dependence Yi = b * Xi + a for parthenolide reference solution.

Results of quantitative determination of parthenolide in batches of feverfew herb.

Batch No. The region of collection of raw materials Requirements, % Xavr±ΔX, % Criterion satisfaction
Rs 864 Zhytomyr region ≥ 0.2 0.22 ± 0.01 Satisfied
Rs 865 Cherkasy region 0.16 ± 0.06 No satisfied
Rs 866 Sumy region 0.27 ± 0.11 Satisfied
Rs 867 Dnipropetrovsk region 0.26 ± 0.09 Satisfied
Rs 868 Kharkiv region 0.22 ± 0.02 Satisfied
Rs 869 Poltava region 0.24 ± 0.04 Satisfied
Rs 870 Kyiv region 0.39 ± 0.10 Satisfied

The linearity parameters of the quantitative determination method.

Parameter Requirements Received value Criterion satisfaction

|a| ≤ 4.096 0.32 Satisfied
S0 ≤ 2.72 2.57 Satisfied
r > 0.9821 0.9998 Satisfied

Climatic and edaphic conditions of the regions during the growing season of feverfew herb

The region of collection of raw materials Soil Solar insolation, kWh/m2/day Air temperature, °C Precipitation, mm
Zhytomyr region black 3.10–5.21 +21–+26 55–85
Cherkasy region sod-gley / sod-podzolic 3.99–5.54 +20–+25 52–73
Sumy region black 3.12–5.24 +22–+33 50–75
Dnipropetrovsk region black 3.11–5.22 +22–+30 51–74
Kharkiv region black 3.12–5.25 +19–+24 55–85
Poltava region black 3.13–5.23 +21–+26 50–69
Kyiv region black 3.12–5.25 +20–+25 50–70

The results of the evaluation of the accuracy and precision of the HPLC method.

Parameter Index Criterion Criterion satisfaction
Requirements for statistical insignificance Requirements for practical insignificance
0.79 ≤ 1.26% ≤ 2.048% Satisfied
ΔZ 2.83 ≤ 6.4% Satisfied
eISSN:
2453-6725
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Pharmacy, other