Looking for the causes of and solutions to the issue of honey bee colony losses

1. Gallai N, Salles JM, Settele J, Vaissière BE: Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol Econ 2009, 68:810-821.10.1016/j.ecolecon.2008.06.014Search in Google Scholar

2. Moritz RFA, de Miranda J, Fries I, Le Conte Y, Neumann P, Paxton R: Research strategies to improve honeybee health in Europe. Apidologie 2010, 41:227–242.10.1051/apido/2010010Search in Google Scholar

3. vanEngelsdorp D, Meixner MD: A historyical review of managed honey bee populations in Europe and the United States and the factors that may affect them. J Invertebr Pathol 2010, 103:S80-S95.10.1016/j.jip.2009.06.01119909973Search in Google Scholar

4. Neumann P, Carreck NL: Honey bee colony losses. J Apicult Res 2010, 49:1-6.10.3896/IBRA.1.49.1.01Search in Google Scholar

5. Sanchez-Bayo F, Goka K: Pesticide residues and bees - a risk assessment. PLoS ONE 2014, 9:e94482.10.1371/journal.pone.0094482398181224718419Search in Google Scholar

6. Goulson D, Nicholls E, Botías C, Rotheray EL: Bee declines driven by combined stress from parasites, pesticides, and lack of flowers. Science 2015, 347:1255957.10.1126/science.125595725721506Search in Google Scholar

7. McMenamin AJ, Genersch E: Honey bee colony losses and associated viruses. Curr Opin Insect Sci, 2015, 8:121–129.10.1016/j.cois.2015.01.01532846659Search in Google Scholar

8. Sanchez-Bayo F, Goulson D, Pennacchio F, Nazzi F, Goka K, Desneux N: Are bee diseases linked to pesticides? - A brief review. Environ Int 2016, 89:7-11.10.1016/j.envint.2016.01.00926826357Search in Google Scholar

9. FAO - Food and Agriculture Organization of the United Nations: Pollinators vital to our food supply under threat. 2016, http://www.fao.org/news/story/en/item/384726/icode/.Search in Google Scholar

10. Kulhanek K, Steinhauer N, Rennich K, Caron DM, Sagili RR, Pettis JS, Ellis JD, Wilson ME, Wilkes JT, Tarpy DR, Rose R, Lee K, Rangel J, vanEngelsdorp D. A national survey of managed honey bee 2015–2016 annual colony losses in the USA. J Apicult Res 2017, 56:328-340.10.1080/00218839.2017.1344496Search in Google Scholar

11. Ellis JD, Evans JD, Pettis J: Colony losses, managed colony population decline, and colony collapse disorder in the United States. J Apicult Res 2010, 49:134-136.10.3896/IBRA.1.49.1.30Search in Google Scholar

12. Jacques A, Laurent M, EPILOBEE Consortium, Ribière-Chabert M, Saussac M, Bougeard S, Budge GE, Hendrikx P, Chauzat M: A pan-European epidemiological study reveals honey bee colony survival depends on beekeeper education and disease control. PLoS ONE 2017, 12:e0172591.10.1371/journal.pone.0172591534435228278255Search in Google Scholar

13. Brodschneider R, Gray A, Adjlane N, Ballis A, Brusbardis V, Charrière JD, Chlebo R, Coffey MF, Dahle B, de Graaf DC, Dražić MM, Evans G, Fedoriak M, Forsythe I, Gregorc A, Grzęda U, Hetzroni A, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Medina-Flores CA, Mutinelli F, Raudmets A, Ryzhikov VA, Simon-Delso N, Stevanovic J, Uzunov A, Vejsnæs F, Wöhl S, Zammit-Mangion M, Danihlík J: Multi-country loss rates of honey bee colonies during winter 2016/2017 from the COLOSS survey. J Apicult Res 2018, 57:452-457.10.1080/00218839.2018.1460911Search in Google Scholar

14. vanEngelsdorp D, Evans JD, Saegerman C, Mullin C, Haubruge E, Nguyen BK, Frazier M, Frazier J, Cox-Foster D, Chen Y, Underwood R, Tarpy DR, Pettis JS: Colony collapse disorder: a descriptive study. PLoS ONE 2009, 4:e6481.10.1371/journal.pone.0006481271589419649264Search in Google Scholar

15. Cox-Foster DL, Conlan S, Holmes EC, Palacios G, Evans JD, Moran NA, Quan PL, Briese T, Hornig M, Geiser DM, Martinson V, vanEngelsdorp D, Kalkstein AL, Drysdale A, Hui J, Zhai J, Cui L, Hutchison SK, Simons JF, Egholm M, Pettis JS, Lipkin WI: A metagenomic survey of microbes in honey bee colony collapse disorder. Science 2007, 318:283-287.10.1126/science.114649817823314Search in Google Scholar

16. Oldroyd BP: What’s killing American honey bees? PLoS Biol 2007, 5:e168.10.1371/journal.pbio.0050168189284017564497Search in Google Scholar

17. EFSA (European Food Safety Authority): Towards holistic approaches to the risk assessment of multiple stressors in bees. EFSA Scientific Colloquium Summary Report, 15–16 May 2013, Parma, Italy.Search in Google Scholar

18. Lipinski Z, Honey bee nutrition and feeding, in the temperate/continental climate of the northen hemisphere, OZGraf S.A., Olsztyn – A.D., 2018, 1-430.Search in Google Scholar

19. Vanbergen AJ, Insect Pollinators Initiative. Threats to an ecosystem service: pressures on pollinators. Front Ecol Environ 2013, 11:251-259.10.1890/120126Search in Google Scholar

20. Di Pasquale G, Salignon M, Le Conte Y, Belzunces LP, Decourtye A, Kretzschmar A, Suchail, S, Brunet JL, Alaux C. Influence of pollen nutrition on honey bee health: do pollen quality and diversity matter? PLoS ONE 2013, 8:e72016.10.1371/journal.pone.0072016373384323940803Search in Google Scholar

21. Asensio I, Vicente-Rubiano M, Muñoz MJ, Fernández-Carrión E, Sánchez-Vizcaíno JM, Carballo M: Importance of ecological factors and colony handling for optimizing health status of apiaries in Mediterranean ecosystems. PLoS ONE 2016, 11:e0164205.10.1371/journal.pone.0164205505854527727312Search in Google Scholar

22. Stanimirović Z, Soldatović B, Vučinić M: Honey bee – Biology of the bees [in Serbian] Beograd, Srbija: Medicinska knjiga – Medicinske komunikacije 2000, 1-375.Search in Google Scholar

