<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, <italic>Ehrlichia canis</italic>. The main vector, a brown-dog tick, <italic>Rhipicephalus sanguineus</italic>, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of <italic>E. canis</italic> among the Macedonian dog population. Up to date, the presence of <italic>E. canis</italic>, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of <italic>E. canis</italic>, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against <italic>E.canis</italic>, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of <italic>E. canis</italic> in pet dogs on the territory of the city of Skopje, Macedonia.</p>
</abstract>

Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.

Molecular Detection of <italic>Ehrlichia canis</italic> in the Pet-Dog Population in R. N. Macedonia

University Ss. Cyril and Methodius - Skopje

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Molecular Detection of  in the Pet-Dog Population in R. N. Macedonia

Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main...

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

Published Online: Jul 12, 2021

Page range: 230 - 238

Received: Apr 12, 2021

Accepted: May 31, 2021

DOI: https://doi.org/10.2478/acve-2021-0020

Keywords
canine monocytic ehrlichiosis, dog, qPCR, 16S rRNA gene

© 2021 Elena Atanaskova Petrov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

Published Online: Jul 12, 2021

Page range: 230 - 238

Received: Apr 12, 2021

Accepted: May 31, 2021

DOI: https://doi.org/10.2478/acve-2021-0020

Keywordscanine monocytic ehrlichiosis, dog, qPCR, 16S rRNA gene

© 2021 Elena Atanaskova Petrov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
canine monocytic ehrlichiosis, dog, qPCR, 16S rRNA gene