1. bookVolume 69 (2019): Issue 3 (September 2019)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

NTS Targeted By miR-182 Modulates the Apoptosis of Goat Endometrial Epithelial Cells

Published Online: 24 Sep 2019
Volume & Issue: Volume 69 (2019) - Issue 3 (September 2019)
Page range: 340 - 347
Received: 23 Jan 2019
Accepted: 01 Jul 2019
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Neurotensin is a tridecapeptide that functions in prenatal metabolism. It is targeted and downregulated by miR-182 through binding the seed site of miR-182 to the 3′ untranslated region and reduced by a candidate tumor suppressor, testin. Considering that apoptosis is critical in the development of mammalian preattachment embryos, this study investigated the modulation of neurotensin to the apoptosis of goat endometrial epithelial cells and apoptosis-related proteins (P38 MAPK and caspase8). Results showed that Neurotensin resisted the apoptosis of goat endometrial epithelial cells through the caspase8 pathway and activated the phosphorylation of P38 MAPK, which is involved in blastocyst formation. Thus, miR-182 is likely to promote uterus health by targeting Neurotensin and upregulating Testin.

Keywords

1. J. Lee, Y.D. Kim, C.G. Park, M.Y. Kim, I.Y. Chang, D.C. Zuo, P.K. Shahi, S. Choi, C.H. Yeum, J.Y. Jun: Neurotensin modulates pacemaker activity in interstitial cells of Cajal from the mouse small intestine, Mol. Cells 33 (2012) 509-516.Search in Google Scholar

2. G.W. Bennett, S.H. Moss, C.D. Forster, C.A. Marsden: Developmental changes in neurotensin and its metabolites in the neonatal rat, Developmental Brain Research 111 (1998) 189-196.Search in Google Scholar

3. T.A. Gudasheva, R.U. Ostrovskaya, S.B. Seredenin: Novel technologies for dipeptide drugs design and their implantation, Curr. Pharm. Des. (2018).10.2174/1381612824666181008105641630255630295186Search in Google Scholar

4. R.R. Levy, H. Cordonier, J.C. Czyba, J.F. Guerin: Apoptosis in preimplantation mammalian embryo and genetics, Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia 106 (2001) 101-108.Search in Google Scholar

5. Y. Zhang, L. Zhang, C. Yu, X. Du, X. Liu, J. Liu, X. An, J. Wang, Y. Song, G. Li, B. Cao: Effects of interferon tau on endometrial epithelial cells in caprine in vitro, Gene expression patterns : GEP 25-26 (2017) 142-148.Search in Google Scholar

6. H.Y. Li, S.P. Chang, C.C. Yuan, H.T. Chao, H.T. Ng, Y.J. Sung: Induction of p38 mitogen-activated protein kinase-mediated apoptosis is involved in outgrowth of trophoblast cells on endometrial epithelial cells in a model of human trophoblast-endometrial interactions, Biol. Reprod. 69 (2003) 1515-1524.Search in Google Scholar

7. C.E. Bell, A.J. Watson: p38 MAPK Regulates Cavitation and Tight Junction Function in the Mouse Blastocyst, PLoS One 8 (2013).10.1371/journal.pone.0059528361717323593143Search in Google Scholar

8. P. Juo, C.J. Kuo, J. Yuan, J. Blenis: Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade, Curr. Biol. 8 (1998) 1001.Search in Google Scholar

9. V. Ambros: The functions of animal microRNAs, Nature 431 (2004) 350-355.Search in Google Scholar

10. G. Steponaitis, A. Kazlauskas, D. Skiriute, I. Valiulyte, K. Skauminas, A. Tamasauskas, P. Vaitkiene: Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma, Oncol. Lett. 12 (2016) 3305-3311.Search in Google Scholar

11. R. Dong, H. Pu, Y. Wang, J. Yu, K. Lian, C. Mao: TESTIN was commonly hypermethylated and involved in the epithelial-mesenchymal transition of endometrial cancer, APMIS 123 (2015) 394-400.Search in Google Scholar

12. Z. Zhong, F. Zhang, S.-C. Yin: Effects of TESTIN gene expression on proliferation and migration of the 5-8F nasopharyngeal carcinoma cell line, Asian Pacific journal of cancer prevention : APJCP 16 (2015) 2555-2559.Search in Google Scholar

13. H. Li, K. Huang, L. Gao, L. Wang, Y. Niu, H. Liu, Z. Wang, L. Wang, G. Wang, J. Wang: TES inhibits colorectal cancer progression through activation of p38, Oncotarget 7 (2016) 45819-45836.Search in Google Scholar

14. Z. Gu, G. Ding, K. Liang, H. Zhang, G. Guo, L. Zhang, J. Cui: TESTIN suppresses tumor growth and invasion via manipulating cell cycle progression in endometrial carcinoma, Med. Sci. Monit. 20 (2014) 980-987.Search in Google Scholar

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