23. Cirkovic D, Stanimirovic Z: Beekeeping manual [in Serbian] Državni univerzitet u Novom Pazaru, Naučna KMD, Beograd, 2018, 1-241.Search in Google Scholar

24. Williams GR, Tarpy DR, vanEngelsdorp D, Chauzat MP, Cox-Foster DL, Delaplane KS, Neumann P, Pettis JS, Rogers ELR, Shutler D: Colony collapse disorder in context. Bioessays 2010, 32:845-846.10.1002/bies.201000075303404120730842Search in Google Scholar

25. Stanimirović Z, Ljubenković J, Stanimirović M, Stevanović J: Eneregetski stres kod medonosne pčele izazvan infekcijom Nosema ceranae. Drugi državni pčelarski sajam, 2010a. Savez Pčelarskih Organizacija Srbije, 41-48.Search in Google Scholar

26. DeGrandi-Hoffman G, Yanping Chen, Huang E, Huang MH. The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.). J Insect Physiol 2010, 56:1184-1191.10.1016/j.jinsphys.2010.03.01720346950Search in Google Scholar

27. Chensheng, LU, Warchol, KM, Callahan RA: Sub-lethal exposure to neonicotinoids impaired honey bees winterization before proceeding to colony collapse disorder. B Insectol 2014, 67:125-130.Search in Google Scholar

28. Wheeler MM, Robinson GE: Diet-dependent gene expression in honey bees: honey vs. sucrose or high fructose corn syrup. Sci Rep 2014, 4:5726.10.1038/srep05726410309225034029Search in Google Scholar

29. Nikolić T, Purać J, Orčić S, Kojić D, Vujanović D, Stanimirović Z, Gržetić I, Ilijević K, Šikoparija B, Blagojević D: Environmental effects on superoxide dismutase and catalase activity and expression in honey bee. Arch Insect Biochem Physiol 2015, 90:181-194.10.1002/arch.2125326314562Search in Google Scholar

30. DeGrandi-Hoffman G, Chen Y: Nutrition, immunity and viral infections in honey bees. Curr Opin Insect Sci 2015, 10:170-176.10.1016/j.cois.2015.05.00729588005Search in Google Scholar

31. Orcic S, Nikolic T, Purac J, Sikoparija B, Blagojević P D, Vukasinovic E, Plavsa N, Stevanovic J, Kojic D: Seasonal variations in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. Entomol Exp Appl 2017, 165:120–128.10.1111/eea.12633Search in Google Scholar

32. Stanimirovic Z, Pejovic D, Stevanovic J, Vucinic M, Mirilovic M: Investigations of hygienic behaviour and disease resistance in organic beekeeping of two honeybee ecogeographic varieties from Serbia. Acta Vet–Beograd 2002, 52:169-180.10.2298/AVB0203169SSearch in Google Scholar

33. Stanimirovic Z, Stevanovic J, Cirkovic D: Behavioural defenses of the honey bee ecotype from Sjenica – Pester against Varroa destructor. Acta Vet-Beograd 2005a, 55:69-82.10.2298/AVB0501069SSearch in Google Scholar

34. Stanimirovic Z, Stevanovic J, Mirilovic M, Stojic V Heritability of hygienic behaviour in grey honey bees (Apis mellifera carnica). Acta Vet-Beograd 2008, 58:593-601.10.2298/AVB0806593SSearch in Google Scholar

35. Stanimirovic Z, Stevanovic J, Aleksic N, Stojic V: Heritability of grooming behaviour in grey honey bees (Apis mellifera carnica). Acta Vet-Beograd 2010b; 60:313-323.10.2298/AVB1003313SSearch in Google Scholar

36. Stanimirović Z, Aleksic N, Stevanovic J, Cirkovic D, Mirilovic M, Djelic N, Stojic V: The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor. Acta Vet-Beograd 2011, 61:309-325.10.2298/AVB1103309SSearch in Google Scholar

37. Stevanovic J: Ecological-ethological defence mechanisms of Apis mellifera carnica against ectoparasite Varroa destructor on the territory of Serbia, PhD dissertation, University of Belgrade, 2007.Search in Google Scholar

38. Pejovic D: Investigation of the heritability of hygienic and grooming behaviour of grey honey bee Apis mellifera carnica and the influence of these features on reproductive and productive traits of honey bee colonies, PhD dissertation, University of Belgrade, 2008.Search in Google Scholar

39. Cirkovic D: The investigation on the effects of pulverised sucrose on the degree of honeybee infestation with Varroa destructor, PhD dissertation, University of Belgrade, 2011.Search in Google Scholar

40. Goblirsch M, Huang ZY, Spivak M: Physiological and behavioral changes in honey bees (Apis mellifera) induced by Nosema ceranae infection. PLoS ONE 2013, 8:e58165.10.1371/journal.pone.0058165359017423483987Search in Google Scholar

41. Stanimirovic Z, Fister S, Stevanovic J: Analysis of sister chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride. Acta Vet-Beograd 2003a, 53:419-425.10.2298/AVB0306419SSearch in Google Scholar

42. Stanimirovic Z, Todorovic D, Stevanovic J, Mladenovic M, Jankovic Lj, Djorđevic M: Influence of cymiazole hydrochloride on mitotic and proliferative activities of cultured human lymphocytes. Acta Vet-Beograd 2003b, 53:47-55.10.2298/AVB0301047SSearch in Google Scholar

43. Stanimirovic Z, Stevanovic J, Jovanovic S, Andjelkovic M: Evaluation of genotoxic effects of Apitol® (cymiazole hydrochloride) in vitro by measurement of sister chromatid exchange. Mutat Res-Gen Tox En 2005b, 588:152-157.10.1016/j.mrgentox.2005.10.00316309949Search in Google Scholar

44. Stanimirovic Z, Stevanovic J, Bajic V, Radovic I: Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. Mutat Res-Gen Tox En 2007a, 628:1-10.10.1016/j.mrgentox.2006.09.01417258933Search in Google Scholar

45. Stanimirovic Z, Pejin II, Kulisic Z, Djiporovic M: Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures. Acta Vet-Beograd 2007b, 57:257-273.10.2298/AVB0703257SSearch in Google Scholar

46. Pejin II, Stanimirović Z, Stevanović J, Kulišić Z: Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo. Veterinarski glasnik 2006, 60:163-173.10.2298/VETGL0604163PSearch in Google Scholar

47. Stevanovic J, Stanimirovic Z, Radakovic M, Stojic V: In vitro evaluation of the clastogenicity of fumagillin. Environ Mol Mutagen 2008, 49:594-601.10.1002/em.2040918613037Search in Google Scholar

