<abstract xmlns="http://www.w3.org/1999/xhtml">

Malathion is widely used in agriculture due to their high efficiency as insecticides. They are very toxic hazardous chemicals to both human health and environment even at low concentration. The detection of pesticides (malathion) at the low levels developed by the environmental protection agency (EPA) still remains a challenge. A highly efficient fluorescent biosensor based on g-C3N4/AgNPs for AChE and malathion detection is successfully developed by impregnation method. The structural and morphological properties of the nanocomposites were characterized by using powder X-ray diffraction (XRD), fourier- transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The analysis confirmed that there is a strong interfacial interaction between g-C3N4 and AgNPs. The fluorescent responses show an increase in intensity upon the additions of AChE which indicates that AChE as enzyme was hydrolyzing the substrate ACh, with the increase in oxidative electron as the preferred route of reaction. The developed OFF-ON sensor immobilizes by Actylcholestrase (AChE) and use as new probe for malathion detection. In the absence of malathion, AChE−g-C3N4/AgNCs exhibit high fluorescence intensity. However, the strong interaction of the basic sites to malathion, causes fluorescence quenching via static quenching and Ag form aggregation on the surface of g-C3N4. The experimental parameter such as pH of buffer (pH=6), concentration of acetylcholine (1 mM) and malathion (500 μM) were optimized. The sensor was also more sensitive with Stern-Volmer quenching constants (KSV) of 3.48x10 3 M −1. The practical use of this sensor for malathion determination in Khat was also demonstrated. The obtained amount of malathion in Khat is 168.8 μM.
</abstract>

Malathion is widely used in agriculture due to their high efficiency as insecticides. They are very toxic hazardous chemicals to both human health and environment even at low concentration. The detection of pesticides (malathion) at the low levels developed by the environmental protection agency (EPA) still remains a challenge. A highly efficient fluorescent biosensor based on g-C3N4/AgNPs for AChE and malathion detection is successfully developed by impregnation method. The structural and morphological properties of the nanocomposites were characterized by using powder X-ray diffraction (XRD), fourier- transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The analysis confirmed that there is a strong interfacial interaction between g-C3N4 and AgNPs. The fluorescent responses show an increase in intensity upon the additions of AChE which indicates that AChE as enzyme was hydrolyzing the substrate ACh, with the increase in oxidative electron as the preferred route of reaction. The developed OFF-ON sensor immobilizes by Actylcholestrase (AChE) and use as new probe for malathion detection. In the absence of malathion, AChE−g-C3N4/AgNCs exhibit high fluorescence intensity. However, the strong interaction of the basic sites to malathion, causes fluorescence quenching via static quenching and Ag form aggregation on the surface of g-C3N4. The experimental parameter such as pH of buffer (pH=6), concentration of acetylcholine (1 mM) and malathion (500 μM) were optimized. The sensor was also more sensitive with Stern-Volmer quenching constants (KSV) of 3.48x10 3 M −1. The practical use of this sensor for malathion determination in Khat was also demonstrated. The obtained amount of malathion in Khat is 168.8 μM.

g-C3N4/ Bio–Synthesized Silver Nanoparticle for Fluorometric Bio-Sensing of Acetylcholinesterase and Malathion

Advances in Materials Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

g-CN/ Bio–Synthesized Silver Nanoparticle for Fluorometric Bio-Sensing of Acetylcholinesterase and Malathion

Malathion is widely used in agriculture due to their high efficiency as insecticides. They are very toxic hazardous chemicals to both human health and...

g-C₃N₄/ Bio–Synthesized Silver Nanoparticle for Fluorometric Bio-Sensing of Acetylcholinesterase and Malathion

Published Online: Oct 08, 2022

Page range: 23 - 40

DOI: https://doi.org/10.2478/adms-2022-0011

Keywords
Actylcholestrase, AgNPs, biosensor, fluorescence quenching, malathion, nanoparticle

© 2022 Abdu Hussen Ali, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

g-C3N4/ Bio–Synthesized Silver Nanoparticle for Fluorometric Bio-Sensing of Acetylcholinesterase and Malathion

Published Online: Oct 08, 2022

Page range: 23 - 40

DOI: https://doi.org/10.2478/adms-2022-0011

KeywordsActylcholestrase, AgNPs, biosensor, fluorescence quenching, malathion, nanoparticle

© 2022 Abdu Hussen Ali, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

g-C₃N₄/ Bio–Synthesized Silver Nanoparticle for Fluorometric Bio-Sensing of Acetylcholinesterase and Malathion

Keywords
Actylcholestrase, AgNPs, biosensor, fluorescence quenching, malathion, nanoparticle