<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics <italic>in vitro</italic>. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.</p>
</abstract>

Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics in vitro. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.

Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)

Sirius University of Science and Technology

Sechenov First Moscow State Medical Univesity

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV)...

Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)

Published Online: Mar 16, 2023

Page range: 13 - 21

Received: Nov 09, 2022

Accepted: Feb 24, 2023

DOI: https://doi.org/10.2478/acve-2023-0002

Keywords
African swine fever virus, multigenic family 110, recombinant virus, COS-1 cell culture

© 2023 Mariia V. Nefedeva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)

Published Online: Mar 16, 2023

Page range: 13 - 21

Received: Nov 09, 2022

Accepted: Feb 24, 2023

DOI: https://doi.org/10.2478/acve-2023-0002

KeywordsAfrican swine fever virus, multigenic family 110, recombinant virus, COS-1 cell culture

© 2023 Mariia V. Nefedeva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
African swine fever virus, multigenic family 110, recombinant virus, COS-1 cell culture