48. Radakovic M, Stevanovic J, Djelic N, Lakic N, Knezevic-Vukcevic J, Vukovic-Gacic B, Stanimirovic Z: Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. J Biosciences 2013, 38:53-62.10.1007/s12038-012-9287-223385813Search in Google Scholar

49. Rosenkranz P, Aumeier P, Ziegelmann B: Biology and control of Varroa destructor. J Invertebr Pathol 2010, 103:S96–S119.10.1016/j.jip.2009.07.01619909970Search in Google Scholar

50. Genersch E: Honey bee pathology: current threats to honey bees and beekeeping. Appl Microbiol Biotechnol 2010, 87:87-97.10.1007/s00253-010-2573-820401479Search in Google Scholar

51. Genersch E, von der Ohe W, Kaatz H, Schroeder A, Otten C, Büchler R, Berg S, Ritter W, Mühlen W, Gisder S, Meixner M, Liebig G, Rosenkranz P: The German bee monitoring project: a long term study to understand periodically high winter losses of honey bee colonies. Apidologie 2010, 41:332-352.10.1051/apido/2010014Search in Google Scholar

52. Genersch E, Aubert M: Emerging and re-emerging viruses of the honey bee (Apis mellifera L.). Vet Res 2010 41:54.10.1051/vetres/2010027288314520423694Search in Google Scholar

53. Guzmán-Novoa E, Eccles L, Calvete Y, Mcgowan J, Kelly PG, Correa-Benítez A: Varroa destructor is the main culprit for the death and reduced populations of overwintered honey bee (Apis mellifera) colonies in Ontario, Canada. Apidologie 2010, 41:443-450.10.1051/apido/2009076Search in Google Scholar

54. Ramsey SD, Ochoa R, Bauchan G, Gulbronson C, Mowery JD, Cohen A, Lim D, Joklika J, Cicero JM, Ellis JD, Hawthorne D, vanEngelsdorp D: Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph. P Natl Acad Sci USA 2019, 116:1792-1801.10.1073/pnas.1818371116635871330647116Search in Google Scholar

55. Di Prisco G, Annoscia D, Margiotta M, Ferrara R, Varricchio P, Zanni V, Caprio E, Nazzi F, Pennacchio F: A mutualistic symbiosis between a parasitic mite and a pathogenic virus undermines honey bee immunity and health. P Natl Acad Sci USA, 2016, 113:3203-3208.10.1073/pnas.1523515113481273026951652Search in Google Scholar

56. Zhao Y, Heerman M, Peng W, Evans JD, Rose R, DeGrandi-Hoffman G, Simone-Finstrom M, Li J, Li Z, Cook SC, Su S, Rodríguez-García C, Banmeke O, Hamilton M, Chen Y: The dynamics of deformed wing virus concentration and host defensive gene expression after Varroa mite parasitism in honey bees, Apis mellifera. Insects 2019, 10:16.10.3390/insects10010016635890130626033Search in Google Scholar

57. Alaux C, Dantec C., Parrinello H, Le Conte Y: Nutrigenomics in honey bees: digital gene expression analysis of pollen’s nutritive effects on healthy and varroa-parasitized bees. BMC Genomics 2011, 12:496.10.1186/1471-2164-12-496320967021985689Search in Google Scholar

58. Shen M, Yang X, Cox-Foster D, Cui L: The role of varroa mites in infections of Kashmir bee virus (KBV) and deformed wing virus (DWV) in honey bees. Virology 2005a, 342:141-149.10.1016/j.virol.2005.07.01216109435Search in Google Scholar

59. Shen M, Cui L, Ostiguy N, Cox-Foster D: Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee host and the parasitic varroa mite. J Gen Virol 2005b, 86:2281-2289.10.1099/vir.0.80824-016033976Search in Google Scholar

60. Berényi O, Bakonyi T, Derakhshifar I, Köglberger H, Nowotny N: Occurrence of six honeybee viruses in diseased Austrian apiaries. Appl Environ Microbiol 2006, 72:2414-2420.10.1128/AEM.72.4.2414-2420.2006144902716597939Search in Google Scholar

61. Martin SJ, Highfield AC, Brettell L, Villalobos EM, Budge GE, Powell M, Nikaido S, Schroeder DC: Global honey bee viral landscape altered by a parasitic mite. Science 2012, 336:1304–1306.10.1126/science.122094122679096Search in Google Scholar

62. Nazzi F, Brown SP, Annoscia D, Del Piccolo F, Di Prisco G, Varricchio P, Della Vedova G, Cattonaro F, Caprio E, Pennacchio F: Synergistic parasite-pathogen interactions mediated by host immunity can drive the collapse of honeybee colonies. PLoS Pathog 2012, 8:e1002735.10.1371/journal.ppat.1002735337529922719246Search in Google Scholar

63. Gregorc A, Evans JD, Scharf M, Ellis JD: Gene expression in honey bee (Apis mellifera) larvae exposed to pesticides and Varroa mites (Varroa destructor). J Insect Physiol 2012, 58:1042-1049.10.1016/j.jinsphys.2012.03.01522497859Search in Google Scholar

64. Francis RM, Nielsen SL, Kryger P: Varroa–virus interaction in collapsing honey bee colonies. PLoS ONE, 2013, 8:e57540.10.1371/journal.pone.0057540360252323526946Search in Google Scholar

65. Sabin LR, Hanna SL, Cherry S: Innate antiviral immunity in Drosophila. Curr Opin Immunol 2010, 22:4–9.10.1016/j.coi.2010.01.007283114320137906Search in Google Scholar

66. Nazzi F, Pennacchio F: Disentangling multiple interactions in the hive ecosystem. Trends Parasitol 2014, 30:556–561.10.1016/j.pt.2014.09.00625457396Search in Google Scholar

67. Fine JD, Cox-Foster DL, Mullin CA: An inert pesticide adjuvant synergizes viral pathogenicity and mortality in honey bee larvae. Sci Rep-UK 2017, 7:40499.10.1038/srep40499523842128091574Search in Google Scholar

68. Simeunovic P, Stevanovic J, Vidanovic D, Nisavic J, Radovic D, Stanisic Lj, Stanimirovic Z: A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR. Acta Vet-Beograd 2014a, 64:81-92.10.2478/acve-2014-0009Search in Google Scholar

