<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study was aimed to search and characterize the AmpC and/or ESBL genes of <italic>Escherichia coli</italic>, <italic>Klebsiella pneumoniae</italic> and <italic>Pseudomonas aeruginosa</italic> isolated from clinical cases of local livestock and companion animals between 2017 and 2019. A total of eight ceftiofur-resistant <italic>E. coli</italic> (n= 7) and ceftiofur-resistant <italic>K. pneumoniae</italic> (n= 1) and seven <italic>P. aeruginosa</italic> were isolated from different cases in local animals. By combination disc method, six <italic>E. coli</italic> isolates and one <italic>K. pneumoniae</italic> isolate were found to be ESBL producers. By combination of the disc method and double disc synergy test, no <italic>P. aeruginosa</italic> isolates were found as ESBL producers. In the agar disc diffusion test (ADDT) performed with cefoxitin and cefoxitin-boronic, only one <italic>E. coli</italic> was determined as AmpC producer. In ESBL-producing isolates, only the CTX-M class gene was detected by polymerase chain reaction (PCR) and subsequent sequence analysis revealed CTX-M-3 and CTX-M-15 variants. An AmpC positive <italic>E. coli</italic> isolate was found to carry plasmidic <italic>ampC</italic> gene in cmy-2 variant from CIT family. It was observed that <italic>P. aeruginosa</italic> isolates did not carry the plasmidic <italic>ampC</italic> gene. After the chromosomal <italic>ampC</italic> gene of one <italic>P. aeruginosa</italic> was amplified by PCR and sequenced, R79Q and T105A mutations in the chromosomal <italic>ampC</italic> gene was revealed. This showed that overproduction of the <italic>ampC</italic> enzyme is involved in the resistance to β-lactams in <italic>P. aeruginosa</italic> isolates in the study.</p>
</abstract>

This study was aimed to search and characterize the AmpC and/or ESBL genes of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from clinical cases of local livestock and companion animals between 2017 and 2019. A total of eight ceftiofur-resistant E. coli (n= 7) and ceftiofur-resistant K. pneumoniae (n= 1) and seven P. aeruginosa were isolated from different cases in local animals. By combination disc method, six E. coli isolates and one K. pneumoniae isolate were found to be ESBL producers. By combination of the disc method and double disc synergy test, no P. aeruginosa isolates were found as ESBL producers. In the agar disc diffusion test (ADDT) performed with cefoxitin and cefoxitin-boronic, only one E. coli was determined as AmpC producer. In ESBL-producing isolates, only the CTX-M class gene was detected by polymerase chain reaction (PCR) and subsequent sequence analysis revealed CTX-M-3 and CTX-M-15 variants. An AmpC positive E. coli isolate was found to carry plasmidic ampC gene in cmy-2 variant from CIT family. It was observed that P. aeruginosa isolates did not carry the plasmidic ampC gene. After the chromosomal ampC gene of one P. aeruginosa was amplified by PCR and sequenced, R79Q and T105A mutations in the chromosomal ampC gene was revealed. This showed that overproduction of the ampC enzyme is involved in the resistance to β-lactams in P. aeruginosa isolates in the study.

Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals

Department of Microbiology, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

This study was aimed to search and characterize the AmpC and/or ESBL genes of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from...

Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals

Published Online: Dec 30, 2021

Page range: 435 - 450

Accepted: Oct 14, 2021

DOI: https://doi.org/10.2478/acve-2021-0036

Keywords
β-lactamase, dog, Enterobacterales, ruminant

© 2021 Faruk Pehlivanoglu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals

Published Online: Dec 30, 2021

Page range: 435 - 450

Accepted: Oct 14, 2021

DOI: https://doi.org/10.2478/acve-2021-0036

Keywordsβ-lactamase, dog, Enterobacterales, ruminant

© 2021 Faruk Pehlivanoglu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
β-lactamase, dog, Enterobacterales, ruminant