<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress conditions. We investigated how chronic stress isolation (CSI 12 weeks) affected the protein levels of key enzymes involved in adrenaline (A) synthesis (phenyl ethanolamine N-methyltransferase -PNMT), storage (vesicular monoamine transporters 2 - VMAT2) and degradation (catechol-O-methyltransferase - COMT), as well as the concentrations of A as an index for adrenomedullary function in the left and right adrenal medulla. Also, we examined the concentrations of malondialdehyde (MDA), protein levels of nuclear factor κB (NF-κB), and activity of catalase (CAT) in the left and right adrenal medulla. The investigated parameters were quantified by Western blot analysis, assay of enzymatic activity, and CAT Research ELISA kits. We found that CSI pro duced significantly increased levels of PNMT protein, and VMAT2 protein, as well as increased concentrations of A in the right adrenal medulla. However, we recorded that CSI increased protein levels of COMT and NF-κB, as well as the concentrations of MDA in the left adrenal medulla. Also, CSI decreased the activity of CAT only in the left adrenal medulla. Based on these results, it may be concluded that adrenomedullary function is different in respect to the left and right sides in chronic stress conditions.</p>
</abstract>

The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress conditions. We investigated how chronic stress isolation (CSI 12 weeks) affected the protein levels of key enzymes involved in adrenaline (A) synthesis (phenyl ethanolamine N-methyltransferase -PNMT), storage (vesicular monoamine transporters 2 - VMAT2) and degradation (catechol-O-methyltransferase - COMT), as well as the concentrations of A as an index for adrenomedullary function in the left and right adrenal medulla. Also, we examined the concentrations of malondialdehyde (MDA), protein levels of nuclear factor κB (NF-κB), and activity of catalase (CAT) in the left and right adrenal medulla. The investigated parameters were quantified by Western blot analysis, assay of enzymatic activity, and CAT Research ELISA kits. We found that CSI pro duced significantly increased levels of PNMT protein, and VMAT2 protein, as well as increased concentrations of A in the right adrenal medulla. However, we recorded that CSI increased protein levels of COMT and NF-κB, as well as the concentrations of MDA in the left adrenal medulla. Also, CSI decreased the activity of CAT only in the left adrenal medulla. Based on these results, it may be concluded that adrenomedullary function is different in respect to the left and right sides in chronic stress conditions.

Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress

Department of Molecular Biology and Endocrinology

Department of Clinic for Endocrinology, Diabetes and Metabolic Diseases - Laboratory for Human Pancreatic Islets Culture

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress...

Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress

Published Online: Jul 04, 2022

Page range: 224 - 234

Accepted: May 27, 2022

DOI: https://doi.org/10.2478/acve-2022-0018

Keywords
adrenomedullary function, adrenaline, antioxidant status, chronic stress isolation, rats

© 2022 Ljubica Gavrilović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress

Published Online: Jul 04, 2022

Page range: 224 - 234

Accepted: May 27, 2022

DOI: https://doi.org/10.2478/acve-2022-0018

Keywordsadrenomedullary function, adrenaline, antioxidant status, chronic stress isolation, rats

© 2022 Ljubica Gavrilović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
adrenomedullary function, adrenaline, antioxidant status, chronic stress isolation, rats