69. Cirkovic D, Stevanovic J, Glavinic U, Aleksic N, Djuric S, Aleksic J, Stanimirovic Z: Honey bee viruses in Serbian colonies of different strength. PeerJ 2018, 6:e5887.10.7717/peerj.5887624034030479890Search in Google Scholar

70. Taric E, Glavinic U, Stevanovic J, Vejnovic B, Aleksic N, Dimitrijevic V, Stanimirovic Z: Occurrence of honey bee (Apis mellifera L.) pathogens in commercial and traditional hives. J Apicult Res 2019, DOI: 10.1080/00218839.2018.1554231.10.1080/00218839.2018.1554231Search in Google Scholar

71. Glavinic U, Stevanovic J, Gajic B, Simeunovic P, Đuric S, Vejnovic B, Stanimirovic Z: Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. Acta Vet-Beograd 2014, 64:349-357.10.2478/acve-2014-0033Search in Google Scholar

72. Grozinger CM, Flenniken ML: Bee Viruses: Ecology, Pathogenicity, and Impacts. Annu Rev Entomol 2019, 64:205-226.10.1146/annurev-ento-011118-11194230629896Search in Google Scholar

73. Stanimirović Z, Glavinic U, Lakic N, Radovic D, Ristanic M, Taric E, Stevanovic J: Efficacy of plant-derived formulation “Argus Ras” in Varroa destructor control. Acta Vet-Beograd 2017, 67:191-200.10.1515/acve-2017-0017Search in Google Scholar

74. Giacomelli A, Pietropaoli M, Carvelli A, Iacoponi F, Formato G: Combination of thymol treatment (Apiguard®) and caging the queen technique to fight Varroa destructor. Apidologie 2016, 47:606-616.10.1007/s13592-015-0408-4Search in Google Scholar

75. Stanimirović Z, Ćirković D, Pejin II, Pejović D: Strategy for ecologic control in fighting Varroa destructor. Veterinarski glasnik 2007c, 61:11-35.10.2298/VETGL0702011SSearch in Google Scholar

76. Stevanovic J, Stanimirovic Z, Lakic N, Aleksic N, Simeunovic P, Kulisic Z: Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies. Arch Biol Sci 2011a, 63:1199-1207.10.2298/ABS1104199SSearch in Google Scholar

77. Stevanovic J, Stanimirovic Z, Lakic N, Djelic N, Radovic I: Stimulating effect of sugar dusting on honey bee grooming behaviour. Entomol Exp Appl 2012a, 143:23-30.10.1111/j.1570-7458.2012.01231.xSearch in Google Scholar

78. Lodesani M, Costa M: Limits of chemotherapy in beekeeping: development of resistance and the problem of residues. Bee World 2005, 86:102–109.10.1080/0005772X.2005.11417324Search in Google Scholar

79. Loucif-Ayad W, Aribi N, Smagghe G, Soltani N: Comparative effectiveness of some acaricides used to control Varroa destructor (Mesostigmata: Varroidae) in Algeria. Afr Entomol 2010, 18:259–266.10.4001/003.018.0211Search in Google Scholar

80. Kanga LHB, Adamczyk J, Marshall K, Cox R: Monitoring for resistance to organophosphorus and pyrethroid insecticides in Varroa mite populations. J Econ Entomol 2010, 103:1797-1802.10.1603/EC1006421061982Search in Google Scholar

81. Bogdanov S: Contaminants of bee products. Apidologie 2006, 37:1–18.10.1051/apido:2005043Search in Google Scholar

82. Adamczyk S, Lázaro R, Pérez-Arquillué C, Herrera A: Determination of synthetic acaricides residues in beeswax by high-performance liquid chromatography with photodiode array detector. Anal Chim Acta 2007, 581:95–101.10.1016/j.aca.2006.07.08517386431Search in Google Scholar

83. Adamczyk S, Lázaro R, Pérez-Arquillué C, Bayarri S, Herrera A: Impact of the use of fluvalinate on different types of beeswax from Spanish hives. Arch Environ Con Tox 2010, 58:733–739.10.1007/s00244-009-9387-719756841Search in Google Scholar

84. Stevanovic J, Stanimirovic Z, Aleksic N, Simeunovic P, Vucicevic M: The influence of natural and synthetic substances applied in honey bee health care on the quality of bee products (invited paper). Proceedings of the Apimondia Symposium “APIECOTECH SERBIA 2012“, Feb 18-19, 2012b, Belgrade, Serbia, pp 9-34.Search in Google Scholar

85. Bogdanov S, Kolchenmann V, Imdorf A: Acaricide residues in some bee products. J Apicult Res 1998, 37:57-67.10.1080/00218839.1998.11100956Search in Google Scholar

86. Chauzat MP, Faucon JP, Martel AC, Lachaize J, Cougoule N, Aubert M: A survey of pesticide residues in pollen loads collected by honey bees in France. J Econ Entomol 2006, 99:253–262.10.1093/jee/99.2.253Search in Google Scholar

87. Chauzat MP, Faucon JP: Pesticides residues in beeswax samples collected from honey-bee colonies (Apis mellifera) in France. Pest Manag Sci 2007, 63:1100–1106.10.1002/ps.145117879980Search in Google Scholar

88. Frazier M, Mullin C, Frazier J, Ashcraft S: What have pesticides got to do with it? Am Bee J 2008, 148:521–523.Search in Google Scholar

89. Mullin CA, Frazier M, Frazier JL, Ashcraft S, Simonds R, vanEngelsdorp D, Pettis JS: High levels of miticides and agrochemicals in North American apiaries: Implications for honey bee health. PLoS ONE 2010, 5:e9754.10.1371/journal.pone.0009754284163620333298Search in Google Scholar

90. Orantes-Bermejo FJ, Pajuelo AG, Megías MM, Fernández-Píñar C: Pesticide residues in beeswax and beebread samples collected from honey bee colonies (Apis mellifera L.) in Spain. Possible implications for bee losses. J Apicult Res 2010, 48:243-250.10.3896/IBRA.1.49.3.03Search in Google Scholar

91. Chauzat MP, Carpentier P, Martel AC, Bougeard S, Cougoule N, Porta P, Lachaize J, Madec F, Aubert M, Faucon JP: Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environ Entomol 2009, 38:514–523.10.1603/022.038.030219508759Search in Google Scholar

92. Bevk D, Kralj J, Čokl A: Coumaphos affects food transfer between workers of honeybee Apis mellifera. Apidologie 2012, 43:465–470.10.1007/s13592-011-0113-xSearch in Google Scholar

93. Umpiérrez ML, Santos E, González A, Rossini C: Plant essential oils as potential control agents of varroatosis. Phytochem Rev 2011, 10:227-244.10.1007/s11101-010-9182-0Search in Google Scholar

