<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The utilization of  stored platelet transfusion has emerged as an effective approach in the management of  thrombocytopenia. However, the limited availability of  fresh platelets in veterinary medicine due to the challenging storage requirements poses a significant constraint.  Lyophilized  platelets  offer  extended  shelf-life  and  convenient  storage options, enhancing their accessibility for thrombocytopenic patients. However, only a limited number of  studies have examined the clearance and survival rate of  transfused lyophilized platelets, particularly regarding the lyophilization technique utilizing trehalose as a platelet stabilization agent. The objective of  this study was to assess the recovery and survival rate of  trehalose lyophilized platelets within the circulatory system. To investigate these parameters, CMFDA-labeled lyophilized platelets were administered to mice, and their recovery and survival rates were analyzed. Flow cytometric analysis revealed the rapid clearance of  lyophilized platelets from the systemic circulation. The immediate post-infusion percent recovery of  labeled platelet particles was 42.7 ± 8.15 %. The average survival rates at post-infusion time points at 15, 30, 45, and 60 minutes were 28.2 ± 4.31, 14.5 ± 3.56, 5.1 ± 2.02, and 0.82 ± 0.57, respectively. The calculated mean half-life was 8.39 ± 0.44 minutes. The most pronounced decrease in labeled lyophilized platelet count occurred during the 30-minute timeframe immediately following infusion. Subsequently, over 99% of  lyophilized platelets were eliminated after 60 minutes post-infusion. These findings indicate that higher dosages and more frequent administration of  trehalose lyophilized platelets might be necessary to achieve a therapeutic effect comparable to that of  fresh platelets.</p>
</abstract>

The utilization of  stored platelet transfusion has emerged as an effective approach in the management of  thrombocytopenia. However, the limited availability of  fresh platelets in veterinary medicine due to the challenging storage requirements poses a significant constraint.  Lyophilized  platelets  offer  extended  shelf-life  and  convenient  storage options, enhancing their accessibility for thrombocytopenic patients. However, only a limited number of  studies have examined the clearance and survival rate of  transfused lyophilized platelets, particularly regarding the lyophilization technique utilizing trehalose as a platelet stabilization agent. The objective of  this study was to assess the recovery and survival rate of  trehalose lyophilized platelets within the circulatory system. To investigate these parameters, CMFDA-labeled lyophilized platelets were administered to mice, and their recovery and survival rates were analyzed. Flow cytometric analysis revealed the rapid clearance of  lyophilized platelets from the systemic circulation. The immediate post-infusion percent recovery of  labeled platelet particles was 42.7 ± 8.15 %. The average survival rates at post-infusion time points at 15, 30, 45, and 60 minutes were 28.2 ± 4.31, 14.5 ± 3.56, 5.1 ± 2.02, and 0.82 ± 0.57, respectively. The calculated mean half-life was 8.39 ± 0.44 minutes. The most pronounced decrease in labeled lyophilized platelet count occurred during the 30-minute timeframe immediately following infusion. Subsequently, over 99% of  lyophilized platelets were eliminated after 60 minutes post-infusion. These findings indicate that higher dosages and more frequent administration of  trehalose lyophilized platelets might be necessary to achieve a therapeutic effect comparable to that of  fresh platelets.

Clearance of Trehalose Lyophilized Platelets in Mice

Department of  Small Animal Clinical Sciences

Department of  Veterinary Emergency and Critical Care Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"Clearance of Trehalose Lyophilized Platelets in Mice"}

The utilization of  stored platelet transfusion has emerged as an effective approach in the management of  thrombocytopenia. However, the limited availability...

Clearance of Trehalose Lyophilized Platelets in Mice

Published Online: Dec 14, 2023

Page range: 511 - 518

Accepted: Oct 02, 2023

DOI: https://doi.org/10.2478/acve-2023-0039

Keywords
clearance, lyophilization, mouse, platelet, survival rate, trehalose

© 2023 Mu-Young Kim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Clearance of Trehalose Lyophilized Platelets in Mice

Published Online: Dec 14, 2023

Page range: 511 - 518

Accepted: Oct 02, 2023

DOI: https://doi.org/10.2478/acve-2023-0039

Keywordsclearance, lyophilization, mouse, platelet, survival rate, trehalose

© 2023 Mu-Young Kim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
clearance, lyophilization, mouse, platelet, survival rate, trehalose