<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>Various endogenous and exogenous factors influence the occurrence of  oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of  oxidative stress can lead to changes in the structure of  proteins, lipids, and DNA. The level of  oxidative stress can be determined by measuring the end products of  lipid peroxidation known as reactive substances of  thiobarbituric acid (TBARS) of  which malondialdehyde (MDA) is the most important. The concentration of  MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of  hair sampling.  In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of  lipid peroxidation differed between the sexes, dog breeds, status of  smoking by owner and exposure to UV radiation.</p>
</abstract>

Various endogenous and exogenous factors influence the occurrence of  oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of  oxidative stress can lead to changes in the structure of  proteins, lipids, and DNA. The level of  oxidative stress can be determined by measuring the end products of  lipid peroxidation known as reactive substances of  thiobarbituric acid (TBARS) of  which malondialdehyde (MDA) is the most important. The concentration of  MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of  hair sampling.  In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of  lipid peroxidation differed between the sexes, dog breeds, status of  smoking by owner and exposure to UV radiation.

Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Various endogenous and exogenous factors influence the occurrence of  oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen...

Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study

Published Online: Dec 14, 2023

Page range: 449 - 463

Accepted: Nov 17, 2023

DOI: https://doi.org/10.2478/acve-2023-0034

Keywords
biomarkers, dogs, hair, lipid peroxidation, oxidative stress, ROS

© 2023 Nikolina Boić et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study

Published Online: Dec 14, 2023

Page range: 449 - 463

Accepted: Nov 17, 2023

DOI: https://doi.org/10.2478/acve-2023-0034

Keywordsbiomarkers, dogs, hair, lipid peroxidation, oxidative stress, ROS

© 2023 Nikolina Boić et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
biomarkers, dogs, hair, lipid peroxidation, oxidative stress, ROS