<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The endoplasmic reticulum (ER) is crucial for the production, processing and transport of proteins. Infection with pathogens activates Unfolded Protein Response (UPR), which can lead to their survival/replication or elimination from the body. Although little is known about the role of the ER stress response in the pathogenesis of viral infections, the regulation of ER stress may be important in intractable infectious diseases. We conducted a comparative analysis of the expression of genes involved in ER stress response in peripheral blood mononuclear cells (PBMCs) from animals immunized with an attenuated strain of ASFV strain Congo-a (KK262) and then stimulated <italic>in vitro</italic> by two serologically different virulent strains Congo-v (K49) or Mozambique-v (M78), to expand our understanding of the early determinants of response to homologous and heterologous infection. We found up-regulation of genes of all three sensory molecules (PERK, ATF6 and IRE1) of UPR pathway in cells infected with only a homologous strain. For the first time, a number of up-regulated genes of the ER-associated degradation pathway (ERAD), which destroys misfolded proteins, were also detected. By understanding how viruses modify elements of cellular response to stress, we learn more about the pathogenesis, as well as how we can use it to prevent viral diseases.</p>
</abstract>

The endoplasmic reticulum (ER) is crucial for the production, processing and transport of proteins. Infection with pathogens activates Unfolded Protein Response (UPR), which can lead to their survival/replication or elimination from the body. Although little is known about the role of the ER stress response in the pathogenesis of viral infections, the regulation of ER stress may be important in intractable infectious diseases. We conducted a comparative analysis of the expression of genes involved in ER stress response in peripheral blood mononuclear cells (PBMCs) from animals immunized with an attenuated strain of ASFV strain Congo-a (KK262) and then stimulated in vitro by two serologically different virulent strains Congo-v (K49) or Mozambique-v (M78), to expand our understanding of the early determinants of response to homologous and heterologous infection. We found up-regulation of genes of all three sensory molecules (PERK, ATF6 and IRE1) of UPR pathway in cells infected with only a homologous strain. For the first time, a number of up-regulated genes of the ER-associated degradation pathway (ERAD), which destroys misfolded proteins, were also detected. By understanding how viruses modify elements of cellular response to stress, we learn more about the pathogenesis, as well as how we can use it to prevent viral diseases.

Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections <italic>in vitro</italic>

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections 

The endoplasmic reticulum (ER) is crucial for the production, processing and transport of proteins. Infection with pathogens activates Unfolded Protein...

Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections in vitro

Published Online: Sep 28, 2023

Page range: 405 - 420

Received: May 05, 2023

Accepted: Sep 12, 2023

DOI: https://doi.org/10.2478/acve-2023-0030

Keywords
African swine fever virus, transcriptome, endoplasmic reticulum stress, endoplasmic reticulum-associated degradation pathway

© 2023 Natalia Kholod et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections in vitro

Published Online: Sep 28, 2023

Page range: 405 - 420

Received: May 05, 2023

Accepted: Sep 12, 2023

DOI: https://doi.org/10.2478/acve-2023-0030

KeywordsAfrican swine fever virus, transcriptome, endoplasmic reticulum stress, endoplasmic reticulum-associated degradation pathway

© 2023 Natalia Kholod et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
African swine fever virus, transcriptome, endoplasmic reticulum stress, endoplasmic reticulum-associated degradation pathway