94. Satta A, Floris I, Eguaras M, Cabras P, Garau VL, Melis M: Formic acid-based treatments for control of Varroa destructor in a Mediterranean area. J Econ Entomol 2005, 98:267-273.10.1093/jee/98.2.26715889712Search in Google Scholar

95. Damiani N, Gende LB, Bailac P, Marcangeli JA, Eguaras MJ: Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae). Parasitol Res 2009, 106:145–152.10.1007/s00436-009-1639-y19795133Search in Google Scholar

96. DeGrandi-Hoffman G, Ahumada F, Probasco G, Schantz, L: The effects of beta acids from hops (Humulus lupulus) on mortality of Varroa destructor (Acari: Varroidae). Exp Appl Acarol 2012, 58:407-421.10.1007/s10493-012-9593-2348700222767150Search in Google Scholar

97. Ebert TA, Kevan PG, Bishop BL, Kevan SD, Downer RA: Oral toxicity of essential oils and organic acids fed to honey bees (Apis mellifera). J Apicult Res 2007, 46:220–224.10.1080/00218839.2007.11101398Search in Google Scholar

98. Ziegelmann B, Abele E, Hannus S, Beitzinger M, Berg S, Rosenkranz P: Lithium chloride effectively kills the honey bee parasite Varroa destructor by a systemic mode of action. Sci Rep-UK 2018, 8:683.10.1038/s41598-017-19137-5576653129330449Search in Google Scholar

99. Forsgren E: European foulbrood in honey bees, J Invertebr Pathol 2010, 103:5-9.10.1016/j.jip.2009.06.01620105559Search in Google Scholar

100. Vojvodic S, Boomsma JJ, Eilenberg J, Jensen AB: Virulence of mixed fungal infections in honey bee brood. Front Zool 2012, 9:5.10.1186/1742-9994-9-5338423622444792Search in Google Scholar

101. Jensen AB, Aronstein K, Flores JM, Vojvodic S, Palacio MA, Spivak M: Standard methods for fungal brood disease research. In: Dietemann V, Ellis JD and Neumann P (Eds.) The COLOSS BEEBOOK, Volume II: Standard methods for Apis mellifera research. J Apicult Res 2013, 52 (1) DOI: 10.3896/IBRA.1.52.1.1310.3896/IBRA.1.52.1.13381665224198438Search in Google Scholar

102. Ashiralieva A, Genersch E: Reclassification, genotypes and virulence of Paenibacillus larvae, the etiological agent of American foulbrood in honeybees – a review. Apidologie 2006, 37:411-420.10.1051/apido:2006028Search in Google Scholar

103. Forsgren E, Stevanovic J, Fries I: Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotype. Vet. Microbiol 2008, 129:342-349.10.1016/j.vetmic.2007.12.00118207338Search in Google Scholar

104. Forsgren E, Budge GE, Charrière JD, Hornitzky MA: Standard methods for European foulbrood research. In: Dietemann V, Ellis JD and Neumann P (Eds.) The COLOSS BEEBOOK, Volume II: Standard methods for Apis mellifera research. J Apicult Res 2013, 52 (1) DOI: 10.3896/IBRA.1.52.1.1210.3896/IBRA.1.52.1.12Search in Google Scholar

105. Wilkins S, Brown M, Andrew A, Cuthbertson GS: The incidence of honey bee pests and diseases in England and Wales. Pest Manag Sci 2007, 63:1062–1068.10.1002/ps.146117879983Search in Google Scholar

106. Roetschi A, Berthoud H, Kuhn R, Imdorf A: Infection rate based on quantitative real-time PCR of Melissococcus plutonius, the causal agent of European foulbrood, in honeybee colonies before and after apiary sanitation. Apidologie 2008, 39:362–371.10.1051/apido:200819Search in Google Scholar

107. Forsgren E, Lundhagen AC, Imdorf A, Fries I: Distribution of Melissococcus plutonius in honeybee colonies with and without symptoms of European foulbrood. Microbial Ecol 2005, 50:369–374.10.1007/s00248-004-0188-216254762Search in Google Scholar

108. Hedtke K, Jensen PM, Jensen AB, Genersch E: Evidence for emerging parasites and pathogens influencing outbreaks of stress-related diseases like chalkbrood. J Invertebr Pathol 2011, 108:167-173.10.1016/j.jip.2011.08.00621906600Search in Google Scholar

109. Aronstein K, Murray KD: Chalkbrood disease in honey bees. J Invertebr Pathol 2010, 103:S20–S29.10.1016/j.jip.2009.06.01819909969Search in Google Scholar

110. Stevanovic J, Stanimirovic Z, Genersch E, Kovacevic RS, Ljubenkovic J, Radakovic M, Aleksic N. Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. Apidologie 2011b, 41:49-58.10.1051/apido/2010034Search in Google Scholar

111. Stevanovic J, Simeunovic P, Gajic B, Lakic N, Radovic D, Fries I, Stanimirovic Z: Characteristics of Nosema ceranae infection in Serbian honey bee colonies. Apidologie 2013, 44:522-536.10.1007/s13592-013-0203-zSearch in Google Scholar

112. Stevanovic J, Schwarz RS, Vejnovic B, Evans JD, Irwin RE, Glavinic U, Stanimirovic Z. Species-specific diagnostics of Apis mellifera trypanosomatids: a nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees. J Invertebr Pathol 2016, 139:6-11.10.1016/j.jip.2016.07.00127392956Search in Google Scholar

113. Schwarz RS, Bauchan GR, Murphy C, Ravoet J, de Graaf DC, Evans JD: Characterization of two species of Trypanosomatidae from the honey bee Apis mellifera: Crithidia mellificae Langridge and McGhee, and Lotmaria passim. J Eukaryot Microbiol 2015a, 62:567-583.10.1111/jeu.1220925712037Search in Google Scholar

114. Vejnovic B, Stevanovic J, Schwarz RS, Aleksic N, Mirilovic M, Jovanovic NM, Stanimirovic Z: Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae. J Invertebr Pathol 2018, 151:76-81.10.1016/j.jip.2017.11.00329113738Search in Google Scholar

115. Martín-Hernández, R, Botías, C, Bailón, E G, Martínez-Salvador, A, Prieto, L, Meana, A, Higes, M: Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis?. Environ Microbiol 2012, 14:2127-2138.10.1111/j.1462-2920.2011.02645.x22176602Search in Google Scholar

