1. bookVolume 69 (2019): Issue 4 (December 2019)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds

Published Online: 21 Dec 2019
Volume & Issue: Volume 69 (2019) - Issue 4 (December 2019)
Page range: 434 - 442
Received: 18 Apr 2019
Accepted: 01 Oct 2019
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region.

Keywords

1. Celi P: Biomarkers of oxidative stress in ruminant medicine Immunopharmacol Immunotoxicol 2011;33:233-240.10.3109/08923973.2010.514917Search in Google Scholar

2. Roubies N, Panousis N, Fytianou A, Katsoulos PD, Giadinis N, Karatzias H: Effects of age and reproductive stage on certain serum biochemical parameters of chios sheep under greek rearing conditions. J Vet Med A Physiol Pathol Clin Med 2006;53:277-281.10.1111/j.1439-0442.2006.00832.xSearch in Google Scholar

3. Antunović Z, Senčić Đ, Šperanda M, Liker B: Influence of the season and the reproductive status of ewes on blood parameters. Small Ruminant Res 2002;45:39-44.10.1016/S0921-4488(02)00109-8Search in Google Scholar

4. Lepherd ML, Canfield PJ, Hunt GB, Bosward KL: Haematological, biochemical and selected acute phase protein reference intervals for weaned female merino lambs. Aust Vet J 2009;87:5-11.10.1111/j.1751-0813.2008.00382.x19178470Search in Google Scholar

5. Šimpraga M, Šmuc T, Matanović K, Radin L, Shek-Vugrovečki A, Ljubičić I, Vojta A: Reference intervals for organically raised sheep: Effects of breed, location and season on hematological and biochemical parameters. Small Ruminant Res 2013;112:1-6.10.1016/j.smallrumres.2012.11.032Search in Google Scholar

6. Piccione G, Messina V, Vazzana I, Dara S, Giannetto C, Assenza A: Seasonal variations of some serum electrolyte concentrations in sheep and goats. Comp Clin Pathol 2011;21:911-915.Search in Google Scholar

7. Yokus B, Cakir DU, Kanay Z, Gulten T, Uysal E: Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. J Vet Med A Physiol Pathol Clin Med 2006;53:271-276.10.1111/j.1439-0442.2006.00831.x16901267Search in Google Scholar

8. Oikonomidis IL, Brozos C, Kiossis E, Kritsepi-Konstantinou M: Combined and breed-specific ris for hematologic, biochemical, and hormonal analytes in chios and florina adult rams. Vet Clin Pathol 2018;47:56-68.10.1111/vcp.1255829575144Search in Google Scholar

9. Pinheiro JC, Bates DM: Mixed-effects models in s and s-plus. New York, USA, Springer-Verlag New York, Inc., 2000.10.1007/978-1-4419-0318-1Search in Google Scholar

10. Alila-Johansson A, Eriksson L, Soveri T, Laakso M-L: Serum cortisol levels in goats exhibit seasonal but not daily rhythmicity. Chronobiol Int 2003;20:65-79.10.1081/CBI-12001768412638691Search in Google Scholar

11. Al-Busaidi R, Johnson EH, Mahgoub O: Seasonal variations of phagocytic response, immunoglobulin g (igg) and plasma cortisol levels in dhofari goats. Small Ruminant Res 2008;79:118-123.10.1016/j.smallrumres.2008.07.019Search in Google Scholar

12. Nosaka K, Sakamoto K: Changes in plasma enzyme activity after intramuscular injection of bupivacaine into the human biceps brachii. Acta Physiol Scandinavica 1999;167:259-265.10.1046/j.1365-201x.1999.00612.x10606828Search in Google Scholar

13. Oikonomidis IL, Brozos C, Kiosis E, Kritsepi-Konstantinou M: Hematologic, biochemical, hormonal, and redox status alterations during reproductive activity in adult rams of two breeds. Vet Clin Pathol 2019.10.1111/vcp.1273131062434Search in Google Scholar

14. Aalhus JL, Price MA: The effects of endurance exercise on live animal performance and carcass growth and development in sheep. Can J Anim Sci 1990;70:97-105.10.4141/cjas90-011Search in Google Scholar

15. Webster JR, Moenter SM, Woodfill CJI, Karsch FJ: Role of the thyroid gland in seasonal reproduction. Ii. Thyroxine allows a season-specific suppression of gonadotropin secretion in sheep. Endocrinology 1991;129:176-183.10.1210/endo-129-1-1762055181Search in Google Scholar

16. Menegatos J, Goulas C, Kalogiannis D: The productivity, ovarian and thyroid activity of ewes in an accelerated lambing system in greece. Small Ruminant Res 2006;65:209-216.10.1016/j.smallrumres.2005.06.020Search in Google Scholar

17. Todini L: Thyroid hormones in small ruminants: Effects of endogenous, environmental and nutritional factors. Animal 2007;1:997-1008.10.1017/S175173110700026222444802Search in Google Scholar

18. Zamiri MJ, Khodaei HR: Seasonal thyroidal activity and reproductive characteristics of iranian fat-tailed rams. Anim Reprod Sci 2005;88:245-255.10.1016/j.anireprosci.2004.12.00516143215Search in Google Scholar

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