<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In this study, penicillin, oxacillin, and macrolide-lincosamide-streptogramin (MLS) resistance in <italic>S. aureus</italic> strains that were isolated from bovine mastitis cases, and human patients were investigated. Inducible clindamycin resistance (iML) was not found in 30 bovine isolates, while it was detected in 3 (10%) of 30 human isolates. MIC<sub>90</sub> values of penicillin, oxacillin and macrolide-lincosamides (ML) were 2, 0.19, >256 µg/ml in bovine isolates and were 3, 3 and 0.19-1.5 µg/ml in human isolates, respectively. Streptogramin resistance was not found in both bovine and human isolates. Although the <italic>mecA</italic> gene was detected in all of the oxacillin resistant isolates, <italic>blaZ</italic> gene could not be detected in penicillin resistant isolates. The <italic>erm</italic>(<italic>B</italic>) gene was detected in 5 (38.6%) of 13 ML-resistant bovine isolates, and the <italic>mph</italic>(<italic>C</italic>) gene was detected in 2 (66.66%) of 3 human isolates. As a result, resistance to penicillin and oxacillin was found to be higher in human <italic>S. aureus</italic> isolates, while ML resistance was found to be higher in bovine isolates in this investigation. It was concluded that the presence of genes in extra-chromosomal elements associated to penicillin and macrolide resistance should be investigated. The data obtained from this study will contribute to the studies on antimicrobial susceptibility in the field of human and veterinary medicine.</p>
</abstract>

In this study, penicillin, oxacillin, and macrolide-lincosamide-streptogramin (MLS) resistance in S. aureus strains that were isolated from bovine mastitis cases, and human patients were investigated. Inducible clindamycin resistance (iML) was not found in 30 bovine isolates, while it was detected in 3 (10%) of 30 human isolates. MIC90 values of penicillin, oxacillin and macrolide-lincosamides (ML) were 2, 0.19, >256 µg/ml in bovine isolates and were 3, 3 and 0.19-1.5 µg/ml in human isolates, respectively. Streptogramin resistance was not found in both bovine and human isolates. Although the mecA gene was detected in all of the oxacillin resistant isolates, blaZ gene could not be detected in penicillin resistant isolates. The erm(B) gene was detected in 5 (38.6%) of 13 ML-resistant bovine isolates, and the mph(C) gene was detected in 2 (66.66%) of 3 human isolates. As a result, resistance to penicillin and oxacillin was found to be higher in human S. aureus isolates, while ML resistance was found to be higher in bovine isolates in this investigation. It was concluded that the presence of genes in extra-chromosomal elements associated to penicillin and macrolide resistance should be investigated. The data obtained from this study will contribute to the studies on antimicrobial susceptibility in the field of human and veterinary medicine.

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in <italic>Staphylococcus aureus</italic> Isolates from Bovine and Human

Faculty of Veterinary Medicine, Department of Microbiology

Van Yüzüncü Yıl University, Faculty of Veterinary Medicine

Balıkesir University, Faculty of Veterinary Medicine

Istanbul Prof. Dr. Cemil Taşcıoğlu City Hospital, Department of Medical Microbiology

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in  Isolates from Bovine and Human

In this study, penicillin, oxacillin, and macrolide-lincosamide-streptogramin (MLS) resistance in S. aureus strains that were isolated from bovine mastitis...

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

Published Online: Mar 16, 2023

Page range: 102 - 118

Received: Apr 05, 2022

Accepted: Dec 16, 2022

DOI: https://doi.org/10.2478/acve-2023-0008

Keywords
bovine, human, mastitis, MLS resistance,

© 2023 Ozgul Gulaydin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

Published Online: Mar 16, 2023

Page range: 102 - 118

Received: Apr 05, 2022

Accepted: Dec 16, 2022

DOI: https://doi.org/10.2478/acve-2023-0008

Keywordsbovine, human, mastitis, MLS resistance,

© 2023 Ozgul Gulaydin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
bovine, human, mastitis, MLS resistance,