116. Higes M, Meana A, Bartolomé C, Botías C, Martín-Hernández R: Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen. Env Microbiol Rep 2013, 5:17–29.10.1111/1758-2229.1202423757127Search in Google Scholar

117. Schwarz RS, Huang Q, Evans JD: Hologenome theory and the honey bee pathosphere. Curr Opin Insect Sci 2015b, 10:1-7.10.1016/j.cois.2015.04.00629587997Search in Google Scholar

118. Martín-Hernández, R., Bartolomé, C., Chejanovsky, N., Le Conte, Y., Dalmon, A., Dussaubat, C., García-Palencia, P., Meana, A., Pinto, M.A., Soroker, V. and Higes, M: Nosema ceranae in Apis mellifera: a 12 years postdetection perspective. Environ Microbiol 2018, 20:1302-1329.10.1111/1462-2920.1410329575513Search in Google Scholar

119. Fries I: Nosema ceranae in European honey bees (Apis mellifera). J Invertebr Pathol 2010, 103:S73–S79.10.1016/j.jip.2009.06.01719909977Search in Google Scholar

120. Gisder S, Hedtke K, Möckel N, Frielitz MC, Linde A, Genersch E: Five-year cohort study of Nosema spp. in Germany: does climate shape virulence and assertiveness of Nosema ceranae? Appl Environ Microb 2010, 76:3032–3038.10.1128/AEM.03097-09286343920228103Search in Google Scholar

121. Gisder S, Schüler V, Horchler LL, Groth D, Genersch E: Long-term temporal trends of Nosema spp. infection prevalence in Northeast Germany: Continuous spread of N. ceranae, an emerging pathogen of honey bees (Apis mellifera), but no general replacement of N. apis. Front Cell Infect Microbiol 2017, 7:301.10.3389/fcimb.2017.00301549848428730143Search in Google Scholar

122. Forsgren E, Fries I: Temporal study of Nosema spp. in a cold climate. Environ Microbiol Rep 2013, 5:78–82.10.1111/j.1758-2229.2012.00386.x23757134Search in Google Scholar

123. Chemurot M, De Smet L, Brunain M, De Rycke R, de Graaf DC: Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda. Eur J Protistol 2017, 61:13–19.10.1016/j.ejop.2017.07.00228826077Search in Google Scholar

124. Martín-Hernández R, Meana A, Prieto L, Salvador AM, Garrido-Bailón E, Higes M. Outcome of colonization of Apis mellifera by Nosema ceranae, Appl Environ Microb 2007, 73:6331–6338.10.1128/AEM.00270-07207503617675417Search in Google Scholar

125. Higes M, Martín-Hernández R, Botías C, Garrido Bailón E, González-Porto AV, Barrios L, Del Nozal MJ, Bernal JL, Jiménez JJ, Palencia PG, Meana A: How natural infection by Nosema ceranae causes honeybee colony collapse. Environ Microbiol 2008, 10:2659–2669.10.1111/j.1462-2920.2008.01687.x18647336Search in Google Scholar

126. Higes M, Martín-Hernández R, Garrido-Bailón E, González-Porto AV, García-Palencia P, Meana A, Del Nozal MJ, Mayo R, Bernal JL: Honeybee colony collapse due to Nosema ceranae in professional apiaries. Env Microbiol Rep 2009, 1:110–113.10.1111/j.1758-2229.2009.00014.x23765741Search in Google Scholar

127. Antúnez K, Martín-Hernández R, Prieto L, Meana A, Zunino P, Higes M: Immune suppression in the honey bee (Apis mellifera) following infection by Nosema ceranae (Microsporidia). Environ Microbiol 2009, 11:2284-2290.10.1111/j.1462-2920.2009.01953.x19737304Search in Google Scholar

128. Chaimanee V, Chantawannakul P, Chen Y, Evans YD, Pettis JS: Differential expression of immune genes of adult honey bee (Apis mellifera) after inoculated by Nosema ceranae. J Insect Physiol 2012, 58:1090–1095.10.1016/j.jinsphys.2012.04.01622609362Search in Google Scholar

129. Glavinic U, Stankovic B, Draskovic V, Stevanovic J, Petrovic T, Lakic N, Stanimirovic Z: Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. PLoS ONE 2017, 12:e0187726.10.1371/journal.pone.0187726567888729117233Search in Google Scholar

130. Higes M, Martin-Hernandez R, Meana A: Nosema ceranae in Europe: an emergent type C nosemosis. Apidologie 2010, 41:375-392.10.1051/apido/2010019Search in Google Scholar

131. Simeunovic P, Stevanovic J, Cirkovic D, Radojicic S, Lakic N, Stanisic L, Stanimirovic Z: Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. J Apicult Res 2014b, 53:545-554.10.3896/IBRA.1.53.5.09Search in Google Scholar

132. Bacandritsos N, Granato A, Budge G, Papanastasiou I, Roinioti E, Caldon M, Falcaro C, Gallina A, Mutinelli F: Sudden deaths and colony population decline in Greek honey bee colonies. J Invertebr Pathol 2010, 105:335–340.10.1016/j.jip.2010.08.00420804765Search in Google Scholar

133. Bromenshenk JJ, Henderson CB, Wick CH, Stanford MF, Zulich AW, Jabbour RE, Deshpande SV, McCubbin PE, Seccomb RA, Welch PM, Williams T: Iridovirus and microsporidian linked to honey bee colony decline. PLoS ONE 2010, 5:e13181.10.1371/journal.pone.0013181295084720949138Search in Google Scholar

134. Soroker V, Hetzroni A, Yakobson B, David D, David A, Voet H, Slabezki Y, Efrat H, Levski S, Kamer Y, Klinberg E, Zioni N, Inbar S, Chejanovsky N: Evaluation of colony losses in Israel in relation to the incidence of pathogens and pests. Apidologie, 2011, 42:192–199.10.1051/apido/2010047Search in Google Scholar

135. Vidau C, Diogon M, Aufauvre J, Fontbonne R, Viguès B, Brunet JL, Texier C, Biron DG, Blot N, Al Alaoui H, Belzunces LP, Delbac F: Exposure to sublethal doses of fipronil and thiacloprid highly increases mortality of honeybees previously infected by Nosema ceranae. PLoS ONE 2011, 6:e21550.10.1371/journal.pone.0021550312528821738706Search in Google Scholar

136. Aufauvre J, Biron DG, Vidau C, Fontbonne R, Roudel M, Diogon M, Viguès B, Belzunces LP, Delbac F, Blot N: Parasite-insecticide interactions: a case study of Nosema ceranae and fipronil synergy on honeybee. Sci Rep-UK 2012, 2:326.10.1038/srep00326331022822442753Search in Google Scholar

137. Aufauvre J, Misme-Aucouturier B, Viguès B, Texier C, Delbac F, Blot N: Transcriptome analyses of the honeybee response to Nosema ceranae and insecticides. PLoS ONE 2014, 9:e91686.10.1371/journal.pone.0091686396015724646894Search in Google Scholar

138. Glavinic U, Tesovnik T, Stevanovic J, Zorc M, Cizelj I, Stanimirovic Z, Narat M: Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae. PeerJ 2019, 7:e632510.7717/peerj.6325637191730775168Search in Google Scholar

139. Retschnig G, Neumann P, Williams GR: Thiacloprid–Nosema ceranae interactions in honey bees: Host survivorship but not parasite reproduction is dependent on pesticide dose. J Invertebr Pathol 2014, 118:18-19.10.1016/j.jip.2014.02.00824594300Search in Google Scholar

140. Blacquiere T, Smagghe G, van Gestel CAM, Mommaerts V: Neonicotinoids in bees: A review on concentrations, side-effects and risk assessment. Ecotoxicology 2012, 21:973–992.10.1007/s10646-012-0863-x333832522350105Search in Google Scholar

141. Van der Sluijs JP, Simon-Delso N, Goulson D, Maxim L, Bonmatin JM, Belzunces LP: Neonicotinoids, bee disorders and the sustainability of pollinator services. Curr Opin Env Sust 2013, 5:293-305.10.1016/j.cosust.2013.05.007Search in Google Scholar

142. Abbo PM, Kawasaki JK, Hamilton M, Cook SC, DeGrandi-Hoffman G, Li WF, Liu J, Chen YP: Effects of imidacloprid and Varroa destructor on survival and health of European honey bees, Apis mellifera. Insect Sci 2017, 24:467-477.10.1111/1744-7917.1233526990560Search in Google Scholar

143. Tison L, Hahn ML, Holtz S, Rößner A, Greggers U, Bischoff G, Menzel R: Honey bees’ behavior is impaired by chronic exposure to the neonicotinoid thiacloprid in the field. Environ Sci Technol 2016, 50:7218-7227.10.1021/acs.est.6b0265827268938Search in Google Scholar

144. Straub L, Villamar-Bouza L, Bruckner S, Chantawannakul P, Gauthier L, Khongphinitbunjong K, Retschnig G, Troxler A, Vidondo B, Neumann P, Williams GR: Neonicotinoid insecticides can serve as inadvertent insect contraceptives. Proc R Soc B 2016, 283:20160506.10.1098/rspb.2016.0506497119727466446Search in Google Scholar

145. Di Prisco G, Cavaliere V, Annoscia D, Varricchio P, Caprio E, Nazzi F, Gargiulo G, Pennacchio F: Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees. P Natl Acad Sci USA, 2013, 110:18466-18471.10.1073/pnas.1314923110383198324145453Search in Google Scholar

146. Brandt A, Gorenflo A, Siede R, Meixner M, Büchler R: The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). J Insect Physiol 2016, 86:40-47.10.1016/j.jinsphys.2016.01.00126776096Search in Google Scholar

147. Thompson HM, Fryday SL, Harkin S, Milner S: Potential impacts of synergism in honeybees (Apis mellifera) of exposure to neonicotinoids and sprayed fungicides in crops. Apidologie 2014, 45:545–553.10.1007/s13592-014-0273-6Search in Google Scholar

148. Sgolastra F, Medrzycki P, Bortolotti L, Renzi MT, Tosi S, Bogo G, Teper D, Porrini C, Molowny-Horas R, Bosch J: Synergistic mortality between a neonicotinoid insecticide and an ergosterol-biosynthesis-inhibiting fungicide in three bee species. Pest Manag Sci 2017, 73:1236-1243.10.1002/ps.444927685544Search in Google Scholar

149. Johnson RM, Dahlgren L, Siegfried BD, Ellis MD: Acaricide, fungicide and drug interactions in honey bees (Apis mellifera). PLoS ONE 2013, 8:e54092.10.1371/journal.pone.0054092355850223382869Search in Google Scholar

150. Lambert O, Piroux M, Puyo S, Thorin C, L’Hostis M, Wiest L, Bulete A, Delbac F, Pouliquen H: Widespread occurrence of chemical residues in beehive matrices from apiaries located in different landscapes of Western France. PLoS ONE 2013, 8:e67007.10.1371/journal.pone.0067007368458423799139Search in Google Scholar

151. Cizelj I, Glavan G, Božič J, Oven I, Mrak V, Narat M: Prochloraz and coumaphos induce different gene expression patterns in three developmental stages of the Carniolan honey bee (Apis mellifera carnica Pollmann). Pestic Biochem Phys 2016, 128:68-75.10.1016/j.pestbp.2015.09.01526969442Search in Google Scholar

152. Seeley T, Tarpy D: Queen promiscuity lowers disease within honeybee colonies. Proc R Soc London Sci - Ser B 2007, 274:67–72.10.1098/rspb.2006.3702167987117015336Search in Google Scholar

153. Higes M, Nozal MJ; Alvaro A, Barrios L, Meana A, Martín-Hernández R, Bernal LJ, Bernal J: The stability and effectiveness of fumagillin in controlling Nosema ceranae (Microsporidia) infection in honey bees (Apis mellifera) under laboratory and field conditions. Apidologie 2011, 42:364-377.10.1007/s13592-011-0003-2Search in Google Scholar

154. Van den Heever JP, Thompson TS, Otto SJ, Curtis JM, Ibrahim A, Pernal SF: Evaluation of Fumagilin-B® and other potential alternative chemotherapies against Nosema ceranae-infected honeybees (Apis mellifera) in cage trial assays. Apidologie 2016a, 47:617-630.10.1007/s13592-015-0409-3Search in Google Scholar

155. Van den Heever JP, Thompson TS, Otto SJ, Curtis JM, Ibrahim A, Pernal SF: The effect of dicyclohexylamine and fumagillin on Nosema ceranae-infected honey bee (Apis mellifera) mortality in cage trial assays. Apidologie 2016b, 47:663-670.10.1007/s13592-015-0411-9Search in Google Scholar

156. van den Heever JP, Thompson TS, Curtis JM, Pernal SF: Determination of dicyclohexylamine and fumagillin in honey by LC-MS/MS. Food Anal Method 2015a, 8:767-777.10.1007/s12161-014-9956-xSearch in Google Scholar

157. van den Heever JP, Thompson TS, Curtis JM, Pernal SF: Stability of dicyclohexylamine and fumagillin in honey. Food Chem 2015b, 179:152-158.10.1016/j.foodchem.2015.01.11125722149Search in Google Scholar

158. Runckel C, Flenniken ML, Engel JC, Ruby JG, Ganem D, Andino R, DeRisi JL: Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia. PLoS ONE 2011, 6:e20656.10.1371/journal.pone.0020656311020521687739Search in Google Scholar

159. Cornman RS, Tarpy DR, Chen Y, Jeffreys L, Lopez D, Pettis JS, Evans JD: Pathogen webs in collapsing honey bee colonies. PLoS ONE 2012, 7:e43562.10.1371/journal.pone.0043562342416522927991Search in Google Scholar

160. Ravoet J, Maharramov J, Meeus I, De Smet L, Wenseleers T, Smagghe G, De Graaf DC: Comprehensive bee pathogen screening in Belgium reveals Crithidia mellificae as a new contributory factor to winter mortality. PLoS ONE 2013, 8:e72443.10.1371/journal.pone.0072443375327523991113Search in Google Scholar

161. Ravoet J, Schwarz RS, Descamp T, Yanez O, Tozkar O, Martin-Hernandez R, De Smet L, DeRisi J, Higes M, Wenseleers T, Schmid-Hempel R, Neumann P, Kadowaki T, Evans JD, de Graaf DC: Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim. J Invertebr Pathol 2015, 130:21-27.10.1016/j.jip.2015.06.00726146231Search in Google Scholar

162. Schwarz RS, Evans JD: Single and mixed-species trypanosome and microsporidia infections elicit distinct, ephemeral cellular and humoral immune responses in honeybees. Dev Comp Immunol 2013, 40:300–310.10.1016/j.dci.2013.03.01023529010Search in Google Scholar

163. Stevanovic J, Vejnovic B, Schwarz R, Aleksic N, Jovanovic N, Stanimirovic Z: Identification and quantification of Lotmaria passim (Trypanosomatidae) in investigation of its prevalence, annual dynamics and relationship with Nosema ceranae (Microsporidia). Book of plenary lectures, XXX Congresso SoIPA: Mutamenti Ambientali e Parassiti, June 26-29, 2018a, Milano, Italy pp. 55-57.Search in Google Scholar

164. Radaković M, Vejnović B, Glavinić U, Aleksić N, Mirilović M, Stanimirović Z, Stevanović J: Oxidative stress increases in honey bees infected with Lotmaria passim. Abstract Book, 8th Congress of Apidology (EURBEE 8), Sept 18-20, 2018, pp. 108, Ghent, Belgium.Search in Google Scholar

165. Aleksic N, Stanimirovic Z, Stevanovic J: External contaminants of honey bee products (invited paper). In Zivadinovic, R. (Ed), Proceedings of the Apimondia Symposium “APIECOTECH SERBIA 2012”, Feb 18-19, 2012, Belgrade, Serbia, pp 46-63.Search in Google Scholar

166. Calatayud-Vernich P, Calatayud F, Simó E, Picó Y: Pesticide residues in honey bees, pollen and beeswax: Assessing beehive exposure. Environmental Pollution 2018, 241:106-114.10.1016/j.envpol.2018.05.06229803024Search in Google Scholar

167. Esteves RJP, Villadelrey MC, Rabajante JF: Determining the optimal distribution of bee colony locations to avoid overpopulation using mixed integer programming. Journal of Nature Studies 2010, 9:79-82.Search in Google Scholar

168. Favre D: Mobile phone-induced honeybee worker piping. Apidologie 2011, 42:270-279.10.1007/s13592-011-0016-xSearch in Google Scholar

169. Favre D: Disturbing honeybees’ behavior with electromagnetic waves: a methodology. J Behav 2017, 2:1010.Search in Google Scholar

170. Barron AB: Death of the bee hive: understanding the failure of an insect society. Curr Opin Insect Sci 2015, 10:45-50.10.1016/j.cois.2015.04.00429588013Search in Google Scholar

171. Archer CR, Köhler A, Pirk CW, Oosthuizen V, Apostolides Z, Nicolson SW: Antioxidant supplementation can reduce the survival costs of excess amino acid intake in honeybees. J Insect Physiol 2014, 71:78-86.10.1016/j.jinsphys.2014.10.00625450562Search in Google Scholar

172. Farjan M, Łopieńska-Biernat E, Lipiński Z, Dmitryjuk M, Żółtowska K: Supplementing with vitamin C the diet of honeybees (Apis mellifera carnica) parasitized with Varroa destructor: effects on antioxidative status. Parasitology 2014, 141:770-776.10.1017/S003118201300212624477034Search in Google Scholar

173. Ptaszyńska AA, Borsuk G, Zdybicka-Barabas A, Cytryńska M, Małek W: Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C?. Parasitol Res 2016, 115:397-406.10.1007/s00436-015-4761-z470009326437644Search in Google Scholar

174. Stevanovic J, Stanimirovic Z, Simeunovic P, Lakic N, Radovic I, Sokovic M, Van Griensven JLD: The effect of Agaricus brasiliensis extract supplementation on honey bee colonies. An Acad Bras Cienc 2018b, 90:219-229.10.1590/0001-376520182015018229424386Search in Google Scholar

175. Amiri E, Strand M, Rueppell O, Tarpy D: Queen quality and the impact of honey bee diseases on queen health: potential for interactions between two major threats to colony health. Insects 2017, 8:48.10.3390/insects8020048549206228481294Search in Google Scholar

176. Evans JD, Lopez DL: Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). J Econ Entomol 2004, 97:752-756.10.1093/jee/97.3.752Search in Google Scholar

177. Chan QW, Melathopoulos AP, Pernal SF, Foster LJ: The innate immune and systemic response in honey bees to a bacterial pathogen, Paenibacillus larvae. BMC Genomics 2009, 10:387.10.1186/1471-2164-10-387290769919695106Search in Google Scholar

178. Azzami K, Ritter W, Tautz J, Beier H: Infection of honey bees with acute bee paralysis virus does not trigger humoral or cellular immune responses. Arch Virol 2012, 157:689-702.10.1007/s00705-012-1223-0331481622258854Search in Google Scholar

179. Gätschenberger H, Azzami K, Tautz J, Beier H: Antibacterial immune competence of honey bees (Apis mellifera) is adapted to different life stages and environmental risks. PLoS ONE 2013, 8:e66415.10.1371/journal.pone.0066415368458623799099Search in Google Scholar